Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease
: This study was aimed to evaluate the potential of several carriers to formulate the phages and retain their activity under various pH and temperature conditions. Methodology and results: The skim milk, rice flour, corn flour and CalnuXan (calcium and magnesium) as carriers to formulate the isol...
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| Format: | Article |
| Published: |
Malaysian Society of Microbiology
2022
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| _version_ | 1825938800202743808 |
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| author | Liu, Jian Wang, Huiling Chia, Suet Lin Tan, Geok Hun |
| author_facet | Liu, Jian Wang, Huiling Chia, Suet Lin Tan, Geok Hun |
| author_sort | Liu, Jian |
| collection | UPM |
| description | : This study was aimed to evaluate the potential of several carriers to formulate the phages and retain their activity
under various pH and temperature conditions.
Methodology and results: The skim milk, rice flour, corn flour and CalnuXan (calcium and magnesium) as carriers to
formulate the isolated phage to maintain its activity under extreme pH and temperature conditions. Two phages
formulated with carriers retained their viability at pH 5, pH 7 and pH 9 compared to that of the unformulated phages.
Besides, the formulated phages also retained a high titre compared to the unformulated phages when they were
exposed to 37 °C and 45 °C. Based on the in vitro study of the formulation, it was applied in the glass house. The plant
height, leaf chlorophyll and disease scoring were recorded and analyzed. In the glass house, the rice plant treated with
formulated phages showed higher plant height and chlorophyll content than those treated with unformulated or untreated
phages. Nonetheless, both formulated and unformulated protected the rice plant, which showed lower disease severity
than the untreated group. Conclusion, significance and impact of study: Phage therapy has been used for treating plant diseases caused by pathogenic bacteria. Despite their effectiveness in killing the pathogen in vitro, the results were not reproducible in the field. Bacteriophages (phages) are sensitive to environmental factors and infection efficiency was dropped when exposed to harmful environments. However, this study successfully formulated two novels Xanthomonas phages, as biocontrol agents against bacterial leaf blight (BLB) disease in rice. |
| first_indexed | 2024-03-06T11:17:49Z |
| format | Article |
| id | upm.eprints-103141 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:17:49Z |
| publishDate | 2022 |
| publisher | Malaysian Society of Microbiology |
| record_format | dspace |
| spelling | upm.eprints-1031412023-12-05T04:03:33Z http://psasir.upm.edu.my/id/eprint/103141/ Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease Liu, Jian Wang, Huiling Chia, Suet Lin Tan, Geok Hun : This study was aimed to evaluate the potential of several carriers to formulate the phages and retain their activity under various pH and temperature conditions. Methodology and results: The skim milk, rice flour, corn flour and CalnuXan (calcium and magnesium) as carriers to formulate the isolated phage to maintain its activity under extreme pH and temperature conditions. Two phages formulated with carriers retained their viability at pH 5, pH 7 and pH 9 compared to that of the unformulated phages. Besides, the formulated phages also retained a high titre compared to the unformulated phages when they were exposed to 37 °C and 45 °C. Based on the in vitro study of the formulation, it was applied in the glass house. The plant height, leaf chlorophyll and disease scoring were recorded and analyzed. In the glass house, the rice plant treated with formulated phages showed higher plant height and chlorophyll content than those treated with unformulated or untreated phages. Nonetheless, both formulated and unformulated protected the rice plant, which showed lower disease severity than the untreated group. Conclusion, significance and impact of study: Phage therapy has been used for treating plant diseases caused by pathogenic bacteria. Despite their effectiveness in killing the pathogen in vitro, the results were not reproducible in the field. Bacteriophages (phages) are sensitive to environmental factors and infection efficiency was dropped when exposed to harmful environments. However, this study successfully formulated two novels Xanthomonas phages, as biocontrol agents against bacterial leaf blight (BLB) disease in rice. Malaysian Society of Microbiology 2022 Article PeerReviewed Liu, Jian and Wang, Huiling and Chia, Suet Lin and Tan, Geok Hun (2022) Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease. Malaysian Journal of Microbiology, 18 (5). 490 - 504. ISSN 1823-8262; ESSN: 2231-7538 https://mjm.usm.my/ 10.21161/mjm.221435 |
| spellingShingle | Liu, Jian Wang, Huiling Chia, Suet Lin Tan, Geok Hun Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease |
| title | Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease |
| title_full | Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease |
| title_fullStr | Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease |
| title_full_unstemmed | Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease |
| title_short | Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease |
| title_sort | screening and formulation of novel carriers for xanthomonas bacteriophage to control bacterial leaf blight disease |
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