Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)
This study aimed to investigate the effects of adding endophytic bacterial isolates and Sargassum sp. algae extract to enhance the growth performance and non-specific immune response of cobia (Rachycentron canadum) fry. The research employed a Completely Randomized Design (CRD) experimental method w...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
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ResearchGate
2024
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/41568/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/41568/2/FULL%20TEXT.pdf |
| _version_ | 1825715889065951232 |
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| author | Fittrie M. Pratiwy Muhammad I. Sumantri Suryadi Saputra Rosidah Walim Lili Iskandar Agung Thallita N. Faddilah Rafidah Othman |
| author_facet | Fittrie M. Pratiwy Muhammad I. Sumantri Suryadi Saputra Rosidah Walim Lili Iskandar Agung Thallita N. Faddilah Rafidah Othman |
| author_sort | Fittrie M. Pratiwy |
| collection | UMS |
| description | This study aimed to investigate the effects of adding endophytic bacterial isolates and Sargassum sp. algae extract to enhance the growth performance and non-specific immune response of cobia (Rachycentron canadum) fry. The research employed a Completely Randomized Design (CRD) experimental method with three treatments and five replications. The treatments consisted of: (A) commercial feed without any additives, (B) commercial feed supplemented with 15 mL of Sargassum sp. extract, and (C) commercial feed supplemented with 15 mL of endophytic bacterial isolates from Sargassum sp. at a concentration of 108 CFU mL-1 . The fish fry were reared for 30 days, followed by a 14-day challenge test with Vibrio alginolyticus bacteria at a density of 107 CFU mL-1 , using the intraperitoneal (IP) injection method. Parameters observed included absolute weight gain, absolute length gain, specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency (FE), total erythrocyte count, total leukocyte count, leukocyte differential, clinical symptoms, survival rate, and water quality. Data on absolute weight gain, absolute length gain, SGR, FCR, FE, total erythrocyte count, total leukocyte count, and survival rate were analyzed using ANOVA with a 95% confidence level, followed by Duncan's post hoc test for significant differences. Leukocyte differentials, clinical symptoms, and water quality data were analyzed descriptively. The main findings revealed that the addition of endophytic bacterial isolates and Sargassum sp. algae extract could enhance the growth and non-specific immune response of kobia fry. Treatment C proved to be the most effective, as evidenced by the increased values of absolute weight gain (35.72 g), absolute length gain (11 cm), SGR (3.17%), FCR (1.19%), FE (84.13%), total erythrocyte count (2.89×106 mm-3 ), total leukocyte count (38.56×103 mm3 ), and leukocyte differential percentages (lymphocytes 82.2%, monocytes 4.4%, neutrophils 8.8%), as well as the fastest recovery time (7 days) after the challenge test, with the highest survival rate of 90.7%. |
| first_indexed | 2024-12-09T00:52:56Z |
| format | Article |
| id | ums.eprints-41568 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-12-09T00:52:56Z |
| publishDate | 2024 |
| publisher | ResearchGate |
| record_format | dspace |
| spelling | ums.eprints-415682024-10-24T04:17:34Z https://eprints.ums.edu.my/id/eprint/41568/ Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766) Fittrie M. Pratiwy Muhammad I. Sumantri Suryadi Saputra Rosidah Walim Lili Iskandar Agung Thallita N. Faddilah Rafidah Othman QR1-502 Microbiology SH20.3-191 Aquaculture This study aimed to investigate the effects of adding endophytic bacterial isolates and Sargassum sp. algae extract to enhance the growth performance and non-specific immune response of cobia (Rachycentron canadum) fry. The research employed a Completely Randomized Design (CRD) experimental method with three treatments and five replications. The treatments consisted of: (A) commercial feed without any additives, (B) commercial feed supplemented with 15 mL of Sargassum sp. extract, and (C) commercial feed supplemented with 15 mL of endophytic bacterial isolates from Sargassum sp. at a concentration of 108 CFU mL-1 . The fish fry were reared for 30 days, followed by a 14-day challenge test with Vibrio alginolyticus bacteria at a density of 107 CFU mL-1 , using the intraperitoneal (IP) injection method. Parameters observed included absolute weight gain, absolute length gain, specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency (FE), total erythrocyte count, total leukocyte count, leukocyte differential, clinical symptoms, survival rate, and water quality. Data on absolute weight gain, absolute length gain, SGR, FCR, FE, total erythrocyte count, total leukocyte count, and survival rate were analyzed using ANOVA with a 95% confidence level, followed by Duncan's post hoc test for significant differences. Leukocyte differentials, clinical symptoms, and water quality data were analyzed descriptively. The main findings revealed that the addition of endophytic bacterial isolates and Sargassum sp. algae extract could enhance the growth and non-specific immune response of kobia fry. Treatment C proved to be the most effective, as evidenced by the increased values of absolute weight gain (35.72 g), absolute length gain (11 cm), SGR (3.17%), FCR (1.19%), FE (84.13%), total erythrocyte count (2.89×106 mm-3 ), total leukocyte count (38.56×103 mm3 ), and leukocyte differential percentages (lymphocytes 82.2%, monocytes 4.4%, neutrophils 8.8%), as well as the fastest recovery time (7 days) after the challenge test, with the highest survival rate of 90.7%. ResearchGate 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/41568/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/41568/2/FULL%20TEXT.pdf Fittrie M. Pratiwy and Muhammad I. Sumantri and Suryadi Saputra and Rosidah and Walim Lili and Iskandar Agung and Thallita N. Faddilah and Rafidah Othman (2024) Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766). AACL Bioflux, 17 (4). pp. 1-18. ISSN 1844-8143 |
| spellingShingle | QR1-502 Microbiology SH20.3-191 Aquaculture Fittrie M. Pratiwy Muhammad I. Sumantri Suryadi Saputra Rosidah Walim Lili Iskandar Agung Thallita N. Faddilah Rafidah Othman Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766) |
| title | Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766) |
| title_full | Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766) |
| title_fullStr | Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766) |
| title_full_unstemmed | Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766) |
| title_short | Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766) |
| title_sort | exploring the potential of endophytic bacteria isolated from sargassum sp enhancing growth and non specific immune response of cobia rachycentron canadum linnaeus 1766 |
| topic | QR1-502 Microbiology SH20.3-191 Aquaculture |
| url | https://eprints.ums.edu.my/id/eprint/41568/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/41568/2/FULL%20TEXT.pdf |
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