Advancing COVID-19 detection: high-performance RNA biosensing via electrical interactions
This research paper investigated the detection of COVID-19 using an Aluminum Interdigitated Electrode (Al-IDE) sensor based on electrical conductivity. The silanization process involved the functionalization step, employing (3-Aminopropyl) triethoxysilane (APTES), while the immobilization process wa...
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| Format: | Article |
| Language: | English English |
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Penerbit UniMAP
2024
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| Online Access: | https://eprints.ums.edu.my/id/eprint/41424/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/41424/2/FULL%20TEXT.pdf |
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| author | Uda Hashim M. N. A. Uda Muhammad Nur Afnan Uda Tijjani Adam Nur Hulwani Ibrahim Chai Chang Yii Lorita Angeline |
| author_facet | Uda Hashim M. N. A. Uda Muhammad Nur Afnan Uda Tijjani Adam Nur Hulwani Ibrahim Chai Chang Yii Lorita Angeline |
| author_sort | Uda Hashim |
| collection | UMS |
| description | This research paper investigated the detection of COVID-19 using an Aluminum Interdigitated Electrode (Al-IDE) sensor based on electrical conductivity. The silanization process involved the functionalization step, employing (3-Aminopropyl) triethoxysilane (APTES), while the immobilization process was facilitated by the RNA Probe specific to COVID-19. To verify its specificity in detection, the functionalized biosensor was tested against single-base mismatches, non-complementary sequences, and complementary sequences. The physical characteristics of the Al-IDE biosensor were examined using both low-power microscopy (LPM) and high-power microscopy (HPM). Additionally, the morphological properties of the biosensor were assessed using atomic force microscopy (AFM). To assess its diagnostic potential, the biosensor's sensitivity was evaluated by exposing it to a range of complementary targets, spanning from 1 femtomolar (fM) to 1 micromolar (µM). The current-voltage (I-V) characteristics of the biosensor were meticulously analyzed at each stage of functionalization bare Al-IDE, silanization, immobilization, and hybridization. This I-V characterization was carried out using a picoammeter voltage source (Keithley 2450), Kickstart software, and a probe station. The results confirmed the biosensor's capability to effectively detect COVID-19 targets within the nanoampere concentration range, demonstrating its success in detecting specific COVID-19 targets at the nanoampere level. |
| first_indexed | 2024-12-09T00:52:28Z |
| format | Article |
| id | ums.eprints-41424 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-12-09T00:52:28Z |
| publishDate | 2024 |
| publisher | Penerbit UniMAP |
| record_format | dspace |
| spelling | ums.eprints-414242024-10-16T06:08:16Z https://eprints.ums.edu.my/id/eprint/41424/ Advancing COVID-19 detection: high-performance RNA biosensing via electrical interactions Uda Hashim M. N. A. Uda Muhammad Nur Afnan Uda Tijjani Adam Nur Hulwani Ibrahim Chai Chang Yii Lorita Angeline RC705-779 Diseases of the respiratory system TA1-2040 Engineering (General). Civil engineering (General) This research paper investigated the detection of COVID-19 using an Aluminum Interdigitated Electrode (Al-IDE) sensor based on electrical conductivity. The silanization process involved the functionalization step, employing (3-Aminopropyl) triethoxysilane (APTES), while the immobilization process was facilitated by the RNA Probe specific to COVID-19. To verify its specificity in detection, the functionalized biosensor was tested against single-base mismatches, non-complementary sequences, and complementary sequences. The physical characteristics of the Al-IDE biosensor were examined using both low-power microscopy (LPM) and high-power microscopy (HPM). Additionally, the morphological properties of the biosensor were assessed using atomic force microscopy (AFM). To assess its diagnostic potential, the biosensor's sensitivity was evaluated by exposing it to a range of complementary targets, spanning from 1 femtomolar (fM) to 1 micromolar (µM). The current-voltage (I-V) characteristics of the biosensor were meticulously analyzed at each stage of functionalization bare Al-IDE, silanization, immobilization, and hybridization. This I-V characterization was carried out using a picoammeter voltage source (Keithley 2450), Kickstart software, and a probe station. The results confirmed the biosensor's capability to effectively detect COVID-19 targets within the nanoampere concentration range, demonstrating its success in detecting specific COVID-19 targets at the nanoampere level. Penerbit UniMAP 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/41424/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/41424/2/FULL%20TEXT.pdf Uda Hashim and M. N. A. Uda and Muhammad Nur Afnan Uda and Tijjani Adam and Nur Hulwani Ibrahim and Chai Chang Yii and Lorita Angeline (2024) Advancing COVID-19 detection: high-performance RNA biosensing via electrical interactions. International Journal of Nanoelectronics and Materials (IJNeaM), 17. pp. 229-235. ISSN 0198-5761 https://doi.org/10.58915/ijneam.v17iJune.862 |
| spellingShingle | RC705-779 Diseases of the respiratory system TA1-2040 Engineering (General). Civil engineering (General) Uda Hashim M. N. A. Uda Muhammad Nur Afnan Uda Tijjani Adam Nur Hulwani Ibrahim Chai Chang Yii Lorita Angeline Advancing COVID-19 detection: high-performance RNA biosensing via electrical interactions |
| title | Advancing COVID-19 detection: high-performance RNA biosensing via electrical interactions |
| title_full | Advancing COVID-19 detection: high-performance RNA biosensing via electrical interactions |
| title_fullStr | Advancing COVID-19 detection: high-performance RNA biosensing via electrical interactions |
| title_full_unstemmed | Advancing COVID-19 detection: high-performance RNA biosensing via electrical interactions |
| title_short | Advancing COVID-19 detection: high-performance RNA biosensing via electrical interactions |
| title_sort | advancing covid 19 detection high performance rna biosensing via electrical interactions |
| topic | RC705-779 Diseases of the respiratory system TA1-2040 Engineering (General). Civil engineering (General) |
| url | https://eprints.ums.edu.my/id/eprint/41424/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/41424/2/FULL%20TEXT.pdf |
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