Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination
Insecticides are an example of an environmental contaminant that can cause harmful effects on various types of organisms. Implementation of continuous monitoring program is needed to ensure the level of contamination can be controlled. In this present study, acetylcholinesterase (AChE) from the brai...
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| Format: | Article |
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IOP Publishing
2022
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| author | Khalidi, S. A. M. Sabullah,, M. K. Gansau, J. A. Faik, A. A. M. Sani, S. A. Shukor, M. Y. |
| author_facet | Khalidi, S. A. M. Sabullah,, M. K. Gansau, J. A. Faik, A. A. M. Sani, S. A. Shukor, M. Y. |
| author_sort | Khalidi, S. A. M. |
| collection | UPM |
| description | Insecticides are an example of an environmental contaminant that can cause harmful effects on various types of organisms. Implementation of continuous monitoring program is needed to ensure the level of contamination can be controlled. In this present study, acetylcholinesterase (AChE) from the brain of <jats:italic>Monopterus albus</jats:italic> was elucidated to determine the potential alternative source of biosensor kit, which is sensitive towards various insecticides, especially carbamates. AChE from <jats:italic>M. albus</jats:italic> brain was extracted and purified through ammonium sulfate precipitation followed by procainamide-based affinity chromatography. Carbamate insecticides were used, such as bendiocarb, carbaryl, carbofuran, methomyl, and propoxur, to test their ability to inhibit AChE activity. Bendiocarb and methomyl show the capability to inhibit almost half of the enzyme activity at 51.05 and 51.20 %, respectively, while carbaryl, propoxur, and carbofuran inhibit 43.03, 42.80, and 15.06 %, respectively. Bendiocarb and methomyl were selected, and <jats:italic>M. albus</jats:italic> AChE was separately exposed with different concentrations of those carbamates and half maximal inhibitory concentration; IC50 was determined at 0.874 and 1.639 ppm, respectively. A field trial was conducted by testing the enzyme with various vegetable samples. All samples show no significant effect on AChE activity, meaning there was no existence of insecticides in each sample (p > 0.05). This study could be used as an alternative source for developing biosensor kits for the environmental monitoring program. |
| first_indexed | 2024-09-25T03:36:32Z |
| format | Article |
| id | upm.eprints-101291 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:36:32Z |
| publishDate | 2022 |
| publisher | IOP Publishing |
| record_format | dspace |
| spelling | upm.eprints-1012912024-05-02T06:34:49Z http://psasir.upm.edu.my/id/eprint/101291/ Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination Khalidi, S. A. M. Sabullah,, M. K. Gansau, J. A. Faik, A. A. M. Sani, S. A. Shukor, M. Y. Insecticides are an example of an environmental contaminant that can cause harmful effects on various types of organisms. Implementation of continuous monitoring program is needed to ensure the level of contamination can be controlled. In this present study, acetylcholinesterase (AChE) from the brain of <jats:italic>Monopterus albus</jats:italic> was elucidated to determine the potential alternative source of biosensor kit, which is sensitive towards various insecticides, especially carbamates. AChE from <jats:italic>M. albus</jats:italic> brain was extracted and purified through ammonium sulfate precipitation followed by procainamide-based affinity chromatography. Carbamate insecticides were used, such as bendiocarb, carbaryl, carbofuran, methomyl, and propoxur, to test their ability to inhibit AChE activity. Bendiocarb and methomyl show the capability to inhibit almost half of the enzyme activity at 51.05 and 51.20 %, respectively, while carbaryl, propoxur, and carbofuran inhibit 43.03, 42.80, and 15.06 %, respectively. Bendiocarb and methomyl were selected, and <jats:italic>M. albus</jats:italic> AChE was separately exposed with different concentrations of those carbamates and half maximal inhibitory concentration; IC50 was determined at 0.874 and 1.639 ppm, respectively. A field trial was conducted by testing the enzyme with various vegetable samples. All samples show no significant effect on AChE activity, meaning there was no existence of insecticides in each sample (p > 0.05). This study could be used as an alternative source for developing biosensor kits for the environmental monitoring program. IOP Publishing 2022 Article PeerReviewed Khalidi, S. A. M. and Sabullah,, M. K. and Gansau, J. A. and Faik, A. A. M. and Sani, S. A. and Shukor, M. Y. (2022) Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination. Journal of Physics: Conference Series, 2314. art. no. 12021. pp. 1-7. ISSN 1742-6588; ESSN: 1742-6596 https://iopscience.iop.org/article/10.1088/1742-6596/2314/1/012021/meta 10.1088/1742-6596/2314/1/012021 |
| spellingShingle | Khalidi, S. A. M. Sabullah,, M. K. Gansau, J. A. Faik, A. A. M. Sani, S. A. Shukor, M. Y. Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination |
| title | Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination |
| title_full | Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination |
| title_fullStr | Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination |
| title_full_unstemmed | Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination |
| title_short | Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination |
| title_sort | enzyme inhibition based biosensors using acetylcholinesterase from monopterus albus for detection of carbamates contamination |
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