Biocompatibility study of curcumin-loaded Pluronic F127 nanoformulation (NanoCUR) against the embryonic development of zebrafish (Danio rerio)
Curcumin (CUR) has been studied for its biomedical applications due to its active biological properties. However, CUR has limitations such as poor solubility, low bioavailability, and rapid degradation. Thus, CUR was nanoformulated with the application of polymeric micelle. Previous studies of CUR-l...
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| Format: | Article |
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MDPI
2022
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| _version_ | 1796983912908455936 |
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| author | Abdullah, Siti Nur Sharmila Subramaniam, Kalai Arasu Muhamad Zamani, Zahir Haizat Sarchio, Seri Narti Edayu Md Yasin, Faizah Shamsi, Suhaili |
| author_facet | Abdullah, Siti Nur Sharmila Subramaniam, Kalai Arasu Muhamad Zamani, Zahir Haizat Sarchio, Seri Narti Edayu Md Yasin, Faizah Shamsi, Suhaili |
| author_sort | Abdullah, Siti Nur Sharmila |
| collection | UPM |
| description | Curcumin (CUR) has been studied for its biomedical applications due to its active biological properties. However, CUR has limitations such as poor solubility, low bioavailability, and rapid degradation. Thus, CUR was nanoformulated with the application of polymeric micelle. Previous studies of CUR-loaded Pluronic F127 nanoformulation (NanoCUR) were generally prioritized toward cancer cells and its therapeutic values. There are reports that emphasize the toxicity of CUR, but reports on the toxicity of NanoCUR on embryonic developmental stages is still scarce. The present study aims to investigate the toxicity effects of NanoCUR on the embryonic development of zebrafish (Danio rerio). NanoCUR was synthesized via thin film hydration method and then characterized using DLS, UV-Vis, FTIR, FESEM, and XRD. The toxicity assessment of NanoCUR was conducted using zebrafish embryos, in comparison to native CUR, as well as Pluronic F127 (PF) as the controls, and ROS assay was further carried out. It was revealed that NanoCUR showed an improved toxicity profile compared to native CUR. NanoCUR displayed a delayed toxicity response and showed a concentration- and time-dependent toxicity response. NanoCUR was also observed to generate a significantly low reactive oxygen species (ROS) compared to native CUR in ROS assay. Overall, the results obtained highlight the potential of NanoCUR to be developed in clinical settings due to its improved toxicity profile compared to CUR. |
| first_indexed | 2024-03-06T11:13:07Z |
| format | Article |
| id | upm.eprints-100536 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:13:07Z |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-1005362023-11-21T09:05:51Z http://psasir.upm.edu.my/id/eprint/100536/ Biocompatibility study of curcumin-loaded Pluronic F127 nanoformulation (NanoCUR) against the embryonic development of zebrafish (Danio rerio) Abdullah, Siti Nur Sharmila Subramaniam, Kalai Arasu Muhamad Zamani, Zahir Haizat Sarchio, Seri Narti Edayu Md Yasin, Faizah Shamsi, Suhaili Curcumin (CUR) has been studied for its biomedical applications due to its active biological properties. However, CUR has limitations such as poor solubility, low bioavailability, and rapid degradation. Thus, CUR was nanoformulated with the application of polymeric micelle. Previous studies of CUR-loaded Pluronic F127 nanoformulation (NanoCUR) were generally prioritized toward cancer cells and its therapeutic values. There are reports that emphasize the toxicity of CUR, but reports on the toxicity of NanoCUR on embryonic developmental stages is still scarce. The present study aims to investigate the toxicity effects of NanoCUR on the embryonic development of zebrafish (Danio rerio). NanoCUR was synthesized via thin film hydration method and then characterized using DLS, UV-Vis, FTIR, FESEM, and XRD. The toxicity assessment of NanoCUR was conducted using zebrafish embryos, in comparison to native CUR, as well as Pluronic F127 (PF) as the controls, and ROS assay was further carried out. It was revealed that NanoCUR showed an improved toxicity profile compared to native CUR. NanoCUR displayed a delayed toxicity response and showed a concentration- and time-dependent toxicity response. NanoCUR was also observed to generate a significantly low reactive oxygen species (ROS) compared to native CUR in ROS assay. Overall, the results obtained highlight the potential of NanoCUR to be developed in clinical settings due to its improved toxicity profile compared to CUR. MDPI 2022-07-14 Article PeerReviewed Abdullah, Siti Nur Sharmila and Subramaniam, Kalai Arasu and Muhamad Zamani, Zahir Haizat and Sarchio, Seri Narti Edayu and Md Yasin, Faizah and Shamsi, Suhaili (2022) Biocompatibility study of curcumin-loaded Pluronic F127 nanoformulation (NanoCUR) against the embryonic development of zebrafish (Danio rerio). Molecules, 27 (14). art. no. 4493. pp. 1-17. ISSN 1420-3049 https://www.mdpi.com/1420-3049/27/14/4493 10.3390/molecules27144493 |
| spellingShingle | Abdullah, Siti Nur Sharmila Subramaniam, Kalai Arasu Muhamad Zamani, Zahir Haizat Sarchio, Seri Narti Edayu Md Yasin, Faizah Shamsi, Suhaili Biocompatibility study of curcumin-loaded Pluronic F127 nanoformulation (NanoCUR) against the embryonic development of zebrafish (Danio rerio) |
| title | Biocompatibility study of curcumin-loaded Pluronic F127 nanoformulation (NanoCUR) against the embryonic development of zebrafish (Danio rerio) |
| title_full | Biocompatibility study of curcumin-loaded Pluronic F127 nanoformulation (NanoCUR) against the embryonic development of zebrafish (Danio rerio) |
| title_fullStr | Biocompatibility study of curcumin-loaded Pluronic F127 nanoformulation (NanoCUR) against the embryonic development of zebrafish (Danio rerio) |
| title_full_unstemmed | Biocompatibility study of curcumin-loaded Pluronic F127 nanoformulation (NanoCUR) against the embryonic development of zebrafish (Danio rerio) |
| title_short | Biocompatibility study of curcumin-loaded Pluronic F127 nanoformulation (NanoCUR) against the embryonic development of zebrafish (Danio rerio) |
| title_sort | biocompatibility study of curcumin loaded pluronic f127 nanoformulation nanocur against the embryonic development of zebrafish danio rerio |
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