In vitro antifungal activity of thiram against Ganoderma boninense
Basal stem rot (BSR) which is caused by Ganoderma boninense (GB) is the most serious disease faced by oil palm industry, especially in Malaysia. To date, there is no satisfactory control measure for this disease, and researchers are investigating different approaches in managing the disease. The cur...
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| Format: | Article |
| Language: | English English |
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e-VIBS, Faculty of Science and Natural Resources
2020
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| Online Access: | https://eprints.ums.edu.my/id/eprint/40968/5/ABSTRACT1.pdf https://eprints.ums.edu.my/id/eprint/40968/2/FULL%20TEXT.pdf |
| _version_ | 1811136543369199616 |
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| author | Elleiancly Yuli Jedol Dayou Chong, Khim Phin |
| author_facet | Elleiancly Yuli Jedol Dayou Chong, Khim Phin |
| author_sort | Elleiancly Yuli |
| collection | UMS |
| description | Basal stem rot (BSR) which is caused by Ganoderma boninense (GB) is the most serious disease faced by oil palm industry, especially in Malaysia. To date, there is no satisfactory control measure for this disease, and researchers are investigating different approaches in managing the disease. The current study investigates the antifungal properties of Tetramethyl thiuram disulfide or commonly known as thiram, against GB. The in vitro antifungal activity of thiram were expressed in inhibition of GB mycelia growth on Potato Dextrose Agar (PDA) incorporated with different concentrations of thiram (0.010, 0.012, 0.014, 0.016, 0.018, 0.020, and 0.030 mg/ml). The lower concentrations of thiram, such as 0.010 mg/ml, failed to inhibit the growth of GB completely. However, higher concentrations of thiram (0.012 to 0.020 mg/ml) significantly slow the growth rate of GB in comparison to control (without thiram). The concentration of thiram at 0.030 mg/ml completely inhibits the growth of GB. To further evaluate the effectiveness of the treatment, the GB treated mycelia were examined for their ergosterol content using HPLC. The result shows that a higher amount of ergosterol content was found in less effective treatments, and no ergosterol was found in sample when GB is completely inhibited. |
| first_indexed | 2024-09-24T00:52:41Z |
| format | Article |
| id | ums.eprints-40968 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-09-24T00:52:41Z |
| publishDate | 2020 |
| publisher | e-VIBS, Faculty of Science and Natural Resources |
| record_format | dspace |
| spelling | ums.eprints-409682024-09-05T02:22:51Z https://eprints.ums.edu.my/id/eprint/40968/ In vitro antifungal activity of thiram against Ganoderma boninense Elleiancly Yuli Jedol Dayou Chong, Khim Phin QK474.8-495 Spermatophyta. Phanerogams SB1-1110 Plant culture Basal stem rot (BSR) which is caused by Ganoderma boninense (GB) is the most serious disease faced by oil palm industry, especially in Malaysia. To date, there is no satisfactory control measure for this disease, and researchers are investigating different approaches in managing the disease. The current study investigates the antifungal properties of Tetramethyl thiuram disulfide or commonly known as thiram, against GB. The in vitro antifungal activity of thiram were expressed in inhibition of GB mycelia growth on Potato Dextrose Agar (PDA) incorporated with different concentrations of thiram (0.010, 0.012, 0.014, 0.016, 0.018, 0.020, and 0.030 mg/ml). The lower concentrations of thiram, such as 0.010 mg/ml, failed to inhibit the growth of GB completely. However, higher concentrations of thiram (0.012 to 0.020 mg/ml) significantly slow the growth rate of GB in comparison to control (without thiram). The concentration of thiram at 0.030 mg/ml completely inhibits the growth of GB. To further evaluate the effectiveness of the treatment, the GB treated mycelia were examined for their ergosterol content using HPLC. The result shows that a higher amount of ergosterol content was found in less effective treatments, and no ergosterol was found in sample when GB is completely inhibited. e-VIBS, Faculty of Science and Natural Resources 2020 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/40968/5/ABSTRACT1.pdf text en https://eprints.ums.edu.my/id/eprint/40968/2/FULL%20TEXT.pdf Elleiancly Yuli and Jedol Dayou and Chong, Khim Phin (2020) In vitro antifungal activity of thiram against Ganoderma boninense. Transactions on Science and Technology, 7 (3-2). pp. 159-164. |
| spellingShingle | QK474.8-495 Spermatophyta. Phanerogams SB1-1110 Plant culture Elleiancly Yuli Jedol Dayou Chong, Khim Phin In vitro antifungal activity of thiram against Ganoderma boninense |
| title | In vitro antifungal activity of thiram against Ganoderma boninense |
| title_full | In vitro antifungal activity of thiram against Ganoderma boninense |
| title_fullStr | In vitro antifungal activity of thiram against Ganoderma boninense |
| title_full_unstemmed | In vitro antifungal activity of thiram against Ganoderma boninense |
| title_short | In vitro antifungal activity of thiram against Ganoderma boninense |
| title_sort | in vitro antifungal activity of thiram against ganoderma boninense |
| topic | QK474.8-495 Spermatophyta. Phanerogams SB1-1110 Plant culture |
| url | https://eprints.ums.edu.my/id/eprint/40968/5/ABSTRACT1.pdf https://eprints.ums.edu.my/id/eprint/40968/2/FULL%20TEXT.pdf |
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