Unicorn fish (Naso reticulatus Randall, 2001) skin collagens prepared using two pepsin sources: An assessment on physicochemical characteristics
Fish collagens have gained considerable attention from numerous researchers due to their attractive traits and are more acceptable from most religious beliefs. This paper aimed to extract and characterize collagens from the skins of unicorn fish (N. reticulatus) influenced by pepsin from porcine (UC...
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| Format: | Article |
| Language: | English English |
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Indonesian Society for Knowledge and Human Development
2024
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| Online Access: | https://eprints.ums.edu.my/id/eprint/39187/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/39187/2/FULL%20TEXT.pdf |
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| author | Claire Cynthia Anak Patrick Abdul Aziz Jaziri Ahmad Hazim Abdul Aziz Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Rahmi Nurdiani Nurul Huda |
| author_facet | Claire Cynthia Anak Patrick Abdul Aziz Jaziri Ahmad Hazim Abdul Aziz Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Rahmi Nurdiani Nurul Huda |
| author_sort | Claire Cynthia Anak Patrick |
| collection | UMS |
| description | Fish collagens have gained considerable attention from numerous researchers due to their attractive traits and are more acceptable from most religious beliefs. This paper aimed to extract and characterize collagens from the skins of unicorn fish (N. reticulatus) influenced by pepsin from porcine (UCP) and pepsin from bovine (UCB). The yield of the UCP sample (15.60%) was significantly higher (P<0.05) compared to the UCB (10.40%). In addition to this, the swelling value of two collagens showed significant differences (P<0.05), with a greater percentage obtained in UCP 9261.23%) rather than UCB (196.75%). Both UCP and UCB were classified as type I collagen owing to the existence of two alpha chains under SDS-polyacrylamide gel electrophoresis. Under infrared and ultraviolet-visible parameters, the triple helical structure of collagens prepared using pepsin from porcine and bovine was preserved, and it was comparable to previous findings in fish collagen literature. All samples showed a different thermostability value, the higher one observed in the UCP (43.63°C) compared to the UCB sample (35.25°C), and their variations of thermostability were in agreement with the hydroxyproline content of UCP (83.57 mg/g) and UCB (81.47 mg/g). The unicorn fish (N. reticulatus) skin may be used as a good source of collagen, mainly utilized for industrial perspective. |
| first_indexed | 2024-09-24T00:50:24Z |
| format | Article |
| id | ums.eprints-39187 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-09-24T00:50:24Z |
| publishDate | 2024 |
| publisher | Indonesian Society for Knowledge and Human Development |
| record_format | dspace |
| spelling | ums.eprints-391872024-07-16T07:47:24Z https://eprints.ums.edu.my/id/eprint/39187/ Unicorn fish (Naso reticulatus Randall, 2001) skin collagens prepared using two pepsin sources: An assessment on physicochemical characteristics Claire Cynthia Anak Patrick Abdul Aziz Jaziri Ahmad Hazim Abdul Aziz Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Rahmi Nurdiani Nurul Huda QL1-355 General Including geographical distribution QL360-599.82 Invertebrates Fish collagens have gained considerable attention from numerous researchers due to their attractive traits and are more acceptable from most religious beliefs. This paper aimed to extract and characterize collagens from the skins of unicorn fish (N. reticulatus) influenced by pepsin from porcine (UCP) and pepsin from bovine (UCB). The yield of the UCP sample (15.60%) was significantly higher (P<0.05) compared to the UCB (10.40%). In addition to this, the swelling value of two collagens showed significant differences (P<0.05), with a greater percentage obtained in UCP 9261.23%) rather than UCB (196.75%). Both UCP and UCB were classified as type I collagen owing to the existence of two alpha chains under SDS-polyacrylamide gel electrophoresis. Under infrared and ultraviolet-visible parameters, the triple helical structure of collagens prepared using pepsin from porcine and bovine was preserved, and it was comparable to previous findings in fish collagen literature. All samples showed a different thermostability value, the higher one observed in the UCP (43.63°C) compared to the UCB sample (35.25°C), and their variations of thermostability were in agreement with the hydroxyproline content of UCP (83.57 mg/g) and UCB (81.47 mg/g). The unicorn fish (N. reticulatus) skin may be used as a good source of collagen, mainly utilized for industrial perspective. Indonesian Society for Knowledge and Human Development 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/39187/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/39187/2/FULL%20TEXT.pdf Claire Cynthia Anak Patrick and Abdul Aziz Jaziri and Ahmad Hazim Abdul Aziz and Rossita Shapawi and Ruzaidi Azli Mohd Mokhtar and Wan Norhana Md. Noordin and Rahmi Nurdiani and Nurul Huda (2024) Unicorn fish (Naso reticulatus Randall, 2001) skin collagens prepared using two pepsin sources: An assessment on physicochemical characteristics. International Journal on Advanced Science, Engineering and Information Technology, 14 (1). pp. 1-7. ISSN 2088-5334 https://doi.org/10.18517/ijaseit.14.1.19656 |
| spellingShingle | QL1-355 General Including geographical distribution QL360-599.82 Invertebrates Claire Cynthia Anak Patrick Abdul Aziz Jaziri Ahmad Hazim Abdul Aziz Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Rahmi Nurdiani Nurul Huda Unicorn fish (Naso reticulatus Randall, 2001) skin collagens prepared using two pepsin sources: An assessment on physicochemical characteristics |
| title | Unicorn fish (Naso reticulatus Randall, 2001) skin collagens prepared using two pepsin sources: An assessment on physicochemical characteristics |
| title_full | Unicorn fish (Naso reticulatus Randall, 2001) skin collagens prepared using two pepsin sources: An assessment on physicochemical characteristics |
| title_fullStr | Unicorn fish (Naso reticulatus Randall, 2001) skin collagens prepared using two pepsin sources: An assessment on physicochemical characteristics |
| title_full_unstemmed | Unicorn fish (Naso reticulatus Randall, 2001) skin collagens prepared using two pepsin sources: An assessment on physicochemical characteristics |
| title_short | Unicorn fish (Naso reticulatus Randall, 2001) skin collagens prepared using two pepsin sources: An assessment on physicochemical characteristics |
| title_sort | unicorn fish naso reticulatus randall 2001 skin collagens prepared using two pepsin sources an assessment on physicochemical characteristics |
| topic | QL1-355 General Including geographical distribution QL360-599.82 Invertebrates |
| url | https://eprints.ums.edu.my/id/eprint/39187/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/39187/2/FULL%20TEXT.pdf |
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