A protocol and training guidelines for mosquito sampling in remote areas with limited power supply
Mosquito-borne diseases pose a significant threat in many Southeast Asian countries, particularly through the sylvatic cycle, which has a wildlife reservoir in forests and rural areas. Studying the composition and diversity of vectors and pathogen transmission is especially challenging in forests an...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier B.V.
2024
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/38910/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/38910/2/FULL%20TEXT.pdf |
| _version_ | 1811136381584408576 |
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| author | Ong, Song-Quan Mahadimenakbar Mohamed Dawood Homathevi Rahman Mohd Farid Alias Mohd Arshil Moideen Lee, Ping Chin Jodi M Fiorenzanoe Nathaniel Christy Thomas McGlynn Noel Cote |
| author_facet | Ong, Song-Quan Mahadimenakbar Mohamed Dawood Homathevi Rahman Mohd Farid Alias Mohd Arshil Moideen Lee, Ping Chin Jodi M Fiorenzanoe Nathaniel Christy Thomas McGlynn Noel Cote |
| author_sort | Ong, Song-Quan |
| collection | UMS |
| description | Mosquito-borne diseases pose a significant threat in many Southeast Asian countries, particularly through the sylvatic cycle, which has a wildlife reservoir in forests and rural areas. Studying the composition and diversity of vectors and pathogen transmission is especially challenging in forests and rural areas due to their remoteness, limited accessibility, lack of power, and underdeveloped infrastructure. This study is based on the WHO mosquito sampling protocol, modifies technical details to support mosquito collection in difficult-to-access and resource-limited areas. Specifically, we describe the procedure for using rechargeable lithium batteries and solar panels to power the mosquito traps, demonstrate a workflow for processing and storing the mosquitoes in a -20 °C freezer, data management tools including microclimate data, and quality assurance processes to ensure the validity and reliability of the results. A pre- and post-test was utilized to measure participant knowledge levels. Additional research is needed to validate this protocol for monitoring vector-borne diseases in hard-to-reach areas within other countries and settings. |
| first_indexed | 2024-09-24T00:49:41Z |
| format | Article |
| id | ums.eprints-38910 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-09-24T00:49:41Z |
| publishDate | 2024 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | ums.eprints-389102024-06-21T07:15:28Z https://eprints.ums.edu.my/id/eprint/38910/ A protocol and training guidelines for mosquito sampling in remote areas with limited power supply Ong, Song-Quan Mahadimenakbar Mohamed Dawood Homathevi Rahman Mohd Farid Alias Mohd Arshil Moideen Lee, Ping Chin Jodi M Fiorenzanoe Nathaniel Christy Thomas McGlynn Noel Cote QL461-599.82 Insects RC109-216 Infectious and parasitic diseases Mosquito-borne diseases pose a significant threat in many Southeast Asian countries, particularly through the sylvatic cycle, which has a wildlife reservoir in forests and rural areas. Studying the composition and diversity of vectors and pathogen transmission is especially challenging in forests and rural areas due to their remoteness, limited accessibility, lack of power, and underdeveloped infrastructure. This study is based on the WHO mosquito sampling protocol, modifies technical details to support mosquito collection in difficult-to-access and resource-limited areas. Specifically, we describe the procedure for using rechargeable lithium batteries and solar panels to power the mosquito traps, demonstrate a workflow for processing and storing the mosquitoes in a -20 °C freezer, data management tools including microclimate data, and quality assurance processes to ensure the validity and reliability of the results. A pre- and post-test was utilized to measure participant knowledge levels. Additional research is needed to validate this protocol for monitoring vector-borne diseases in hard-to-reach areas within other countries and settings. Elsevier B.V. 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38910/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/38910/2/FULL%20TEXT.pdf Ong, Song-Quan and Mahadimenakbar Mohamed Dawood and Homathevi Rahman and Mohd Farid Alias and Mohd Arshil Moideen and Lee, Ping Chin and Jodi M Fiorenzanoe and Nathaniel Christy and Thomas McGlynn and Noel Cote (2024) A protocol and training guidelines for mosquito sampling in remote areas with limited power supply. MethodsX, 12. pp. 1-7. ISSN 2215-0161 https://doi.org/10.1016/j.mex.2024.102563 |
| spellingShingle | QL461-599.82 Insects RC109-216 Infectious and parasitic diseases Ong, Song-Quan Mahadimenakbar Mohamed Dawood Homathevi Rahman Mohd Farid Alias Mohd Arshil Moideen Lee, Ping Chin Jodi M Fiorenzanoe Nathaniel Christy Thomas McGlynn Noel Cote A protocol and training guidelines for mosquito sampling in remote areas with limited power supply |
| title | A protocol and training guidelines for mosquito sampling in remote areas with limited power supply |
| title_full | A protocol and training guidelines for mosquito sampling in remote areas with limited power supply |
| title_fullStr | A protocol and training guidelines for mosquito sampling in remote areas with limited power supply |
| title_full_unstemmed | A protocol and training guidelines for mosquito sampling in remote areas with limited power supply |
| title_short | A protocol and training guidelines for mosquito sampling in remote areas with limited power supply |
| title_sort | protocol and training guidelines for mosquito sampling in remote areas with limited power supply |
| topic | QL461-599.82 Insects RC109-216 Infectious and parasitic diseases |
| url | https://eprints.ums.edu.my/id/eprint/38910/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/38910/2/FULL%20TEXT.pdf |
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