Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia

Astroviruses have been associated with enteric and extra-intestinal disorders in many animal species, including chickens. Here, we describe the detection and characterisation of chicken astrovirus (CAstV) in broilers and its seroprevalence in broiler breeder flocks. Based on PCR protocol, viral conf...

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Main Authors: Abdullahi Raji, Abdullahi, Ideris, Aini, Bejo, Mohd Hair, Omar, Abdul Rahman
Format: Article
Language:English
Published: Universiti Putra Malaysia 2022
Online Access:http://psasir.upm.edu.my/id/eprint/102226/1/43%20JST-3195-2021.pdf
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author Abdullahi Raji, Abdullahi
Ideris, Aini
Bejo, Mohd Hair
Omar, Abdul Rahman
author_facet Abdullahi Raji, Abdullahi
Ideris, Aini
Bejo, Mohd Hair
Omar, Abdul Rahman
author_sort Abdullahi Raji, Abdullahi
collection UPM
description Astroviruses have been associated with enteric and extra-intestinal disorders in many animal species, including chickens. Here, we describe the detection and characterisation of chicken astrovirus (CAstV) in broilers and its seroprevalence in broiler breeder flocks. Based on PCR protocol, viral confirmation was carried out on clinical tissue samples from broiler chickens suffering from uneven growth and poor performance. The tissues were molecularly detected for CAstV with differential diagnostic testing against the Newcastle disease virus, infectious bronchitis virus, avian nephritis virus, avian rotavirus, fowl adenovirus and avian reovirus. Polymerase gene-based phylogenetic analyses of the twenty samples detected positive for CAstV indicate they belong to Group I and are related to strains from the US, UK, India and Poland. From these 20 samples, CastV could be isolated from 3 samples upon inoculation in 5-day-old specific-pathogen-free (SPF) embryonated chicken eggs (ECE); virus-infected embryos showed dwarfing, haemorrhages, oedema and gelatinous lesions at harvest. The enzyme-linked immunosorbent assay (ELISA) results revealed a high prevalence of antibodies against CAstV amongst the broiler breeder flocks tested. It is the first study that describes the detection and prevalence of CAstV in broiler chickens and broiler breeder flocks in Malaysia.
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spelling upm.eprints-1022262023-08-11T08:19:05Z http://psasir.upm.edu.my/id/eprint/102226/ Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia Abdullahi Raji, Abdullahi Ideris, Aini Bejo, Mohd Hair Omar, Abdul Rahman Astroviruses have been associated with enteric and extra-intestinal disorders in many animal species, including chickens. Here, we describe the detection and characterisation of chicken astrovirus (CAstV) in broilers and its seroprevalence in broiler breeder flocks. Based on PCR protocol, viral confirmation was carried out on clinical tissue samples from broiler chickens suffering from uneven growth and poor performance. The tissues were molecularly detected for CAstV with differential diagnostic testing against the Newcastle disease virus, infectious bronchitis virus, avian nephritis virus, avian rotavirus, fowl adenovirus and avian reovirus. Polymerase gene-based phylogenetic analyses of the twenty samples detected positive for CAstV indicate they belong to Group I and are related to strains from the US, UK, India and Poland. From these 20 samples, CastV could be isolated from 3 samples upon inoculation in 5-day-old specific-pathogen-free (SPF) embryonated chicken eggs (ECE); virus-infected embryos showed dwarfing, haemorrhages, oedema and gelatinous lesions at harvest. The enzyme-linked immunosorbent assay (ELISA) results revealed a high prevalence of antibodies against CAstV amongst the broiler breeder flocks tested. It is the first study that describes the detection and prevalence of CAstV in broiler chickens and broiler breeder flocks in Malaysia. Universiti Putra Malaysia 2022-04-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/102226/1/43%20JST-3195-2021.pdf Abdullahi Raji, Abdullahi and Ideris, Aini and Bejo, Mohd Hair and Omar, Abdul Rahman (2022) Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia. Pertanika Journal of Science and Technology, 30 (2). 1641 - 1655. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-3195-2021 10.47836/pjst.30.2.43
spellingShingle Abdullahi Raji, Abdullahi
Ideris, Aini
Bejo, Mohd Hair
Omar, Abdul Rahman
Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia
title Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia
title_full Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia
title_fullStr Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia
title_full_unstemmed Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia
title_short Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia
title_sort molecular detection characterisation and serological survey of chicken astrovirus from broiler flocks in malaysia
url http://psasir.upm.edu.my/id/eprint/102226/1/43%20JST-3195-2021.pdf
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