Antioxidant and antihyperglycemic activities of Lygodium microphyllum against alloxan-induced diabetic rats
Diabetes mellitus is the most common endocrine disorder that affects more than 415 million people worldwide. Lygodium microphyllum or better known as Old World Climbing Fern from the family Lygodiaceae, is an invasive climbing fern species that is distributed around Sabah Borneo and possesses many m...
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| Format: | Thesis |
| Language: | English English |
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2019
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| Online Access: | https://eprints.ums.edu.my/id/eprint/42797/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/42797/2/FULLTEXT.pdf |
| _version_ | 1825716098219114496 |
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| author | Dg Syahidah Nadiah Abdull Majid |
| author_facet | Dg Syahidah Nadiah Abdull Majid |
| author_sort | Dg Syahidah Nadiah Abdull Majid |
| collection | UMS |
| description | Diabetes mellitus is the most common endocrine disorder that affects more than 415 million people worldwide. Lygodium microphyllum or better known as Old World Climbing Fern from the family Lygodiaceae, is an invasive climbing fern species that is distributed around Sabah Borneo and possesses many medicinal values in treating several diseases and for health care maintenance. The present study was designed to evaluate the antihyperglycemic potential of L. microphyllum leaves extract against alloxan induced diabetes in rats. In vitro studies have shown that the extract possessed strong antioxidant activity and has ability to scavenge DPPH free radicals effectively. Phytochemical studies revealed the presence of pharmaceutically active compounds of flavonoids, alkaloids, tannins, and steroids. Total phenolic content of leaves was found to be 966.7 ± 0.03 mg/100g dried sample. The total flavonoid content of leaves was found to be 42.9 ± 0.01 mg/100g dried sample. Besides that, experiments designed for antihyperglycemic activity of L. microphyllum against alloxan induced diabetes in rats. Body weight and blood glucose level were measured on interval of 14 days. Thirty-six Sprague Dawley rats were taken and randomly divided into six groups (n=6). Injection of alloxan (i.v.) at a dose of 100 mg/kg body weight in rats resulted in a significant increase in serum glucose as compared to saline treated control. Also, oxidative stress was noticed in pancreatic tissue as evidenced by a significant decrease in glutathione level, glutathione reductase, glutathione-s-transferase, catalase activities, also a significant increase in malondialdehyde when compared to normal saline treated control group. Pancreases were also examined histopathologically by haematoxylin and eosin staining. Additionally, serum biochemistry and oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with L. microphyllum at a dose level of 100, 200 and 400 mg/kg body weight leaves extract for 14 days significantly prevented these alterations and attenuated alloxan-induced oxidative stress. Therefore, the aqueous extract of L. microphyllum may be a good source of natural antioxidant. The results of the present study indicates that the antihyperglycemic potentials of L. microphyllum might be ascribable to its antioxidant and free radical scavenging properties. Thus, it concluded that L. microphyllum may be helpful in the prevention of diabetic complications associated with oxidative stress. |
| first_indexed | 2025-03-05T01:35:51Z |
| format | Thesis |
| id | ums.eprints-42797 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2025-03-05T01:35:51Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ums.eprints-427972025-02-13T06:41:16Z https://eprints.ums.edu.my/id/eprint/42797/ Antioxidant and antihyperglycemic activities of Lygodium microphyllum against alloxan-induced diabetic rats Dg Syahidah Nadiah Abdull Majid RS153-441 Materia medica Diabetes mellitus is the most common endocrine disorder that affects more than 415 million people worldwide. Lygodium microphyllum or better known as Old World Climbing Fern from the family Lygodiaceae, is an invasive climbing fern species that is distributed around Sabah Borneo and possesses many medicinal values in treating several diseases and for health care maintenance. The present study was designed to evaluate the antihyperglycemic potential of L. microphyllum leaves extract against alloxan induced diabetes in rats. In vitro studies have shown that the extract possessed strong antioxidant activity and has ability to scavenge DPPH free radicals effectively. Phytochemical studies revealed the presence of pharmaceutically active compounds of flavonoids, alkaloids, tannins, and steroids. Total phenolic content of leaves was found to be 966.7 ± 0.03 mg/100g dried sample. The total flavonoid content of leaves was found to be 42.9 ± 0.01 mg/100g dried sample. Besides that, experiments designed for antihyperglycemic activity of L. microphyllum against alloxan induced diabetes in rats. Body weight and blood glucose level were measured on interval of 14 days. Thirty-six Sprague Dawley rats were taken and randomly divided into six groups (n=6). Injection of alloxan (i.v.) at a dose of 100 mg/kg body weight in rats resulted in a significant increase in serum glucose as compared to saline treated control. Also, oxidative stress was noticed in pancreatic tissue as evidenced by a significant decrease in glutathione level, glutathione reductase, glutathione-s-transferase, catalase activities, also a significant increase in malondialdehyde when compared to normal saline treated control group. Pancreases were also examined histopathologically by haematoxylin and eosin staining. Additionally, serum biochemistry and oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with L. microphyllum at a dose level of 100, 200 and 400 mg/kg body weight leaves extract for 14 days significantly prevented these alterations and attenuated alloxan-induced oxidative stress. Therefore, the aqueous extract of L. microphyllum may be a good source of natural antioxidant. The results of the present study indicates that the antihyperglycemic potentials of L. microphyllum might be ascribable to its antioxidant and free radical scavenging properties. Thus, it concluded that L. microphyllum may be helpful in the prevention of diabetic complications associated with oxidative stress. 2019 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42797/1/24%20PAGES.pdf text en https://eprints.ums.edu.my/id/eprint/42797/2/FULLTEXT.pdf Dg Syahidah Nadiah Abdull Majid (2019) Antioxidant and antihyperglycemic activities of Lygodium microphyllum against alloxan-induced diabetic rats. Masters thesis, Universiti Malaysia Sabah. |
| spellingShingle | RS153-441 Materia medica Dg Syahidah Nadiah Abdull Majid Antioxidant and antihyperglycemic activities of Lygodium microphyllum against alloxan-induced diabetic rats |
| title | Antioxidant and antihyperglycemic activities of Lygodium microphyllum against alloxan-induced diabetic rats |
| title_full | Antioxidant and antihyperglycemic activities of Lygodium microphyllum against alloxan-induced diabetic rats |
| title_fullStr | Antioxidant and antihyperglycemic activities of Lygodium microphyllum against alloxan-induced diabetic rats |
| title_full_unstemmed | Antioxidant and antihyperglycemic activities of Lygodium microphyllum against alloxan-induced diabetic rats |
| title_short | Antioxidant and antihyperglycemic activities of Lygodium microphyllum against alloxan-induced diabetic rats |
| title_sort | antioxidant and antihyperglycemic activities of lygodium microphyllum against alloxan induced diabetic rats |
| topic | RS153-441 Materia medica |
| url | https://eprints.ums.edu.my/id/eprint/42797/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/42797/2/FULLTEXT.pdf |
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