Colletotrichum Disease of Cocoa (Theobroma Cacao L.) and its Control

Isolation of disease pathogen from infected cocoa leaves and pods associated with leaf spot and pod rot disease yielded Colletotrichum gloeosporioides. No distinct differences in cultural and morphological characteristics were noted from the various isolates. Further investigation by polyacryla...

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Main Author: Yee, Ming Fatt
Format: Thesis
Language:English
English
Published: 1991
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/10302/1/FP_1991_11_A.pdf
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author Yee, Ming Fatt
author_facet Yee, Ming Fatt
author_sort Yee, Ming Fatt
collection UPM
description Isolation of disease pathogen from infected cocoa leaves and pods associated with leaf spot and pod rot disease yielded Colletotrichum gloeosporioides. No distinct differences in cultural and morphological characteristics were noted from the various isolates. Further investigation by polyacrylamide vertical gel electrophoresis indicated that protein, esterase and peroxidase patterns were unable to differentiate strain gloeosporioides. variation within C. The fungus was found to grow and sporulate well at 30°C. Cocoa Leaf Extract Agar (CLEA) was the best medium for mycelial growth while Potato Dextrose Agar (PDA) appeared to favour sporulation. Better mycelial growth was also achieved on substrates of neutral condition while extreme alkaline condition induces sporulation. Both cocoa leaves and injured pods were liable to infection by C. gloeosporioides. Three-week old cocoa seedlings and cherelles were noted as the most susceptible stage. The development of Colletotrichum leaf spot disease invariably involved three phases of activity viz., prepenetration phase which involved spore germination and formation of appressoria, penetration phase involving intercellular and intracellular hyphal penetration and postpenetration phase resulting in disintegration of cells and the formation of acervuli.
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spelling upm.eprints-103022011-03-22T00:48:38Z http://psasir.upm.edu.my/id/eprint/10302/ Colletotrichum Disease of Cocoa (Theobroma Cacao L.) and its Control Yee, Ming Fatt Isolation of disease pathogen from infected cocoa leaves and pods associated with leaf spot and pod rot disease yielded Colletotrichum gloeosporioides. No distinct differences in cultural and morphological characteristics were noted from the various isolates. Further investigation by polyacrylamide vertical gel electrophoresis indicated that protein, esterase and peroxidase patterns were unable to differentiate strain gloeosporioides. variation within C. The fungus was found to grow and sporulate well at 30°C. Cocoa Leaf Extract Agar (CLEA) was the best medium for mycelial growth while Potato Dextrose Agar (PDA) appeared to favour sporulation. Better mycelial growth was also achieved on substrates of neutral condition while extreme alkaline condition induces sporulation. Both cocoa leaves and injured pods were liable to infection by C. gloeosporioides. Three-week old cocoa seedlings and cherelles were noted as the most susceptible stage. The development of Colletotrichum leaf spot disease invariably involved three phases of activity viz., prepenetration phase which involved spore germination and formation of appressoria, penetration phase involving intercellular and intracellular hyphal penetration and postpenetration phase resulting in disintegration of cells and the formation of acervuli. 1991 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/10302/1/FP_1991_11_A.pdf Yee, Ming Fatt (1991) Colletotrichum Disease of Cocoa (Theobroma Cacao L.) and its Control. Masters thesis, Universiti Pertanian Malaysia. Cocoa - Diseases - Case studies English
spellingShingle Cocoa - Diseases - Case studies
Yee, Ming Fatt
Colletotrichum Disease of Cocoa (Theobroma Cacao L.) and its Control
title Colletotrichum Disease of Cocoa (Theobroma Cacao L.) and its Control
title_full Colletotrichum Disease of Cocoa (Theobroma Cacao L.) and its Control
title_fullStr Colletotrichum Disease of Cocoa (Theobroma Cacao L.) and its Control
title_full_unstemmed Colletotrichum Disease of Cocoa (Theobroma Cacao L.) and its Control
title_short Colletotrichum Disease of Cocoa (Theobroma Cacao L.) and its Control
title_sort colletotrichum disease of cocoa theobroma cacao l and its control
topic Cocoa - Diseases - Case studies
url http://psasir.upm.edu.my/id/eprint/10302/1/FP_1991_11_A.pdf
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