Synthesis, characterization, and antimicrobial and antiproliferative effects of CuO-TiO2-chitosan-escin nanocomposites on human leukemic MOLT4 cells
Nanocomposites comprised of CuO-TiO2-chitosan-escin, which has adjustable physicochemical properties, provide a solution for therapeutic selectivity in cancer treatment. By controlling the intrinsic signaling primarily through the mitochondrial signaling pathway, we desired nanocomposites with enhan...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Multidisciplinary Digital Publishing Institute
2022
|
| _version_ | 1825938844638248960 |
|---|---|
| author | Elderdery, Abozer Y. Alhamidi, Abdulaziz H. Elkhalifa, Ahmed M. E. Althobiti, Maryam M. Omer, Nawal Eltayeb Alsugoor, Mahdi H. Alsuhaymi, Naif Atebien, Entesar M. Hamza, Siddiqa M. A. Alzahrani, Badr Alanazi, Fehaid Kumar, Suresh S. Mok, Pooi Ling |
| author_facet | Elderdery, Abozer Y. Alhamidi, Abdulaziz H. Elkhalifa, Ahmed M. E. Althobiti, Maryam M. Omer, Nawal Eltayeb Alsugoor, Mahdi H. Alsuhaymi, Naif Atebien, Entesar M. Hamza, Siddiqa M. A. Alzahrani, Badr Alanazi, Fehaid Kumar, Suresh S. Mok, Pooi Ling |
| author_sort | Elderdery, Abozer Y. |
| collection | UPM |
| description | Nanocomposites comprised of CuO-TiO2-chitosan-escin, which has adjustable physicochemical properties, provide a solution for therapeutic selectivity in cancer treatment. By controlling the intrinsic signaling primarily through the mitochondrial signaling pathway, we desired nanocomposites with enhanced anticancer activity by containing CuO-TiO2-chitosan-escin. The metal oxides CuO and TiO2, the natural polymer chitosan, and a phytochemical compound escin were combined to form CuO-TiO2-chitosan-escin nanocomposites. The synthesized nanocomposites were confirmed and characterized using FTIR spectroscopy, TEM, and UV-Vis absorption spectroscopy. A human leukemia cell line (MOLT-4) was used to assess the efficacy and selectivity of nanocomposites. Based on a cytotoxicity study, CuO-TiO2-chitosan-escin nanocomposites had inhibition concentrations (IC50) of 13.68, 8.9, and 7.14 µg/mL against human T lymphoblast cells after 24, 48, and 72 h of incubation, respectively. Compared with untreated MOLT-4 cells, CuO-TiO2-chitosan-escin nanocomposite-treated cells significantly increased (p < 0.05) caspase-3, -8, and -9 and decreased the levels of antioxidant enzymes GR, SOD, and GSH. Furthermore, MDA for lipid peroxidase and ROS levels significantly increased (p < 0.05) in the treated cells than in the untreated cells. Remarkably, CuO-TiO2-chitosan-escin nanocomposite-mediated control of cell cycles were mainly achieved through the activation of caspase-3, -8, and -9. |
| first_indexed | 2024-03-06T11:18:22Z |
| format | Article |
| id | upm.eprints-103398 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:18:22Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-1033982023-06-13T03:32:11Z http://psasir.upm.edu.my/id/eprint/103398/ Synthesis, characterization, and antimicrobial and antiproliferative effects of CuO-TiO2-chitosan-escin nanocomposites on human leukemic MOLT4 cells Elderdery, Abozer Y. Alhamidi, Abdulaziz H. Elkhalifa, Ahmed M. E. Althobiti, Maryam M. Omer, Nawal Eltayeb Alsugoor, Mahdi H. Alsuhaymi, Naif Atebien, Entesar M. Hamza, Siddiqa M. A. Alzahrani, Badr Alanazi, Fehaid Kumar, Suresh S. Mok, Pooi Ling Nanocomposites comprised of CuO-TiO2-chitosan-escin, which has adjustable physicochemical properties, provide a solution for therapeutic selectivity in cancer treatment. By controlling the intrinsic signaling primarily through the mitochondrial signaling pathway, we desired nanocomposites with enhanced anticancer activity by containing CuO-TiO2-chitosan-escin. The metal oxides CuO and TiO2, the natural polymer chitosan, and a phytochemical compound escin were combined to form CuO-TiO2-chitosan-escin nanocomposites. The synthesized nanocomposites were confirmed and characterized using FTIR spectroscopy, TEM, and UV-Vis absorption spectroscopy. A human leukemia cell line (MOLT-4) was used to assess the efficacy and selectivity of nanocomposites. Based on a cytotoxicity study, CuO-TiO2-chitosan-escin nanocomposites had inhibition concentrations (IC50) of 13.68, 8.9, and 7.14 µg/mL against human T lymphoblast cells after 24, 48, and 72 h of incubation, respectively. Compared with untreated MOLT-4 cells, CuO-TiO2-chitosan-escin nanocomposite-treated cells significantly increased (p < 0.05) caspase-3, -8, and -9 and decreased the levels of antioxidant enzymes GR, SOD, and GSH. Furthermore, MDA for lipid peroxidase and ROS levels significantly increased (p < 0.05) in the treated cells than in the untreated cells. Remarkably, CuO-TiO2-chitosan-escin nanocomposite-mediated control of cell cycles were mainly achieved through the activation of caspase-3, -8, and -9. Multidisciplinary Digital Publishing Institute 2022 Article PeerReviewed Elderdery, Abozer Y. and Alhamidi, Abdulaziz H. and Elkhalifa, Ahmed M. E. and Althobiti, Maryam M. and Omer, Nawal Eltayeb and Alsugoor, Mahdi H. and Alsuhaymi, Naif and Atebien, Entesar M. and Hamza, Siddiqa M. A. and Alzahrani, Badr and Alanazi, Fehaid and Kumar, Suresh S. and Mok, Pooi Ling (2022) Synthesis, characterization, and antimicrobial and antiproliferative effects of CuO-TiO2-chitosan-escin nanocomposites on human leukemic MOLT4 cells. Nanomaterials, 12 (21). art. no. 3753. pp. 1-12. ISSN 2079-4991 https://www.mdpi.com/2079-4991/12/21/3753 10.3390/nano12213753 |
| spellingShingle | Elderdery, Abozer Y. Alhamidi, Abdulaziz H. Elkhalifa, Ahmed M. E. Althobiti, Maryam M. Omer, Nawal Eltayeb Alsugoor, Mahdi H. Alsuhaymi, Naif Atebien, Entesar M. Hamza, Siddiqa M. A. Alzahrani, Badr Alanazi, Fehaid Kumar, Suresh S. Mok, Pooi Ling Synthesis, characterization, and antimicrobial and antiproliferative effects of CuO-TiO2-chitosan-escin nanocomposites on human leukemic MOLT4 cells |
| title | Synthesis, characterization, and antimicrobial and antiproliferative effects of CuO-TiO2-chitosan-escin nanocomposites on human leukemic MOLT4 cells |
| title_full | Synthesis, characterization, and antimicrobial and antiproliferative effects of CuO-TiO2-chitosan-escin nanocomposites on human leukemic MOLT4 cells |
| title_fullStr | Synthesis, characterization, and antimicrobial and antiproliferative effects of CuO-TiO2-chitosan-escin nanocomposites on human leukemic MOLT4 cells |
| title_full_unstemmed | Synthesis, characterization, and antimicrobial and antiproliferative effects of CuO-TiO2-chitosan-escin nanocomposites on human leukemic MOLT4 cells |
| title_short | Synthesis, characterization, and antimicrobial and antiproliferative effects of CuO-TiO2-chitosan-escin nanocomposites on human leukemic MOLT4 cells |
| title_sort | synthesis characterization and antimicrobial and antiproliferative effects of cuo tio2 chitosan escin nanocomposites on human leukemic molt4 cells |
| work_keys_str_mv | AT elderderyabozery synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT alhamidiabdulazizh synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT elkhalifaahmedme synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT althobitimaryamm synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT omernawaleltayeb synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT alsugoormahdih synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT alsuhayminaif synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT atebienentesarm synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT hamzasiddiqama synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT alzahranibadr synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT alanazifehaid synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT kumarsureshs synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells AT mokpooiling synthesischaracterizationandantimicrobialandantiproliferativeeffectsofcuotio2chitosanescinnanocompositesonhumanleukemicmolt4cells |