Epidermal mucus of Anabas testudineus as a promising source of antibacterial and anticancer agents
Anabas testudineus is a sturdy freshwater fish that can live in a polluted environment due to the epidermal mucus (EM) that protects the fish from pathogens or germs. This study explored the functional properties of the EM as a potential antimicrobial and anticancer agent. Inactive Pseudomonas aerug...
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| Format: | Article |
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Universiti Kebangsaan Malaysia
2022
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| _version_ | 1825938518734536704 |
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| author | Najm, Ahmed Abdulkareem Eziwar Dyari, Herryawan Ryadi Othman, Babul Airianah Syed Alwi, Sharifah Sakinah Azfaralarriff, Ahmad Shahid, Muhammad Sanusi, Siti Aisyah Law, Douglas Shazrul Fazry |
| author_facet | Najm, Ahmed Abdulkareem Eziwar Dyari, Herryawan Ryadi Othman, Babul Airianah Syed Alwi, Sharifah Sakinah Azfaralarriff, Ahmad Shahid, Muhammad Sanusi, Siti Aisyah Law, Douglas Shazrul Fazry |
| author_sort | Najm, Ahmed Abdulkareem |
| collection | UPM |
| description | Anabas testudineus is a sturdy freshwater fish that can live in a polluted environment due to the epidermal mucus (EM) that protects the fish from pathogens or germs. This study explored the functional properties of the EM as a potential antimicrobial and anticancer agent. Inactive Pseudomonas aeruginosa was introduced into fish tanks to stimulate the production of EM. This stimulus significantly increased EM production by more than 100% after 10 days of stimulation, indicating that EM production was influenced by environmental biotic stress. In vitro antibacterial activity tests showed that EM has significant antibacterial activity against Escherichia coli (12 ± 0.23 mm) and P. aeruginosa (10 ± 0.13 mm) at the tested concentration of 1000 μg/mL. Further characterisation against cells showed that EM has a cytotoxic effect against human breast cancer (MCF7) and human melanoma (A375.S2) producing an IC50 value of 4.97 ± 0.25 and 6.27 ± 0.17 mg/mL, respectively. In contrast, no cytotoxicity against normal fibroblast skin cells (HS27) was observed. In addition, apoptosis analysis showed that EM could cause DNA fragmentation of cancer cells, while no effect on normal cells was observed. These findings indicated that EM from A. testudineus could be further studied and explored as an anticancer agent. |
| first_indexed | 2024-03-06T11:15:01Z |
| format | Article |
| id | upm.eprints-101292 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:15:01Z |
| publishDate | 2022 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | upm.eprints-1012922023-12-15T23:27:23Z http://psasir.upm.edu.my/id/eprint/101292/ Epidermal mucus of Anabas testudineus as a promising source of antibacterial and anticancer agents Najm, Ahmed Abdulkareem Eziwar Dyari, Herryawan Ryadi Othman, Babul Airianah Syed Alwi, Sharifah Sakinah Azfaralarriff, Ahmad Shahid, Muhammad Sanusi, Siti Aisyah Law, Douglas Shazrul Fazry Anabas testudineus is a sturdy freshwater fish that can live in a polluted environment due to the epidermal mucus (EM) that protects the fish from pathogens or germs. This study explored the functional properties of the EM as a potential antimicrobial and anticancer agent. Inactive Pseudomonas aeruginosa was introduced into fish tanks to stimulate the production of EM. This stimulus significantly increased EM production by more than 100% after 10 days of stimulation, indicating that EM production was influenced by environmental biotic stress. In vitro antibacterial activity tests showed that EM has significant antibacterial activity against Escherichia coli (12 ± 0.23 mm) and P. aeruginosa (10 ± 0.13 mm) at the tested concentration of 1000 μg/mL. Further characterisation against cells showed that EM has a cytotoxic effect against human breast cancer (MCF7) and human melanoma (A375.S2) producing an IC50 value of 4.97 ± 0.25 and 6.27 ± 0.17 mg/mL, respectively. In contrast, no cytotoxicity against normal fibroblast skin cells (HS27) was observed. In addition, apoptosis analysis showed that EM could cause DNA fragmentation of cancer cells, while no effect on normal cells was observed. These findings indicated that EM from A. testudineus could be further studied and explored as an anticancer agent. Universiti Kebangsaan Malaysia 2022 Article PeerReviewed Najm, Ahmed Abdulkareem and Eziwar Dyari, Herryawan Ryadi and Othman, Babul Airianah and Syed Alwi, Sharifah Sakinah and Azfaralarriff, Ahmad and Shahid, Muhammad and Sanusi, Siti Aisyah and Law, Douglas and Shazrul Fazry (2022) Epidermal mucus of Anabas testudineus as a promising source of antibacterial and anticancer agents. Penerbit Universiti Kebangsaan Malaysia, 51 (5). 1363 - 1372. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil5_2022/Jilid51Bil5_2022ms1363-1372.html 10.17576/jsm-2022-5105-08 |
| spellingShingle | Najm, Ahmed Abdulkareem Eziwar Dyari, Herryawan Ryadi Othman, Babul Airianah Syed Alwi, Sharifah Sakinah Azfaralarriff, Ahmad Shahid, Muhammad Sanusi, Siti Aisyah Law, Douglas Shazrul Fazry Epidermal mucus of Anabas testudineus as a promising source of antibacterial and anticancer agents |
| title | Epidermal mucus of Anabas testudineus as a promising source of antibacterial and anticancer agents |
| title_full | Epidermal mucus of Anabas testudineus as a promising source of antibacterial and anticancer agents |
| title_fullStr | Epidermal mucus of Anabas testudineus as a promising source of antibacterial and anticancer agents |
| title_full_unstemmed | Epidermal mucus of Anabas testudineus as a promising source of antibacterial and anticancer agents |
| title_short | Epidermal mucus of Anabas testudineus as a promising source of antibacterial and anticancer agents |
| title_sort | epidermal mucus of anabas testudineus as a promising source of antibacterial and anticancer agents |
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