Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens
Plant cell culture technology serves as an effective alternative system for in vitro production of bioactive molecules, as it allows for the exploration of valuable compounds under a controlled environment. The present study was conducted to evaluate the effect of plant growth regulator (PGR); 2,4-d...
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| Format: | Article |
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Sains Malaysiana
2022
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| _version_ | 1796983950450622464 |
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| author | Yaacob, Jamilah Syafawati Ramli, Muhammad Aiman Abd Rahim, Muhamad Hafiz Selvaraj, Abigail Marie Robert Nyanasaigran, Laavanya |
| author_facet | Yaacob, Jamilah Syafawati Ramli, Muhammad Aiman Abd Rahim, Muhamad Hafiz Selvaraj, Abigail Marie Robert Nyanasaigran, Laavanya |
| author_sort | Yaacob, Jamilah Syafawati |
| collection | UPM |
| description | Plant cell culture technology serves as an effective alternative system for in vitro production of bioactive molecules, as it allows for the exploration of valuable compounds under a controlled environment. The present study was conducted to evaluate the effect of plant growth regulator (PGR); 2,4-dichlorophenoxyacetic acid (2,4-D) on the expression of compounds in coloured callus of Capsicum frutescens, a vital spice in various cuisines worldwide. The differential accumulation of compounds in the callus was analysed using liquid chromatography-mass spectrometry (LCMS) and the PGR concentration that resulted in the highest accumulation of the valuable compounds was identified. In this study, calli of various colours (cream, yellow and green) were successfully produced from C. frutescens through plant tissue culture. The increase in 2,4-D concentrations was found to increase callus growth index (GI) and specific growth rate (Sg), where the highest GI (0.5690) and Sg (0.6348 mg/week) were observed in callus produced in media supplemented with 0.5 mg/L 2,4-D. LCMS data analyses showed that 19 compounds were detected in the callus, with 8 compounds (fatty acids and phenolics) were successfully identified, while the remaining 11 compounds were reported as unknowns. Yellow-coloured callus was observed to contain the highest number of compounds (18 compounds), while green callus contained the least (14 compounds). This analysis provides valuable information on the application of biotechnological tools such as plant tissue culture as an alternative for sustainable production of compounds with high bioactivity in Capsicum frutescens. |
| first_indexed | 2024-03-06T11:13:41Z |
| format | Article |
| id | upm.eprints-100736 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:13:41Z |
| publishDate | 2022 |
| publisher | Sains Malaysiana |
| record_format | dspace |
| spelling | upm.eprints-1007362023-09-11T08:01:00Z http://psasir.upm.edu.my/id/eprint/100736/ Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens Yaacob, Jamilah Syafawati Ramli, Muhammad Aiman Abd Rahim, Muhamad Hafiz Selvaraj, Abigail Marie Robert Nyanasaigran, Laavanya Plant cell culture technology serves as an effective alternative system for in vitro production of bioactive molecules, as it allows for the exploration of valuable compounds under a controlled environment. The present study was conducted to evaluate the effect of plant growth regulator (PGR); 2,4-dichlorophenoxyacetic acid (2,4-D) on the expression of compounds in coloured callus of Capsicum frutescens, a vital spice in various cuisines worldwide. The differential accumulation of compounds in the callus was analysed using liquid chromatography-mass spectrometry (LCMS) and the PGR concentration that resulted in the highest accumulation of the valuable compounds was identified. In this study, calli of various colours (cream, yellow and green) were successfully produced from C. frutescens through plant tissue culture. The increase in 2,4-D concentrations was found to increase callus growth index (GI) and specific growth rate (Sg), where the highest GI (0.5690) and Sg (0.6348 mg/week) were observed in callus produced in media supplemented with 0.5 mg/L 2,4-D. LCMS data analyses showed that 19 compounds were detected in the callus, with 8 compounds (fatty acids and phenolics) were successfully identified, while the remaining 11 compounds were reported as unknowns. Yellow-coloured callus was observed to contain the highest number of compounds (18 compounds), while green callus contained the least (14 compounds). This analysis provides valuable information on the application of biotechnological tools such as plant tissue culture as an alternative for sustainable production of compounds with high bioactivity in Capsicum frutescens. Sains Malaysiana 2022-10 Article PeerReviewed Yaacob, Jamilah Syafawati and Ramli, Muhammad Aiman and Abd Rahim, Muhamad Hafiz and Selvaraj, Abigail Marie Robert and Nyanasaigran, Laavanya (2022) Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens. Penerbit Universiti Kebangsaan Malaysia, 51 (10). 3171 - 3182. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol51num10_2022/vol51num10_2022pg3171-3182.html 10.17576/jsm-2022-5110-05 |
| spellingShingle | Yaacob, Jamilah Syafawati Ramli, Muhammad Aiman Abd Rahim, Muhamad Hafiz Selvaraj, Abigail Marie Robert Nyanasaigran, Laavanya Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens |
| title | Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens |
| title_full | Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens |
| title_fullStr | Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens |
| title_full_unstemmed | Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens |
| title_short | Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens |
| title_sort | comparative analysis on the role of 2 4 dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of capsicum frutescens |
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