Ultrastructural and morphological effects in T-lymphoblastic leukemia CEM-SS cells following treatment with nordamnacanthal and damnacanthal from roots of Morinda elliptica
Background: Morinda elliptica (family Rubiaceae), locally known as ‘mengkudu kecil’, has been used by the Malays for medicinal purposes. Anthraquinones isolated from the roots of Morinda elliptica, namely nordamnacanthal and damnacanthal, have been widely reported to exhibit anticancer and antioxida...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
MDPI AG
2022
|
| _version_ | 1796984237208895488 |
|---|---|
| author | Latifah, Saiful Yazan Gopalsamy, Banulata Abdul Rahim, Raha Ali, Abdul Manaf Haji Lajis, Nordin |
| author_facet | Latifah, Saiful Yazan Gopalsamy, Banulata Abdul Rahim, Raha Ali, Abdul Manaf Haji Lajis, Nordin |
| author_sort | Latifah, Saiful Yazan |
| collection | UPM |
| description | Background: Morinda elliptica (family Rubiaceae), locally known as ‘mengkudu kecil’, has been used by the Malays for medicinal purposes. Anthraquinones isolated from the roots of Morinda elliptica, namely nordamnacanthal and damnacanthal, have been widely reported to exhibit anticancer and antioxidant properties in various cancer models in vitro and in vivo. Aim: This study analyzed the morphological and ultrastructural effects of damnacanthal and nordamnacanthal on T-lymphoblastic leukemia CEM-SS cells. Method: Light microscopy, Giemsa staining, Wright’s staining, scanning electron microscopy, and transmission electron microscopy were carried out to determine apoptosis, necrosis, and ultrastructural changes that occurred within the cells. Results: The outcomes showed that these compounds induced cell death by apoptosis and necrosis, specifically at higher doses of 10 and 30 μg/mL. Condensation and fragmentation of the nuclear chromatin, which further separated into small, membrane-bound vesicles known as apoptotic bodies, were observed in the nuclei and cytoplasm. The plasma membranes and cytoskeletons also showed marked morphological changes upon treatment with damnacanthal and nordamnacanthal, indicating apoptosis. Conclusion: Therefore, we report that damnacanthal and nordamnacanthal exhibit anticancer properties by inducing apoptosis and necrosis in CEM-SS cells, and they have potential as a drug for the treatment of T-lymphoblastic leukemia. |
| first_indexed | 2024-04-09T03:50:37Z |
| format | Article |
| id | upm.eprints-102525 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-04-09T03:50:37Z |
| publishDate | 2022 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | upm.eprints-1025252024-03-14T04:54:12Z http://psasir.upm.edu.my/id/eprint/102525/ Ultrastructural and morphological effects in T-lymphoblastic leukemia CEM-SS cells following treatment with nordamnacanthal and damnacanthal from roots of Morinda elliptica Latifah, Saiful Yazan Gopalsamy, Banulata Abdul Rahim, Raha Ali, Abdul Manaf Haji Lajis, Nordin Background: Morinda elliptica (family Rubiaceae), locally known as ‘mengkudu kecil’, has been used by the Malays for medicinal purposes. Anthraquinones isolated from the roots of Morinda elliptica, namely nordamnacanthal and damnacanthal, have been widely reported to exhibit anticancer and antioxidant properties in various cancer models in vitro and in vivo. Aim: This study analyzed the morphological and ultrastructural effects of damnacanthal and nordamnacanthal on T-lymphoblastic leukemia CEM-SS cells. Method: Light microscopy, Giemsa staining, Wright’s staining, scanning electron microscopy, and transmission electron microscopy were carried out to determine apoptosis, necrosis, and ultrastructural changes that occurred within the cells. Results: The outcomes showed that these compounds induced cell death by apoptosis and necrosis, specifically at higher doses of 10 and 30 μg/mL. Condensation and fragmentation of the nuclear chromatin, which further separated into small, membrane-bound vesicles known as apoptotic bodies, were observed in the nuclei and cytoplasm. The plasma membranes and cytoskeletons also showed marked morphological changes upon treatment with damnacanthal and nordamnacanthal, indicating apoptosis. Conclusion: Therefore, we report that damnacanthal and nordamnacanthal exhibit anticancer properties by inducing apoptosis and necrosis in CEM-SS cells, and they have potential as a drug for the treatment of T-lymphoblastic leukemia. MDPI AG 2022-06 Article PeerReviewed Latifah, Saiful Yazan and Gopalsamy, Banulata and Abdul Rahim, Raha and Ali, Abdul Manaf and Haji Lajis, Nordin (2022) Ultrastructural and morphological effects in T-lymphoblastic leukemia CEM-SS cells following treatment with nordamnacanthal and damnacanthal from roots of Morinda elliptica. Molecules: a journal of synthetic organic and natural product chemistry, 27 (13). art. no. 4136. pp. 1-21. ISSN 1420-3049 https://www.mdpi.com/1420-3049/27/13/4136 10.3390/molecules27134136 |
| spellingShingle | Latifah, Saiful Yazan Gopalsamy, Banulata Abdul Rahim, Raha Ali, Abdul Manaf Haji Lajis, Nordin Ultrastructural and morphological effects in T-lymphoblastic leukemia CEM-SS cells following treatment with nordamnacanthal and damnacanthal from roots of Morinda elliptica |
| title | Ultrastructural and morphological effects in T-lymphoblastic leukemia CEM-SS cells following treatment with nordamnacanthal and damnacanthal from roots of Morinda elliptica |
| title_full | Ultrastructural and morphological effects in T-lymphoblastic leukemia CEM-SS cells following treatment with nordamnacanthal and damnacanthal from roots of Morinda elliptica |
| title_fullStr | Ultrastructural and morphological effects in T-lymphoblastic leukemia CEM-SS cells following treatment with nordamnacanthal and damnacanthal from roots of Morinda elliptica |
| title_full_unstemmed | Ultrastructural and morphological effects in T-lymphoblastic leukemia CEM-SS cells following treatment with nordamnacanthal and damnacanthal from roots of Morinda elliptica |
| title_short | Ultrastructural and morphological effects in T-lymphoblastic leukemia CEM-SS cells following treatment with nordamnacanthal and damnacanthal from roots of Morinda elliptica |
| title_sort | ultrastructural and morphological effects in t lymphoblastic leukemia cem ss cells following treatment with nordamnacanthal and damnacanthal from roots of morinda elliptica |
| work_keys_str_mv | AT latifahsaifulyazan ultrastructuralandmorphologicaleffectsintlymphoblasticleukemiacemsscellsfollowingtreatmentwithnordamnacanthalanddamnacanthalfromrootsofmorindaelliptica AT gopalsamybanulata ultrastructuralandmorphologicaleffectsintlymphoblasticleukemiacemsscellsfollowingtreatmentwithnordamnacanthalanddamnacanthalfromrootsofmorindaelliptica AT abdulrahimraha ultrastructuralandmorphologicaleffectsintlymphoblasticleukemiacemsscellsfollowingtreatmentwithnordamnacanthalanddamnacanthalfromrootsofmorindaelliptica AT aliabdulmanaf ultrastructuralandmorphologicaleffectsintlymphoblasticleukemiacemsscellsfollowingtreatmentwithnordamnacanthalanddamnacanthalfromrootsofmorindaelliptica AT hajilajisnordin ultrastructuralandmorphologicaleffectsintlymphoblasticleukemiacemsscellsfollowingtreatmentwithnordamnacanthalanddamnacanthalfromrootsofmorindaelliptica |