Consolidated bioethanol production using Trichoderma asperellum B1581

Consolidated bioprocessing (CBP) is an alternative commercial process that combines all essential processes in a single bioreactor for the conversion of lignocellulosic biomass into ethanol. The challenge in the development of CBP is to find microorganisms with crucial properties for the utilisation...

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Main Authors: Mohamed Ghazali, Mona Fatin Syazwanee, Mustafa, Muskhazli, Mohd Zainudin, Nur Ain Izzati, Abd Aziz, Nor Azwady
Format: Article
Published: The Deanship of Scientific Research , The Hashemite University, Jordan 2022
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author Mohamed Ghazali, Mona Fatin Syazwanee
Mustafa, Muskhazli
Mohd Zainudin, Nur Ain Izzati
Abd Aziz, Nor Azwady
author_facet Mohamed Ghazali, Mona Fatin Syazwanee
Mustafa, Muskhazli
Mohd Zainudin, Nur Ain Izzati
Abd Aziz, Nor Azwady
author_sort Mohamed Ghazali, Mona Fatin Syazwanee
collection UPM
description Consolidated bioprocessing (CBP) is an alternative commercial process that combines all essential processes in a single bioreactor for the conversion of lignocellulosic biomass into ethanol. The challenge in the development of CBP is to find microorganisms with crucial properties for the utilisation of lignocellulosic materials to produce bioethanol. Also, it can be difficult to determine the optimal culture conditions for all processes to occur simultaneously. Therefore, this study focused on the potential of Trichoderma asperellum B1581 to produce ethanol and optimised the physicochemical parameters required for paddy straw waste conversion via CBP. Six parameters (days of saccharification, saccharification temperature (°C), days of fermentation, fermentation temperature (°C), medium level (%, v/v), and substrates loading (%, w/v)) were optimised in one-factor-at-a-time (OFAT) analysis via Response Surface Methodology (RSM). The numerical optimisation was statistically validated by comparing the volume of ethanol produced to the volume predicted by the RSM. T. asperellum B1581 produced 0.94 g/L bioethanol in CBP and is a more convenient, manageable and cost-effective process as all the crucial steps were performed by only one organism.
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spelling upm.eprints-1007882023-08-22T03:57:17Z http://psasir.upm.edu.my/id/eprint/100788/ Consolidated bioethanol production using Trichoderma asperellum B1581 Mohamed Ghazali, Mona Fatin Syazwanee Mustafa, Muskhazli Mohd Zainudin, Nur Ain Izzati Abd Aziz, Nor Azwady Consolidated bioprocessing (CBP) is an alternative commercial process that combines all essential processes in a single bioreactor for the conversion of lignocellulosic biomass into ethanol. The challenge in the development of CBP is to find microorganisms with crucial properties for the utilisation of lignocellulosic materials to produce bioethanol. Also, it can be difficult to determine the optimal culture conditions for all processes to occur simultaneously. Therefore, this study focused on the potential of Trichoderma asperellum B1581 to produce ethanol and optimised the physicochemical parameters required for paddy straw waste conversion via CBP. Six parameters (days of saccharification, saccharification temperature (°C), days of fermentation, fermentation temperature (°C), medium level (%, v/v), and substrates loading (%, w/v)) were optimised in one-factor-at-a-time (OFAT) analysis via Response Surface Methodology (RSM). The numerical optimisation was statistically validated by comparing the volume of ethanol produced to the volume predicted by the RSM. T. asperellum B1581 produced 0.94 g/L bioethanol in CBP and is a more convenient, manageable and cost-effective process as all the crucial steps were performed by only one organism. The Deanship of Scientific Research , The Hashemite University, Jordan 2022-12 Article PeerReviewed Mohamed Ghazali, Mona Fatin Syazwanee and Mustafa, Muskhazli and Mohd Zainudin, Nur Ain Izzati and Abd Aziz, Nor Azwady (2022) Consolidated bioethanol production using Trichoderma asperellum B1581. Jordan Journal of Biological Sciences, 15 (4). pp. 621-627. ISSN 1995-6673 https://jjbs.hu.edu.jo/vol15.htm 10.54319/jjbs/150410
spellingShingle Mohamed Ghazali, Mona Fatin Syazwanee
Mustafa, Muskhazli
Mohd Zainudin, Nur Ain Izzati
Abd Aziz, Nor Azwady
Consolidated bioethanol production using Trichoderma asperellum B1581
title Consolidated bioethanol production using Trichoderma asperellum B1581
title_full Consolidated bioethanol production using Trichoderma asperellum B1581
title_fullStr Consolidated bioethanol production using Trichoderma asperellum B1581
title_full_unstemmed Consolidated bioethanol production using Trichoderma asperellum B1581
title_short Consolidated bioethanol production using Trichoderma asperellum B1581
title_sort consolidated bioethanol production using trichoderma asperellum b1581
work_keys_str_mv AT mohamedghazalimonafatinsyazwanee consolidatedbioethanolproductionusingtrichodermaasperellumb1581
AT mustafamuskhazli consolidatedbioethanolproductionusingtrichodermaasperellumb1581
AT mohdzainudinnurainizzati consolidatedbioethanolproductionusingtrichodermaasperellumb1581
AT abdaziznorazwady consolidatedbioethanolproductionusingtrichodermaasperellumb1581