Modulatory effects of Clidemia hirta against carbon tetrachloride (CCI₄) induced fulminant hepatic failure and necrosis in mice
Liver diseases still represent a major health burden worldwide. Moreover, medicinal plants have gained popularity in the treatment of several diseases including liver. Clidemia hirta possesses many medicinal properties in healing several diseases and for health care maintenance. However, hepatoprote...
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| Format: | Thesis |
| Language: | English English |
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2017
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| Online Access: | https://eprints.ums.edu.my/id/eprint/42798/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/42798/2/FULLTEXT.pdf |
| _version_ | 1825716098408906752 |
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| author | Nurul Amzar |
| author_facet | Nurul Amzar |
| author_sort | Nurul Amzar |
| collection | UMS |
| description | Liver diseases still represent a major health burden worldwide. Moreover, medicinal plants have gained popularity in the treatment of several diseases including liver. Clidemia hirta possesses many medicinal properties in healing several diseases and for health care maintenance. However, hepatoprotective effect and antioxidative potential of C. hirta has not fully investigated. Thus, the present study was to evaluate the hepatoprotective and antioxidative potential of aqueous extract of C. hirta leaves against carbon tetrachloride (CCl4)-induced liver injuries and oxidative damage in mice. Various biochemical changes associated with liver damage and oxidative stress were measured. Phytochemical screening showed the presence of saponin, flavonoid, steroid, tannins and cardiac glycosides of C. hirta. Total phenolic content was 610.24 mg/g GAE and flavonoid was 91.67 mg/g CAE. The DPPH free radical scavenging activity showed inhibition of 94.62% at 620 μg/ml and inhibition concentration (IC50) was 45.48 μg/ml for C. hirta. For in vivo studies, the mice were pre-treated for 14 consecutive days with aqueous extract of C. hirta (150 mg/kg body weight, 300 mg/kg body weight and 600 mg/kg body weight) followed by two dosages of CCl4 (1.0 ml/kg body weight) orally on day 14 and 15. All of these animals were sacrificed 24 hours after the last dose of CCl4 or saline. Blood and liver tissues were taken quickly for biochemical and histopathological studies to assess the derangement in the functioning of liver. The development of the oxidative stress was observed through the escalation of hepatic lipid peroxidation, depletion of reduced glutathione and antioxidant enzymes (glutathione peroxidase, glutathione reductase, catalase, glutathione S-transferase and quinone reductase). Hepatic damage was evaluated by measuring serum transaminase (ALT and AST). In addition, CCl4- mediated hepatic damage was further evaluated by histopathological examination. However, most of these changes were ameliorated by pretreatment of mice with C. hirta in a dose dependent manner. Biochemical improvements after C. hirta treatment were paralleled by histopathological findings. The results of the present study indicated that hepatoprotective effect of aqueous extract of C. hirta might be ascribable to its antioxidant and free radical scavenging properties. |
| first_indexed | 2025-03-05T01:35:52Z |
| format | Thesis |
| id | ums.eprints-42798 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2025-03-05T01:35:52Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ums.eprints-427982025-02-13T06:41:22Z https://eprints.ums.edu.my/id/eprint/42798/ Modulatory effects of Clidemia hirta against carbon tetrachloride (CCI₄) induced fulminant hepatic failure and necrosis in mice Nurul Amzar QK710-899 Plant physiology Liver diseases still represent a major health burden worldwide. Moreover, medicinal plants have gained popularity in the treatment of several diseases including liver. Clidemia hirta possesses many medicinal properties in healing several diseases and for health care maintenance. However, hepatoprotective effect and antioxidative potential of C. hirta has not fully investigated. Thus, the present study was to evaluate the hepatoprotective and antioxidative potential of aqueous extract of C. hirta leaves against carbon tetrachloride (CCl4)-induced liver injuries and oxidative damage in mice. Various biochemical changes associated with liver damage and oxidative stress were measured. Phytochemical screening showed the presence of saponin, flavonoid, steroid, tannins and cardiac glycosides of C. hirta. Total phenolic content was 610.24 mg/g GAE and flavonoid was 91.67 mg/g CAE. The DPPH free radical scavenging activity showed inhibition of 94.62% at 620 μg/ml and inhibition concentration (IC50) was 45.48 μg/ml for C. hirta. For in vivo studies, the mice were pre-treated for 14 consecutive days with aqueous extract of C. hirta (150 mg/kg body weight, 300 mg/kg body weight and 600 mg/kg body weight) followed by two dosages of CCl4 (1.0 ml/kg body weight) orally on day 14 and 15. All of these animals were sacrificed 24 hours after the last dose of CCl4 or saline. Blood and liver tissues were taken quickly for biochemical and histopathological studies to assess the derangement in the functioning of liver. The development of the oxidative stress was observed through the escalation of hepatic lipid peroxidation, depletion of reduced glutathione and antioxidant enzymes (glutathione peroxidase, glutathione reductase, catalase, glutathione S-transferase and quinone reductase). Hepatic damage was evaluated by measuring serum transaminase (ALT and AST). In addition, CCl4- mediated hepatic damage was further evaluated by histopathological examination. However, most of these changes were ameliorated by pretreatment of mice with C. hirta in a dose dependent manner. Biochemical improvements after C. hirta treatment were paralleled by histopathological findings. The results of the present study indicated that hepatoprotective effect of aqueous extract of C. hirta might be ascribable to its antioxidant and free radical scavenging properties. 2017 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42798/1/24%20PAGES.pdf text en https://eprints.ums.edu.my/id/eprint/42798/2/FULLTEXT.pdf Nurul Amzar (2017) Modulatory effects of Clidemia hirta against carbon tetrachloride (CCI₄) induced fulminant hepatic failure and necrosis in mice. Masters thesis, Universiti Malaysia Sabah. |
| spellingShingle | QK710-899 Plant physiology Nurul Amzar Modulatory effects of Clidemia hirta against carbon tetrachloride (CCI₄) induced fulminant hepatic failure and necrosis in mice |
| title | Modulatory effects of Clidemia hirta against carbon tetrachloride (CCI₄) induced fulminant hepatic failure and necrosis in mice |
| title_full | Modulatory effects of Clidemia hirta against carbon tetrachloride (CCI₄) induced fulminant hepatic failure and necrosis in mice |
| title_fullStr | Modulatory effects of Clidemia hirta against carbon tetrachloride (CCI₄) induced fulminant hepatic failure and necrosis in mice |
| title_full_unstemmed | Modulatory effects of Clidemia hirta against carbon tetrachloride (CCI₄) induced fulminant hepatic failure and necrosis in mice |
| title_short | Modulatory effects of Clidemia hirta against carbon tetrachloride (CCI₄) induced fulminant hepatic failure and necrosis in mice |
| title_sort | modulatory effects of clidemia hirta against carbon tetrachloride cci₄ induced fulminant hepatic failure and necrosis in mice |
| topic | QK710-899 Plant physiology |
| url | https://eprints.ums.edu.my/id/eprint/42798/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/42798/2/FULLTEXT.pdf |
| work_keys_str_mv | AT nurulamzar modulatoryeffectsofclidemiahirtaagainstcarbontetrachloridecci4inducedfulminanthepaticfailureandnecrosisinmice |