Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions
Cassia fistula Linn. (Golden Shower Tree; Local name: Rajah Kayu) is a mediumsized tree belongs to the family Leguminosae. Its different plant portions are used in traditional treatment of various diseases and common ailments in different parts of the world since time immemorial, however still lesse...
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| Format: | Thesis |
| Language: | English English |
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2014
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| Online Access: | https://eprints.ums.edu.my/id/eprint/38950/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38950/2/FULLTEXT.pdf |
| _version_ | 1825715624185167872 |
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| author | Lai, Tze Kiat |
| author_facet | Lai, Tze Kiat |
| author_sort | Lai, Tze Kiat |
| collection | UMS |
| description | Cassia fistula Linn. (Golden Shower Tree; Local name: Rajah Kayu) is a mediumsized tree belongs to the family Leguminosae. Its different plant portions are used in traditional treatment of various diseases and common ailments in different parts of the world since time immemorial, however still lesser known in Malaysia. In this project, methanolic extracts from different portions (bark, stem, leaf and root) of different age classes, namely class 1 (2-3 years), class 2 (5-10 years) and class 3 (10-15 years) of C. fistula were studied for their total extractives yield (TE) and Stiasny Number (SN) were analyzed. The antioxidant activity (AA), total phenolic content (TPC), and total tannin content (TTC) of plant extracts were analysed using 1,1-Diphenyl-2-Picryl-Hydrazyl (DPPH) Free Radical Scavenging Method and Folin- Ciocalteau Method respectively. Meanwhile, the antifungal activity (AF) of C. fistula extracts against 5 types of wood-rotting fungi, namely Pycnoporus sanguineus (PS), Microporus xanthopus (MX), Coniophora puteana (CP), Trametes spp. (T) and Stereum ostrea (SO) was determined using Poisoned Culture Medium technique. The bark portion of C. fistula produced the highest TE (3.24± 0.56%) and SN (77.77± 0.95%) compared to other portions. Meanwhile, TPC and TTC for C. fistula extract ranged from 4.58± 0.00% to 21.21± 0.05% and 1.18± 0.00% to 3.81± 0.00% respectively. The bark portion of C. fistula showed the highest AA with lowest IC50 (4.04± 0.05 mg/ml – 4.16± 0.03 mg/ml) compared to other portions. The AF showed by C. fistula extracts against wood rotting fungi was considered low and less effective. The highest inhibition activity obtained in this study was 37.75% (<50% inhibition). C. fistula extract was inactive against fungi MX. The overall study showed that, different tree portions displayed greater influences on the chemical characteristic, antioxidant and antifungal activities of C. fistula compared with different tree age classes. Bark extracts of C. fistula demonstrated a potential as natural tannin-adhesives application. The study reported that, C. fistula extracts possessed potential pharmacological properties such as AA and AF against wood rotting fungi. However, further investigation and research on AF of C. fistula against wood rotting fungi is recommended in order to identify potential specific compounds and their exact mechanism. |
| first_indexed | 2024-09-24T00:49:47Z |
| format | Thesis |
| id | ums.eprints-38950 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-09-24T00:49:47Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ums.eprints-389502024-06-27T07:34:00Z https://eprints.ums.edu.my/id/eprint/38950/ Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions Lai, Tze Kiat QK710-899 Plant physiology Cassia fistula Linn. (Golden Shower Tree; Local name: Rajah Kayu) is a mediumsized tree belongs to the family Leguminosae. Its different plant portions are used in traditional treatment of various diseases and common ailments in different parts of the world since time immemorial, however still lesser known in Malaysia. In this project, methanolic extracts from different portions (bark, stem, leaf and root) of different age classes, namely class 1 (2-3 years), class 2 (5-10 years) and class 3 (10-15 years) of C. fistula were studied for their total extractives yield (TE) and Stiasny Number (SN) were analyzed. The antioxidant activity (AA), total phenolic content (TPC), and total tannin content (TTC) of plant extracts were analysed using 1,1-Diphenyl-2-Picryl-Hydrazyl (DPPH) Free Radical Scavenging Method and Folin- Ciocalteau Method respectively. Meanwhile, the antifungal activity (AF) of C. fistula extracts against 5 types of wood-rotting fungi, namely Pycnoporus sanguineus (PS), Microporus xanthopus (MX), Coniophora puteana (CP), Trametes spp. (T) and Stereum ostrea (SO) was determined using Poisoned Culture Medium technique. The bark portion of C. fistula produced the highest TE (3.24± 0.56%) and SN (77.77± 0.95%) compared to other portions. Meanwhile, TPC and TTC for C. fistula extract ranged from 4.58± 0.00% to 21.21± 0.05% and 1.18± 0.00% to 3.81± 0.00% respectively. The bark portion of C. fistula showed the highest AA with lowest IC50 (4.04± 0.05 mg/ml – 4.16± 0.03 mg/ml) compared to other portions. The AF showed by C. fistula extracts against wood rotting fungi was considered low and less effective. The highest inhibition activity obtained in this study was 37.75% (<50% inhibition). C. fistula extract was inactive against fungi MX. The overall study showed that, different tree portions displayed greater influences on the chemical characteristic, antioxidant and antifungal activities of C. fistula compared with different tree age classes. Bark extracts of C. fistula demonstrated a potential as natural tannin-adhesives application. The study reported that, C. fistula extracts possessed potential pharmacological properties such as AA and AF against wood rotting fungi. However, further investigation and research on AF of C. fistula against wood rotting fungi is recommended in order to identify potential specific compounds and their exact mechanism. 2014 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38950/1/24%20PAGES.pdf text en https://eprints.ums.edu.my/id/eprint/38950/2/FULLTEXT.pdf Lai, Tze Kiat (2014) Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions. Masters thesis, Universiti Malaysia Sabah. |
| spellingShingle | QK710-899 Plant physiology Lai, Tze Kiat Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions |
| title | Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions |
| title_full | Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions |
| title_fullStr | Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions |
| title_full_unstemmed | Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions |
| title_short | Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions |
| title_sort | chemical characteristics antioxidant and antifungal activities of cassia fistula linn at different tree ages and portions |
| topic | QK710-899 Plant physiology |
| url | https://eprints.ums.edu.my/id/eprint/38950/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38950/2/FULLTEXT.pdf |
| work_keys_str_mv | AT laitzekiat chemicalcharacteristicsantioxidantandantifungalactivitiesofcassiafistulalinnatdifferenttreeagesandportions |