Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores
Dietary assessment plays a crucial role in comprehending the ecological dynamics and nutritional needs of herbivores and omnivores. The metabarcoding technique has emerged as a potent tool for exploring the dietary composition of these animals. However, various metabarcoding techniques have been dev...
| Main Authors: | , , , , , , , , , |
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| Format: | Proceedings |
| Language: | English English |
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EDP Sciences
2024
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/40946/5/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/40946/4/FULL%20TEXT.pdf |
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| author | Nur Hartini Sariyati Hidayah Haris Farah Farhana Ramli Suliadi Firdaus Sufahani Badrul Munir Md-Zain Salmah Yaakop Abd Rahman Mohd-Ridwan Nor Rahman Aifat Mohd Sanusi Mohamed Ibnu Maryanto |
| author_facet | Nur Hartini Sariyati Hidayah Haris Farah Farhana Ramli Suliadi Firdaus Sufahani Badrul Munir Md-Zain Salmah Yaakop Abd Rahman Mohd-Ridwan Nor Rahman Aifat Mohd Sanusi Mohamed Ibnu Maryanto |
| author_sort | Nur Hartini Sariyati |
| collection | UMS |
| description | Dietary assessment plays a crucial role in comprehending the ecological dynamics and nutritional needs of herbivores and omnivores. The metabarcoding technique has emerged as a potent tool for exploring the dietary composition of these animals. However, various metabarcoding techniques have been developed, each with its own advantages and limitations. This study aims to compare the performance of different metabarcoding techniques in herbivores and omnivores diet. We systematically reviewed 159 published manuscripts in Scopus and Google Scholar, and thematic analysis was conducted across several categories, including the marker, platform, and database utilized. Preliminary findings reveal significant variations among metabarcoding techniques across these two animal groups. The trnL gene exhibited higher taxonomic resolution for herbivorous species, whereas the combination of the 'trnL + 16s rRNA' gene exhibited superior performance for omnivorous species. The Illumina platform emerged as the most commonly used method for analyzing the diets of both herbivores and omnivores, with the primary reference database being the National Centre for Biotechnology Information (NCBI). This study offers valuable insights into the strengths and limitations of different metabarcoding techniques for dietary assessment in herbivores and omnivores and optimizing metabarcoding protocols, facilitating more precise and reliable diet analyses within these ecological groups. |
| first_indexed | 2024-09-24T00:52:38Z |
| format | Proceedings |
| id | ums.eprints-40946 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-09-24T00:52:38Z |
| publishDate | 2024 |
| publisher | EDP Sciences |
| record_format | dspace |
| spelling | ums.eprints-409462024-09-09T04:27:52Z https://eprints.ums.edu.my/id/eprint/40946/ Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores Nur Hartini Sariyati Hidayah Haris Farah Farhana Ramli Suliadi Firdaus Sufahani Badrul Munir Md-Zain Salmah Yaakop Abd Rahman Mohd-Ridwan Nor Rahman Aifat Mohd Sanusi Mohamed Ibnu Maryanto QH540-549.5 Ecology QP501-801 Animal biochemistry Dietary assessment plays a crucial role in comprehending the ecological dynamics and nutritional needs of herbivores and omnivores. The metabarcoding technique has emerged as a potent tool for exploring the dietary composition of these animals. However, various metabarcoding techniques have been developed, each with its own advantages and limitations. This study aims to compare the performance of different metabarcoding techniques in herbivores and omnivores diet. We systematically reviewed 159 published manuscripts in Scopus and Google Scholar, and thematic analysis was conducted across several categories, including the marker, platform, and database utilized. Preliminary findings reveal significant variations among metabarcoding techniques across these two animal groups. The trnL gene exhibited higher taxonomic resolution for herbivorous species, whereas the combination of the 'trnL + 16s rRNA' gene exhibited superior performance for omnivorous species. The Illumina platform emerged as the most commonly used method for analyzing the diets of both herbivores and omnivores, with the primary reference database being the National Centre for Biotechnology Information (NCBI). This study offers valuable insights into the strengths and limitations of different metabarcoding techniques for dietary assessment in herbivores and omnivores and optimizing metabarcoding protocols, facilitating more precise and reliable diet analyses within these ecological groups. EDP Sciences 2024-03-25 Proceedings PeerReviewed text en https://eprints.ums.edu.my/id/eprint/40946/5/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/40946/4/FULL%20TEXT.pdf Nur Hartini Sariyati and Hidayah Haris and Farah Farhana Ramli and Suliadi Firdaus Sufahani and Badrul Munir Md-Zain and Salmah Yaakop and Abd Rahman Mohd-Ridwan and Nor Rahman Aifat and Mohd Sanusi Mohamed and Ibnu Maryanto (2024) Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores. https://www.bio-conferences.org/articles/bioconf/abs/2024/13/bioconf_icbs2024_01002/bioconf_icbs2024_01002.html |
| spellingShingle | QH540-549.5 Ecology QP501-801 Animal biochemistry Nur Hartini Sariyati Hidayah Haris Farah Farhana Ramli Suliadi Firdaus Sufahani Badrul Munir Md-Zain Salmah Yaakop Abd Rahman Mohd-Ridwan Nor Rahman Aifat Mohd Sanusi Mohamed Ibnu Maryanto Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_full | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_fullStr | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_full_unstemmed | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_short | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_sort | comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| topic | QH540-549.5 Ecology QP501-801 Animal biochemistry |
| url | https://eprints.ums.edu.my/id/eprint/40946/5/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/40946/4/FULL%20TEXT.pdf |
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