Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology
The present study was to determine optimum conditions for gelatin extraction from the skin of buffalo (Bubalus bubalis) using response surface methodology. A central composite design (CCD) was performed to evaluate the effects of NaOH concentration (X1), pre-treatment time (X2), extraction temperatu...
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| Format: | Article |
| Language: | English English |
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National Library of Medicine
2023
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| Online Access: | https://eprints.ums.edu.my/id/eprint/37481/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/37481/2/FULL%20TEXT.pdf |
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| author | Rabiatul Amirah Ramli Umi Hartina Mohamad Razali Nor Qhairul Izzreen Mohd Noor |
| author_facet | Rabiatul Amirah Ramli Umi Hartina Mohamad Razali Nor Qhairul Izzreen Mohd Noor |
| author_sort | Rabiatul Amirah Ramli |
| collection | UMS |
| description | The present study was to determine optimum conditions for gelatin extraction from the skin of buffalo (Bubalus bubalis) using response surface methodology. A central composite design (CCD) was performed to evaluate the effects of NaOH concentration (X1), pre-treatment time (X2), extraction temperature (X3), and extraction time (X4) on the yield (Y1), gel strength (Y2), and hydroxyproline content (Y3) of the extracted gelatin. The optimal combination of the independent variables for a good gelatin yield with high gel strength and hydroxyproline content was found at X1 (0.77 M), X2 (5.08 h), X3 (62.93 ◦C) and X4 (11.62 h). The experimental values for Y1 (16.91%), Y2 (236.5 g), and Y3 (41.4 g/100 g) were in good agreement with the predicted values of 17.87% yield, 237.80 g gel strength and 41.90 g/100 g of hydroxyproline content. Extraction temperature and extraction time were observed to be the most important factors that influenced the yield, gel strength, and hydroxyproline content, meanwhile pre-treatment time showed negative correlations with the yield and hydroxyproline content of the extracted gelatin. This study demonstrated that manipulation of specific parameters could improve extraction efficiency without compromising the quality of buffalo gelatin, thereby promoting it as an alternative source for gelatin production. |
| first_indexed | 2024-03-06T03:25:46Z |
| format | Article |
| id | ums.eprints-37481 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:25:46Z |
| publishDate | 2023 |
| publisher | National Library of Medicine |
| record_format | dspace |
| spelling | ums.eprints-374812023-10-09T07:47:21Z https://eprints.ums.edu.my/id/eprint/37481/ Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology Rabiatul Amirah Ramli Umi Hartina Mohamad Razali Nor Qhairul Izzreen Mohd Noor SF101-103.5 Brands and branding, and other means of identifying SF402-405 Fur-bearing animals The present study was to determine optimum conditions for gelatin extraction from the skin of buffalo (Bubalus bubalis) using response surface methodology. A central composite design (CCD) was performed to evaluate the effects of NaOH concentration (X1), pre-treatment time (X2), extraction temperature (X3), and extraction time (X4) on the yield (Y1), gel strength (Y2), and hydroxyproline content (Y3) of the extracted gelatin. The optimal combination of the independent variables for a good gelatin yield with high gel strength and hydroxyproline content was found at X1 (0.77 M), X2 (5.08 h), X3 (62.93 ◦C) and X4 (11.62 h). The experimental values for Y1 (16.91%), Y2 (236.5 g), and Y3 (41.4 g/100 g) were in good agreement with the predicted values of 17.87% yield, 237.80 g gel strength and 41.90 g/100 g of hydroxyproline content. Extraction temperature and extraction time were observed to be the most important factors that influenced the yield, gel strength, and hydroxyproline content, meanwhile pre-treatment time showed negative correlations with the yield and hydroxyproline content of the extracted gelatin. This study demonstrated that manipulation of specific parameters could improve extraction efficiency without compromising the quality of buffalo gelatin, thereby promoting it as an alternative source for gelatin production. National Library of Medicine 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/37481/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/37481/2/FULL%20TEXT.pdf Rabiatul Amirah Ramli and Umi Hartina Mohamad Razali and Nor Qhairul Izzreen Mohd Noor (2023) Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology. Heliyon. pp. 1-10. https://doi.org/10.1016/j.heliyon.2023.e14367 |
| spellingShingle | SF101-103.5 Brands and branding, and other means of identifying SF402-405 Fur-bearing animals Rabiatul Amirah Ramli Umi Hartina Mohamad Razali Nor Qhairul Izzreen Mohd Noor Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology |
| title | Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology |
| title_full | Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology |
| title_fullStr | Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology |
| title_full_unstemmed | Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology |
| title_short | Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology |
| title_sort | optimization of extraction conditions of gelatin from buffalo bubalus bubalis skins using response surface methodology |
| topic | SF101-103.5 Brands and branding, and other means of identifying SF402-405 Fur-bearing animals |
| url | https://eprints.ums.edu.my/id/eprint/37481/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/37481/2/FULL%20TEXT.pdf |
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