The Effects of Different IBA Concentrations, Light Regimes of in Vitro Rooting and Acclimatization of Invitro Teak (Tectona Grandis L.F) Plantlets
The effect of different IBA concentrations, two light regimes of in vitro rooting and acclimatization on in vitro teak ( Tectona grandis L.t) was investigated. The experiments followed a Completely Randomized Design (CRD) involving six concentrations of IBA (0mg/l, 0.5mg/, 1.0mg/l, 2.0mg/l, 3.0mg...
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| Format: | Project Paper Report |
| Language: | English English |
| Published: |
2003
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10124/1/FH_2003_3.pdf |
| Summary: | The effect of different IBA concentrations, two light regimes of in vitro rooting
and acclimatization on in vitro teak ( Tectona grandis L.t) was investigated. The
experiments followed a Completely Randomized Design (CRD) involving six
concentrations of IBA (0mg/l, 0.5mg/, 1.0mg/l, 2.0mg/l, 3.0mg/l and 5.0mg/l) and two
light regimes with 15 replicates each. The in vitro rooting experiments showed that
shoots cultures in White medium supplemented with 2.0mg/l and 5 .0mgll IBA produced
the best results in terms of mean number of root (6.33=6 and 7.66=8) and mean root
length (1l.82mm and 8mm) respectively. In vitro shootlets incubated in the dark
exhibited earlier root initiation by five days compared to those incubated in the light.
Generally, shoots incubated in the light produced significantly higher mean number of
roots (2.3) and mean root length (15.5 mm and 4.5 mm) when supplemented with low
concentrations of 0.5mg/l and 1.0mg/l IBA. Conversely when shoots were incubated in
the dark, higher mean root length of (14.0mm and 8.8mm) and mean number of root
(7.5=8 and 3.3=3 ) were produced when being supplemented with higher concentrations
of 2.0mg/l and 3 .0mg/l respectively. Chiefly 2.0mg/l IBA was found to be optimum for
rooting of teak as it recorded high mean number of root (6.3=6 and 7.5=8) and mean root
length (11.8mm and 1 4.0mm) regardless of light regime. The higher survival percentage
of 80% was recorded for plantlet grown in "jiffy-7" but only 40 % survived in the
autoclaved soil after one week. However the plantlets failed to continue to survive after
the second week due to unforeseen technical error such as a faulty growth chamber. The
present result substantiates the requirements of IBA to promote the in vitro rooting of
teak shootlets and dark incubation to accelerate rhizogenesis. |
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