Nuclear magnetic resonance-based metabolomics study of serum and pectoralis major for different commercial chicken breeds
This study aimed to characterize the metabolic composition of four types of commercially available chicken breeds [village chicken, colored broiler (Hubbard), broiler (Cobb), and spent layers (Dekalb)] by 1H NMR coupling and discriminate them using multivariate analysis. Five chickens were collected...
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| Format: | Article |
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John Wiley & Sons
2023
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| _version_ | 1796984207064432640 |
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| author | Tan, Chengkeng Selamat, Jinap Jambari, Nuzul Noorahya Sukor, Rashidah Murugesu, Suganya Muhamad, Azira Khatib, Alfi |
| author_facet | Tan, Chengkeng Selamat, Jinap Jambari, Nuzul Noorahya Sukor, Rashidah Murugesu, Suganya Muhamad, Azira Khatib, Alfi |
| author_sort | Tan, Chengkeng |
| collection | UPM |
| description | This study aimed to characterize the metabolic composition of four types of commercially available chicken breeds [village chicken, colored broiler (Hubbard), broiler (Cobb), and spent layers (Dekalb)] by 1H NMR coupling and discriminate them using multivariate analysis. Five chickens were collected for each chicken breed based on the marketing age from the respective commercial farms. The orthogonal partial least squares discriminant analysis (OPLS-DA) results showed an obvious separation of local village chickens from the other breeds based on the metabolites present in their serum and meat (pectoralis major). The cumulative values of Q2, R2X, and R2Y of the OPLS-DA model for chicken serum were 0.722, 0.877, and 0.841. For the pectoralis major muscle, the cumulative values of Q2, R2X, and R2Y of the OPLS-DA model were reported as 0.684, 0.781, and 0.786, respectively. The quality of both OPLS-DA models was accepted by the cumulative values of Q2 ≥ 0.5 and R2 ≥ 0.65. The 1H NMR result with multivariate analysis has successfully distinguished local village chicken from the other three commercial chicken breeds based on serum and pectoralis major muscle. Nonetheless, colored broiler (Hubbard) was not distinguished from broiler (Cobb) and spent layers (Dekalb) in serum and pectoralis major, respectively. The OPLS-DA assessment in this study identified 19 and 15 potential metabolites for discriminating different chicken breeds in serum and pectoralis major muscle, respectively. Some of the prominent metabolites identified include amino acids (betaine, glycine, glutamine, guanidoacetate, phenylalanine, and valine), nucleotides (IMP and NAD+), organic acids (lactate, malate, and succinate), peptide (anserine), and sugar alcohol (myo-inositol). |
| first_indexed | 2024-03-06T11:17:11Z |
| format | Article |
| id | upm.eprints-102334 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:17:11Z |
| publishDate | 2023 |
| publisher | John Wiley & Sons |
| record_format | dspace |
| spelling | upm.eprints-1023342023-05-22T02:00:41Z http://psasir.upm.edu.my/id/eprint/102334/ Nuclear magnetic resonance-based metabolomics study of serum and pectoralis major for different commercial chicken breeds Tan, Chengkeng Selamat, Jinap Jambari, Nuzul Noorahya Sukor, Rashidah Murugesu, Suganya Muhamad, Azira Khatib, Alfi This study aimed to characterize the metabolic composition of four types of commercially available chicken breeds [village chicken, colored broiler (Hubbard), broiler (Cobb), and spent layers (Dekalb)] by 1H NMR coupling and discriminate them using multivariate analysis. Five chickens were collected for each chicken breed based on the marketing age from the respective commercial farms. The orthogonal partial least squares discriminant analysis (OPLS-DA) results showed an obvious separation of local village chickens from the other breeds based on the metabolites present in their serum and meat (pectoralis major). The cumulative values of Q2, R2X, and R2Y of the OPLS-DA model for chicken serum were 0.722, 0.877, and 0.841. For the pectoralis major muscle, the cumulative values of Q2, R2X, and R2Y of the OPLS-DA model were reported as 0.684, 0.781, and 0.786, respectively. The quality of both OPLS-DA models was accepted by the cumulative values of Q2 ≥ 0.5 and R2 ≥ 0.65. The 1H NMR result with multivariate analysis has successfully distinguished local village chicken from the other three commercial chicken breeds based on serum and pectoralis major muscle. Nonetheless, colored broiler (Hubbard) was not distinguished from broiler (Cobb) and spent layers (Dekalb) in serum and pectoralis major, respectively. The OPLS-DA assessment in this study identified 19 and 15 potential metabolites for discriminating different chicken breeds in serum and pectoralis major muscle, respectively. Some of the prominent metabolites identified include amino acids (betaine, glycine, glutamine, guanidoacetate, phenylalanine, and valine), nucleotides (IMP and NAD+), organic acids (lactate, malate, and succinate), peptide (anserine), and sugar alcohol (myo-inositol). John Wiley & Sons 2023-04-07 Article PeerReviewed Tan, Chengkeng and Selamat, Jinap and Jambari, Nuzul Noorahya and Sukor, Rashidah and Murugesu, Suganya and Muhamad, Azira and Khatib, Alfi (2023) Nuclear magnetic resonance-based metabolomics study of serum and pectoralis major for different commercial chicken breeds. Food Science & Nutrition, 11 (5). pp. 2106-2117. ISSN 2048-7177 https://onlinelibrary.wiley.com/doi/10.1002/fsn3.2968 10.1002/fsn3.2968 |
| spellingShingle | Tan, Chengkeng Selamat, Jinap Jambari, Nuzul Noorahya Sukor, Rashidah Murugesu, Suganya Muhamad, Azira Khatib, Alfi Nuclear magnetic resonance-based metabolomics study of serum and pectoralis major for different commercial chicken breeds |
| title | Nuclear magnetic resonance-based metabolomics study of serum and pectoralis major for different commercial chicken breeds |
| title_full | Nuclear magnetic resonance-based metabolomics study of serum and pectoralis major for different commercial chicken breeds |
| title_fullStr | Nuclear magnetic resonance-based metabolomics study of serum and pectoralis major for different commercial chicken breeds |
| title_full_unstemmed | Nuclear magnetic resonance-based metabolomics study of serum and pectoralis major for different commercial chicken breeds |
| title_short | Nuclear magnetic resonance-based metabolomics study of serum and pectoralis major for different commercial chicken breeds |
| title_sort | nuclear magnetic resonance based metabolomics study of serum and pectoralis major for different commercial chicken breeds |
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