Sinapine improves LPS-induced oxidative stress in hepatocytes by down-regulating MCJ protein expression
Oxidative stress is an important part of the development of NAFLD, and hepatic injury can be prevented by inhibiting oxidative stress. In this study, we investigated the potential role of sinapine in protecting the liver. LPS was selected to establish the oxidative stress model of THLE-2 cells, and...
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| Format: | Article |
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Elsevier
2022
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| _version_ | 1796984267739234304 |
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| author | Li, Youdong Xu, Yong-Jiang Tan, Chin Ping Liu, Yuanfa |
| author_facet | Li, Youdong Xu, Yong-Jiang Tan, Chin Ping Liu, Yuanfa |
| author_sort | Li, Youdong |
| collection | UPM |
| description | Oxidative stress is an important part of the development of NAFLD, and hepatic injury can be prevented by inhibiting oxidative stress. In this study, we investigated the potential role of sinapine in protecting the liver. LPS was selected to establish the oxidative stress model of THLE-2 cells, and the treatment concentrations of LPS (5 μg/mL) and sinapine (5 μM, 20 μM, and 80 μM) were determined by toxicity experiments. The MDA of the sinapine (80 μM) pretreatment group was 1.09 ± 0.13 nmol/mg prot which was reduced by 27.67 % compared with the LPS group. Furthermore, SOD and GSH-Px levels were significantly increased by 40.61 % and 49.60 %, respectively. And the ROS levels of 20 and 80 μM sinapine were reduced by 31.47 % and 40.31 %, respectively (p < 0.01) compared with the model group. The mitochondrial membrane potential had similar results. It was also found that sinapine can significantly down-regulate the level of MCJ protein (p < 0.01), which is related to oxidative stress. Our results indicate that sinapine can maintain liver health by down-regulating the expression of MCJ protein to inhibit oxidative stress, which provides a theoretical basis for the use of sinapine as an inhibitor of MCJ. |
| first_indexed | 2024-03-06T11:17:55Z |
| format | Article |
| id | upm.eprints-103207 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:17:55Z |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-1032072023-11-30T02:40:56Z http://psasir.upm.edu.my/id/eprint/103207/ Sinapine improves LPS-induced oxidative stress in hepatocytes by down-regulating MCJ protein expression Li, Youdong Xu, Yong-Jiang Tan, Chin Ping Liu, Yuanfa Oxidative stress is an important part of the development of NAFLD, and hepatic injury can be prevented by inhibiting oxidative stress. In this study, we investigated the potential role of sinapine in protecting the liver. LPS was selected to establish the oxidative stress model of THLE-2 cells, and the treatment concentrations of LPS (5 μg/mL) and sinapine (5 μM, 20 μM, and 80 μM) were determined by toxicity experiments. The MDA of the sinapine (80 μM) pretreatment group was 1.09 ± 0.13 nmol/mg prot which was reduced by 27.67 % compared with the LPS group. Furthermore, SOD and GSH-Px levels were significantly increased by 40.61 % and 49.60 %, respectively. And the ROS levels of 20 and 80 μM sinapine were reduced by 31.47 % and 40.31 %, respectively (p < 0.01) compared with the model group. The mitochondrial membrane potential had similar results. It was also found that sinapine can significantly down-regulate the level of MCJ protein (p < 0.01), which is related to oxidative stress. Our results indicate that sinapine can maintain liver health by down-regulating the expression of MCJ protein to inhibit oxidative stress, which provides a theoretical basis for the use of sinapine as an inhibitor of MCJ. Elsevier 2022 Article PeerReviewed Li, Youdong and Xu, Yong-Jiang and Tan, Chin Ping and Liu, Yuanfa (2022) Sinapine improves LPS-induced oxidative stress in hepatocytes by down-regulating MCJ protein expression. Life Sciences, 306. art. no. 120828. pp. 1-11. ISSN 0024-3205; ESSN: 1879-0631 https://www.sciencedirect.com/science/article/pii/S0024320522005288 10.1016/j.lfs.2022.120828 |
| spellingShingle | Li, Youdong Xu, Yong-Jiang Tan, Chin Ping Liu, Yuanfa Sinapine improves LPS-induced oxidative stress in hepatocytes by down-regulating MCJ protein expression |
| title | Sinapine improves LPS-induced oxidative stress in hepatocytes by down-regulating MCJ protein expression |
| title_full | Sinapine improves LPS-induced oxidative stress in hepatocytes by down-regulating MCJ protein expression |
| title_fullStr | Sinapine improves LPS-induced oxidative stress in hepatocytes by down-regulating MCJ protein expression |
| title_full_unstemmed | Sinapine improves LPS-induced oxidative stress in hepatocytes by down-regulating MCJ protein expression |
| title_short | Sinapine improves LPS-induced oxidative stress in hepatocytes by down-regulating MCJ protein expression |
| title_sort | sinapine improves lps induced oxidative stress in hepatocytes by down regulating mcj protein expression |
| work_keys_str_mv | AT liyoudong sinapineimproveslpsinducedoxidativestressinhepatocytesbydownregulatingmcjproteinexpression AT xuyongjiang sinapineimproveslpsinducedoxidativestressinhepatocytesbydownregulatingmcjproteinexpression AT tanchinping sinapineimproveslpsinducedoxidativestressinhepatocytesbydownregulatingmcjproteinexpression AT liuyuanfa sinapineimproveslpsinducedoxidativestressinhepatocytesbydownregulatingmcjproteinexpression |