Designed mini protein 20 mimicking uricase encapsulated in ZIF-8 as nanozyme biosensor for uric acid detection
This work presents the use of encapsulated mini protein 20 mimicking uricase (mp20)-zeolitic imidazolate framework-8 (ZIF-8) as a bioreceptor for the development of a nanozyme-based electrochemical biosensor for uric acid detection. The electrochemical performance of the biofunctionalized mp20@ZIF-8...
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MDPI
2022
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| author | Abdul Aziz, Siti Fatimah Nur Salleh, Abu Bakar Ashari, Siti Efliza Normi, Yahaya M. Yusof, Nor Azah Ahmad, Shahrul Ainliah Alang |
| author_facet | Abdul Aziz, Siti Fatimah Nur Salleh, Abu Bakar Ashari, Siti Efliza Normi, Yahaya M. Yusof, Nor Azah Ahmad, Shahrul Ainliah Alang |
| author_sort | Abdul Aziz, Siti Fatimah Nur |
| collection | UPM |
| description | This work presents the use of encapsulated mini protein 20 mimicking uricase (mp20)-zeolitic imidazolate framework-8 (ZIF-8) as a bioreceptor for the development of a nanozyme-based electrochemical biosensor for uric acid detection. The electrochemical performance of the biofunctionalized mp20@ZIF-8 on the reduced graphene oxide/screen-printed carbon electrode (rGO/SPCE) was investigated by optimizing operating parameters such as pH, deposition potential, and deposition time using a central composite design-response surface methodology (CCD-RSM). The quadratic regression model was developed to correlate the combination of each variable to the oxidation current density as a response. A significant effect on current response was observed under optimized conditions of pH of 7.4 at −0.35 V deposition potential and 56.56 s deposition time, with p < 0.05 for each interacted factor. The obtained coefficient of determination (R2) value of 0.9992 indicated good agreement with the experimental finding. The developed nanozyme biosensor (mp20@ZIF-8/rGO/SPCE) exhibited high selectivity in the presence of the same fold concentration of interfering species with a detection limit of 0.27 μM, over a concentration range of 1 to 34 μM. The practicality of the tailored biosensor in monitoring uric acid in human serum and urine samples was validated with high-performance liquid chromatography (HPLC) and a commercial uric acid meter. Hence, nanozyme-based is a promising platform that offers a rapid, sensitive, selective, and low-cost biosensor for the non-enzymatic detection of uric acid in biological samples. |
| first_indexed | 2024-03-06T11:14:08Z |
| format | Article |
| id | upm.eprints-100902 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:14:08Z |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-1009022023-07-14T04:03:06Z http://psasir.upm.edu.my/id/eprint/100902/ Designed mini protein 20 mimicking uricase encapsulated in ZIF-8 as nanozyme biosensor for uric acid detection Abdul Aziz, Siti Fatimah Nur Salleh, Abu Bakar Ashari, Siti Efliza Normi, Yahaya M. Yusof, Nor Azah Ahmad, Shahrul Ainliah Alang This work presents the use of encapsulated mini protein 20 mimicking uricase (mp20)-zeolitic imidazolate framework-8 (ZIF-8) as a bioreceptor for the development of a nanozyme-based electrochemical biosensor for uric acid detection. The electrochemical performance of the biofunctionalized mp20@ZIF-8 on the reduced graphene oxide/screen-printed carbon electrode (rGO/SPCE) was investigated by optimizing operating parameters such as pH, deposition potential, and deposition time using a central composite design-response surface methodology (CCD-RSM). The quadratic regression model was developed to correlate the combination of each variable to the oxidation current density as a response. A significant effect on current response was observed under optimized conditions of pH of 7.4 at −0.35 V deposition potential and 56.56 s deposition time, with p < 0.05 for each interacted factor. The obtained coefficient of determination (R2) value of 0.9992 indicated good agreement with the experimental finding. The developed nanozyme biosensor (mp20@ZIF-8/rGO/SPCE) exhibited high selectivity in the presence of the same fold concentration of interfering species with a detection limit of 0.27 μM, over a concentration range of 1 to 34 μM. The practicality of the tailored biosensor in monitoring uric acid in human serum and urine samples was validated with high-performance liquid chromatography (HPLC) and a commercial uric acid meter. Hence, nanozyme-based is a promising platform that offers a rapid, sensitive, selective, and low-cost biosensor for the non-enzymatic detection of uric acid in biological samples. MDPI 2022-07-04 Article PeerReviewed Abdul Aziz, Siti Fatimah Nur and Salleh, Abu Bakar and Ashari, Siti Efliza and Normi, Yahaya M. and Yusof, Nor Azah and Ahmad, Shahrul Ainliah Alang (2022) Designed mini protein 20 mimicking uricase encapsulated in ZIF-8 as nanozyme biosensor for uric acid detection. Nanomaterials, 12 (13). art. no. 2290. pp. 1-16. ISSN 2079-4991 https://www.mdpi.com/2079-4991/12/13/2290 10.3390/nano12132290 |
| spellingShingle | Abdul Aziz, Siti Fatimah Nur Salleh, Abu Bakar Ashari, Siti Efliza Normi, Yahaya M. Yusof, Nor Azah Ahmad, Shahrul Ainliah Alang Designed mini protein 20 mimicking uricase encapsulated in ZIF-8 as nanozyme biosensor for uric acid detection |
| title | Designed mini protein 20 mimicking uricase encapsulated in ZIF-8 as nanozyme biosensor for uric acid detection |
| title_full | Designed mini protein 20 mimicking uricase encapsulated in ZIF-8 as nanozyme biosensor for uric acid detection |
| title_fullStr | Designed mini protein 20 mimicking uricase encapsulated in ZIF-8 as nanozyme biosensor for uric acid detection |
| title_full_unstemmed | Designed mini protein 20 mimicking uricase encapsulated in ZIF-8 as nanozyme biosensor for uric acid detection |
| title_short | Designed mini protein 20 mimicking uricase encapsulated in ZIF-8 as nanozyme biosensor for uric acid detection |
| title_sort | designed mini protein 20 mimicking uricase encapsulated in zif 8 as nanozyme biosensor for uric acid detection |
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