Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement
This research aimed to determine and analyze the microbial population in kombucha pellicles derived from Sabah black tea, specifically focusing on bacteria and yeast, to gain insights into their abundance, diversity, and potential as prebiotic and probiotic supplements. Despite the growing interest...
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| Format: | Article |
| Language: | English English |
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Universiti Putra Malaysia
2024
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| Online Access: | https://eprints.ums.edu.my/id/eprint/42101/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/42101/2/FULL%20TEXT.pdf |
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| author | Nurul Farhana Nasir Nurul Elyani Mohamad Chee Wei Yew Suryani Saallah Noorjahan Banu Alitheen |
| author_facet | Nurul Farhana Nasir Nurul Elyani Mohamad Chee Wei Yew Suryani Saallah Noorjahan Banu Alitheen |
| author_sort | Nurul Farhana Nasir |
| collection | UMS |
| description | This research aimed to determine and analyze the microbial population in kombucha pellicles derived from Sabah black tea, specifically focusing on bacteria and yeast, to gain insights into their abundance, diversity, and potential as prebiotic and probiotic supplements. Despite the growing interest in kombucha in Malaysia, the specific microbial composition of the pellicle from locally sourced Sabah black teas remains underexplored. Understanding this composition could reveal its potential as a sustainable source of health-promoting microbes. It is hypothesized that Sabah tea kombucha pellicle harbours a beneficial microbial population that can be utilized as a low-cost prebiotic and probiotic supplements. The kombucha pellicle was prepared using 10 g of Sabah black tea, 1 L of sterile water containing 10% sugar (w/v), and a 10% kombucha symbiotic culture of bacteria and yeast (SCOBY). The process included boiling black tea with sugar, adding SCOBY culture, and allowing fermentation for 30 days to obtain cellulosic pellicles. After fermentation, the pellicle was separated, homogenized, and stored for further use. Then, kombucha pellicle genomic DNA was extracted and subjected to 16S and ITS metagenomic analysis to identify the bacteria and fungi population. The 16S and ITS metagenomic results showed that Sabah tea kombucha pellicle contains a potentially beneficial microbial population, mainly Komagataeibacter, Zygosaccharomyces and Starmerella, that may serve as a sustainable probiotic. This current study provides promising evidence for using Sabah tea kombucha pellicle as a low-cost prebiotic and probiotic supplement. This will indirectly help advertise and commercialize Sabah tea as one of the local products in Sabah. |
| first_indexed | 2024-12-09T00:54:23Z |
| format | Article |
| id | ums.eprints-42101 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-12-09T00:54:23Z |
| publishDate | 2024 |
| publisher | Universiti Putra Malaysia |
| record_format | dspace |
| spelling | ums.eprints-421012024-12-04T07:34:41Z https://eprints.ums.edu.my/id/eprint/42101/ Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement Nurul Farhana Nasir Nurul Elyani Mohamad Chee Wei Yew Suryani Saallah Noorjahan Banu Alitheen QL461-599.82 Insects QR75-99.5 Bacteria This research aimed to determine and analyze the microbial population in kombucha pellicles derived from Sabah black tea, specifically focusing on bacteria and yeast, to gain insights into their abundance, diversity, and potential as prebiotic and probiotic supplements. Despite the growing interest in kombucha in Malaysia, the specific microbial composition of the pellicle from locally sourced Sabah black teas remains underexplored. Understanding this composition could reveal its potential as a sustainable source of health-promoting microbes. It is hypothesized that Sabah tea kombucha pellicle harbours a beneficial microbial population that can be utilized as a low-cost prebiotic and probiotic supplements. The kombucha pellicle was prepared using 10 g of Sabah black tea, 1 L of sterile water containing 10% sugar (w/v), and a 10% kombucha symbiotic culture of bacteria and yeast (SCOBY). The process included boiling black tea with sugar, adding SCOBY culture, and allowing fermentation for 30 days to obtain cellulosic pellicles. After fermentation, the pellicle was separated, homogenized, and stored for further use. Then, kombucha pellicle genomic DNA was extracted and subjected to 16S and ITS metagenomic analysis to identify the bacteria and fungi population. The 16S and ITS metagenomic results showed that Sabah tea kombucha pellicle contains a potentially beneficial microbial population, mainly Komagataeibacter, Zygosaccharomyces and Starmerella, that may serve as a sustainable probiotic. This current study provides promising evidence for using Sabah tea kombucha pellicle as a low-cost prebiotic and probiotic supplement. This will indirectly help advertise and commercialize Sabah tea as one of the local products in Sabah. Universiti Putra Malaysia 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42101/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/42101/2/FULL%20TEXT.pdf Nurul Farhana Nasir and Nurul Elyani Mohamad and Chee Wei Yew and Suryani Saallah and Noorjahan Banu Alitheen (2024) Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement. Asia-Pacific Journal of Molecular Biology and Biotechnology, 32 (3). pp. 1-9. ISSN 0128-7451 http://dx.doi.org/10.35118/apjmbb.2024.032.3.24 |
| spellingShingle | QL461-599.82 Insects QR75-99.5 Bacteria Nurul Farhana Nasir Nurul Elyani Mohamad Chee Wei Yew Suryani Saallah Noorjahan Banu Alitheen Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement |
| title | Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement |
| title_full | Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement |
| title_fullStr | Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement |
| title_full_unstemmed | Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement |
| title_short | Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement |
| title_sort | screening of microbial population in sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement |
| topic | QL461-599.82 Insects QR75-99.5 Bacteria |
| url | https://eprints.ums.edu.my/id/eprint/42101/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/42101/2/FULL%20TEXT.pdf |
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