Feather-degrading Bacillus cereus HD1: genomic analysis and its optimization for keratinase production and father degradation
Keratinase is an important enzyme that is used to degrade feather wastes produced by poultry industries and slaughterhouses that accumulate rapidly over time. The search for keratinase-producing microorganisms is important to potentially substitute physicochemical treatments of feather waste. In thi...
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| Format: | Article |
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Springer
2022
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| author | Radin Yahaya, Radin Shafierul Phang, Lai Yee M. Normi, Yahaya Abdullah, Janna Ong Ahmad, Siti Aqlima Sabri, Suriana |
| author_facet | Radin Yahaya, Radin Shafierul Phang, Lai Yee M. Normi, Yahaya Abdullah, Janna Ong Ahmad, Siti Aqlima Sabri, Suriana |
| author_sort | Radin Yahaya, Radin Shafierul |
| collection | UPM |
| description | Keratinase is an important enzyme that is used to degrade feather wastes produced by poultry industries and slaughterhouses that accumulate rapidly over time. The search for keratinase-producing microorganisms is important to potentially substitute physicochemical treatments of feather waste. In this study, the genome of Bacillus cereus HD1 and its keratinolytic prowess was investigated. The whole-genome shotgun size is 5,668,864 bp consisting of 6083 genes, 69 tRNAs, and 10 rRNAs. The genomic analyses revealed 15 potential keratinase genes and other enzymes that might assist keratin degradation, such as disulfide reductase and cysteine dioxygenase. The optimal conditions for feather degradation and keratinase production by B. cereus HD1 such as incubation time, pH, temperature, yeast extract, and glycerol concentrations were determined to be 5 days, pH 8, 37 °C, 0.05% (w/v), and 0.1% (v/v), respectively. Under optimized conditions, B. cereus HD1 exhibited feather degradation of 65%, with bacterial growth and maximum keratinase activity of 1.3 × 1011 CFU/mL and 41 U/mL, respectively, after 5 days of incubation in a feather basal medium. The findings obtained from this study may facilitate further research into utilizing B. cereus HD1 as a prominent keratinolytic enzymes production host and warrant potential biotechnological applications. |
| first_indexed | 2024-03-06T11:15:25Z |
| format | Article |
| id | upm.eprints-101507 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:15:25Z |
| publishDate | 2022 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-1015072023-08-22T03:39:08Z http://psasir.upm.edu.my/id/eprint/101507/ Feather-degrading Bacillus cereus HD1: genomic analysis and its optimization for keratinase production and father degradation Radin Yahaya, Radin Shafierul Phang, Lai Yee M. Normi, Yahaya Abdullah, Janna Ong Ahmad, Siti Aqlima Sabri, Suriana Keratinase is an important enzyme that is used to degrade feather wastes produced by poultry industries and slaughterhouses that accumulate rapidly over time. The search for keratinase-producing microorganisms is important to potentially substitute physicochemical treatments of feather waste. In this study, the genome of Bacillus cereus HD1 and its keratinolytic prowess was investigated. The whole-genome shotgun size is 5,668,864 bp consisting of 6083 genes, 69 tRNAs, and 10 rRNAs. The genomic analyses revealed 15 potential keratinase genes and other enzymes that might assist keratin degradation, such as disulfide reductase and cysteine dioxygenase. The optimal conditions for feather degradation and keratinase production by B. cereus HD1 such as incubation time, pH, temperature, yeast extract, and glycerol concentrations were determined to be 5 days, pH 8, 37 °C, 0.05% (w/v), and 0.1% (v/v), respectively. Under optimized conditions, B. cereus HD1 exhibited feather degradation of 65%, with bacterial growth and maximum keratinase activity of 1.3 × 1011 CFU/mL and 41 U/mL, respectively, after 5 days of incubation in a feather basal medium. The findings obtained from this study may facilitate further research into utilizing B. cereus HD1 as a prominent keratinolytic enzymes production host and warrant potential biotechnological applications. Springer 2022-04-23 Article PeerReviewed Radin Yahaya, Radin Shafierul and Phang, Lai Yee and M. Normi, Yahaya and Abdullah, Janna Ong and Ahmad, Siti Aqlima and Sabri, Suriana (2022) Feather-degrading Bacillus cereus HD1: genomic analysis and its optimization for keratinase production and father degradation. Current Microbiology, 79. art. no. 166. 166 - 181. ISSN 0343-8651; ESSN: 1432-0991 https://link.springer.com/article/10.1007/s00284-022-02861-1 10.1007/s00284-022-02861-1 |
| spellingShingle | Radin Yahaya, Radin Shafierul Phang, Lai Yee M. Normi, Yahaya Abdullah, Janna Ong Ahmad, Siti Aqlima Sabri, Suriana Feather-degrading Bacillus cereus HD1: genomic analysis and its optimization for keratinase production and father degradation |
| title | Feather-degrading Bacillus cereus HD1: genomic analysis and its optimization for keratinase production and father degradation |
| title_full | Feather-degrading Bacillus cereus HD1: genomic analysis and its optimization for keratinase production and father degradation |
| title_fullStr | Feather-degrading Bacillus cereus HD1: genomic analysis and its optimization for keratinase production and father degradation |
| title_full_unstemmed | Feather-degrading Bacillus cereus HD1: genomic analysis and its optimization for keratinase production and father degradation |
| title_short | Feather-degrading Bacillus cereus HD1: genomic analysis and its optimization for keratinase production and father degradation |
| title_sort | feather degrading bacillus cereus hd1 genomic analysis and its optimization for keratinase production and father degradation |
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