Skin mucus proteome analysis reveals disease-resistant biomarker signatures in hybrid grouper Epinephelus fuscoguttatus a x Epinephelus lanceolatus a‚ against Vibrio alginolyticus
Fish skin mucus is the first line of defense that provides physical and chemical barriers against pathogens and toxins. The mucus is produced continuously and sloughed off regularly from the skin to defend against infections through the skin. However, the molecular properties of the mucus content th...
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| Format: | Article |
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MDPI
2022
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| _version_ | 1811137656141119488 |
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| author | Nurhikmah Christianus, Annie Wan Solahudin, Wan Mohd Syazwan Lau, Benjamin Yii Chung Ismail, Intan Safinar Fei, Low Chen |
| author_facet | Nurhikmah Christianus, Annie Wan Solahudin, Wan Mohd Syazwan Lau, Benjamin Yii Chung Ismail, Intan Safinar Fei, Low Chen |
| author_sort | Nurhikmah |
| collection | UPM |
| description | Fish skin mucus is the first line of defense that provides physical and chemical barriers against pathogens and toxins. The mucus is produced continuously and sloughed off regularly from the skin to defend against infections through the skin. However, the molecular properties of the mucus content that prevent pathogen invasion are yet to be fully understood. In this study, a proteomic approach using liquid chromatography–mass spectrometry (LCMS) was applied to explore the changes in the mucus protein content of resistant and susceptible groupers in response to Vibrio alginolyticus. The Vibrio-resistant groupers showed no observable clinical sign of infection after the immersion challenge, while the Vibrio-susceptible groupers presented either hemorrhagic- or non-hemorrhagic ulceration of the skin. A comparative proteome analysis on the mucus samples yielded 1488 identified proteins. The immune-related proteins, namely Cystatin B, Complement Component C6, Complement factor 1, Allograft inflammatory factor 1, Deleted in malignant brain tumors protein, MHC class 1 and Annexin A1, that were significantly abundant in the resistant group responded to V. alginolyticus infection. Interestingly, there was an expression of immune-related proteins that possibly could be the non-invasive biomarkers, namely 3-hydroxybutyrate dehydrogenase type 2 and L-rhamnose-binding lectin SML. |
| first_indexed | 2024-09-25T03:37:46Z |
| format | Article |
| id | upm.eprints-103213 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:37:46Z |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-1032132024-06-23T01:23:47Z http://psasir.upm.edu.my/id/eprint/103213/ Skin mucus proteome analysis reveals disease-resistant biomarker signatures in hybrid grouper Epinephelus fuscoguttatus a x Epinephelus lanceolatus a‚ against Vibrio alginolyticus Nurhikmah Christianus, Annie Wan Solahudin, Wan Mohd Syazwan Lau, Benjamin Yii Chung Ismail, Intan Safinar Fei, Low Chen Fish skin mucus is the first line of defense that provides physical and chemical barriers against pathogens and toxins. The mucus is produced continuously and sloughed off regularly from the skin to defend against infections through the skin. However, the molecular properties of the mucus content that prevent pathogen invasion are yet to be fully understood. In this study, a proteomic approach using liquid chromatography–mass spectrometry (LCMS) was applied to explore the changes in the mucus protein content of resistant and susceptible groupers in response to Vibrio alginolyticus. The Vibrio-resistant groupers showed no observable clinical sign of infection after the immersion challenge, while the Vibrio-susceptible groupers presented either hemorrhagic- or non-hemorrhagic ulceration of the skin. A comparative proteome analysis on the mucus samples yielded 1488 identified proteins. The immune-related proteins, namely Cystatin B, Complement Component C6, Complement factor 1, Allograft inflammatory factor 1, Deleted in malignant brain tumors protein, MHC class 1 and Annexin A1, that were significantly abundant in the resistant group responded to V. alginolyticus infection. Interestingly, there was an expression of immune-related proteins that possibly could be the non-invasive biomarkers, namely 3-hydroxybutyrate dehydrogenase type 2 and L-rhamnose-binding lectin SML. MDPI 2022 Article PeerReviewed Nurhikmah and Christianus, Annie and Wan Solahudin, Wan Mohd Syazwan and Lau, Benjamin Yii Chung and Ismail, Intan Safinar and Fei, Low Chen (2022) Skin mucus proteome analysis reveals disease-resistant biomarker signatures in hybrid grouper Epinephelus fuscoguttatus a x Epinephelus lanceolatus a‚ against Vibrio alginolyticus. Fishes, 7 (5). art. no. 278. pp. 1-28. ISSN 2410-3888 https://www.mdpi.com/2410-3888/7/5/278 10.3390/fishes7050278 |
| spellingShingle | Nurhikmah Christianus, Annie Wan Solahudin, Wan Mohd Syazwan Lau, Benjamin Yii Chung Ismail, Intan Safinar Fei, Low Chen Skin mucus proteome analysis reveals disease-resistant biomarker signatures in hybrid grouper Epinephelus fuscoguttatus a x Epinephelus lanceolatus a‚ against Vibrio alginolyticus |
| title | Skin mucus proteome analysis reveals disease-resistant biomarker signatures in hybrid grouper Epinephelus fuscoguttatus a x Epinephelus lanceolatus a‚ against Vibrio alginolyticus |
| title_full | Skin mucus proteome analysis reveals disease-resistant biomarker signatures in hybrid grouper Epinephelus fuscoguttatus a x Epinephelus lanceolatus a‚ against Vibrio alginolyticus |
| title_fullStr | Skin mucus proteome analysis reveals disease-resistant biomarker signatures in hybrid grouper Epinephelus fuscoguttatus a x Epinephelus lanceolatus a‚ against Vibrio alginolyticus |
| title_full_unstemmed | Skin mucus proteome analysis reveals disease-resistant biomarker signatures in hybrid grouper Epinephelus fuscoguttatus a x Epinephelus lanceolatus a‚ against Vibrio alginolyticus |
| title_short | Skin mucus proteome analysis reveals disease-resistant biomarker signatures in hybrid grouper Epinephelus fuscoguttatus a x Epinephelus lanceolatus a‚ against Vibrio alginolyticus |
| title_sort | skin mucus proteome analysis reveals disease resistant biomarker signatures in hybrid grouper epinephelus fuscoguttatus a x epinephelus lanceolatus a against vibrio alginolyticus |
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