Characterization and bioaugmentation of stranded tar balls in subtidal sediments of marintaman beach Sipitang Sabah by using consortia of locally isolated beneficial microorganisms (LIBeM)
Oil spill is one of the environmental pollutions that commonly occurs along the coastal area. Tar-balls are one of the products come from the oil spill pollution. Tar-balls pollution was monitored at 10 points along the coastline of Marintaman Beach at Sipitang, Sabah were observed weekly in Februar...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2020
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| Online Access: | https://eprints.ums.edu.my/id/eprint/40716/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/40716/2/FULLTEXT.pdf |
| Summary: | Oil spill is one of the environmental pollutions that commonly occurs along the coastal area. Tar-balls are one of the products come from the oil spill pollution. Tar-balls pollution was monitored at 10 points along the coastline of Marintaman Beach at Sipitang, Sabah were observed weekly in February 2016. This study is aimed to determine the physical characteristics, composition and concentration of Polycyclic Aromatic Hydrocarbons (PAHs} of the existence of tar balls in that monitored week. The total of tar-balls collected were 227 pieces (n=227). The shape of collected tar-balls was not irregular shape arid the sizes of the tar-balls in range of 0.1 cm to 6.9 cm. The composition and concentration of PAHs in outer and inner layer of tar-ball was determined, top three compounds are benzo [g,h,i] perylene (196.58 ppm), flourene (141.8 ppm}, dibenzo [a,h] anthracene (101.95 ppm}, and indeno [1,2,3- c,d] pyrene (85.49 ppm) and they are high ly found in the inner layers of the sample. The biodegradation efficiency, rate and environmental parameters were determined during the biodegradation of tar-balls by LIBeM in liquid fom1ulation. The biodegradation Consortia UBeM ( C tropica/is-RETL-Crl + C vio/aceunrMAB- Crl + P. aemginosa-BAS-Cil) showed the degradation of TPH of natural attenuation were 81%-fold with 83.36% and 19.11 % respectively after 84-day periods. For CFU, the range of cell counts that suit the treatment was exceeding to 104 with maximum value recorded 5.0 x 107 CFU/ml . For pH, it was observed that the tarballs treated well with consortia LIBeM and recorded an average pH was 6.00 to 6.86 respectively during the treatment. The treatment of tar-balls that treated with LIBeM consortia were recorded the soil moisture was below the range with 8% to 9%. For temperature Oil spill is one of the environmental pollutions that commonly occurs along the coastal area. Tar-balls are one of the products come from the oil spill pollution. Tar- balls pollution was monitored at 10 points along the coastline of Marintaman Beach at Sipitang, Sabah were observed weekly in February 2016. This study is aimed to determine the physical characteristics, composition and concentration of Polycyclic Aromatic Hydrocarbons (PAHs) of the existence of tar balls in that monitored week. The total of tar-balls collected were 227 pieces (n=227). The shape of collected tar- balls was not irregular shape and the sizes of the tar-balls in range of 0.1 cm to 6.9 cm. The composition and concentration of PAHs in outer and inner layer of tar-ball was determined, top three compounds are benzo [g,h,i] perylene (196.58 ppm), flourene (141.8 ppm), dibenzo [a,h] anthracene (101.95 ppm), and indeno [1,2,3- c,d] pyrene (85.49 ppm) and they are highly found in the inner layers of the sample. The biodegradation efficiency, rate and environmental parameters were determined during the biodegradation of tar-balls by LIBeM in liquid formulation. The Cbiodegradation Consortia LIBeM ( .o ls-RETL-Cr1 + laceum-MAB- Cr1 + P.aeruginosa-BAS-Cr1) showed the degradation of TPH of natural attenuation were 81%-fold with 83.36% and 19.11% respectively after 84-day periods. For CFU, the range of cell counts that suit the treatment was exceeding to 104 with maximum value recorded 5.0 x 107 CFU/mL . For pH, it was observed that the tar- balls treated well with consortia LIBeM and recorded an average pH was 6.00 to 6.86 respectively during the treatment. The treatment of tar-balls that treated with LIBeM consortia were recorded the soil moisture was below the range with 8% to 9%. For temperature the trends of temperature in tar-balls treatment with consortia LIBeM not showed a large deviation in the range 28-34°C. The biodegradation profile and ratio were monitored using the Gas Chromatography- Mass Spectrometry (GC-MS). The ratio shows that the biodegradation of treatment tar-balls had taken place during the specified biodegradation study. ASP- Biodegradation by LIBeM-LIQ formulation of C. o lsRETL-Cr1, inosa BAS-Cr1 and C.valaceumMAB-Cr1 has a great potential as an integrated approach for treatment of tar-balls after oil-spill in marine ecosystem.the trernds of temperature in tar-balls treatment with consortia LIBeM not showed a large deviation in the range 28-34°C. The biodegradation profile and ratio were monitored using the Gas ChromatographyMass Spectrometry (GC-MS}. The ratio shows that the biodegradation of treatment tar-balls had taken place during the specified biodegradation study. ASPBiodegradation by LIBeM-LIQ formulation ,of C tropica/is RETL-Crl, P. aeruginosa BAS-crl and C va!aceum MAB-crl has a great potential as an integrated approach for treatment of tar-balls after oil-spill in marine ecosystem. |
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