Cultivation of grey oyster mushroom [Pleurotus pulmonarius (Fr.) Quél.] on different alkaline materials and its antioxidant properties
The study aims to ascertain the effect of different alkaline materials’ supplementation on mushroom spawn, yield and antioxidant properties of grey oyster mushroom. The mushroom spawn grown on wheat with the treatment of zeolite (0.5%, 1%, 2%) and gypsum (1%, 2%) showed the rapid growth in spawnr...
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| Format: | Thesis |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/104459/1/MUHAMAD%20FAKHRUL%20RADZI%20-%20IR.pdf |
| _version_ | 1825939042937602048 |
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| author | Muhamad Pikri, Muhamad Fakhrul Radzi |
| author_facet | Muhamad Pikri, Muhamad Fakhrul Radzi |
| author_sort | Muhamad Pikri, Muhamad Fakhrul Radzi |
| collection | UPM |
| description | The study aims to ascertain the effect of different alkaline materials’
supplementation on mushroom spawn, yield and antioxidant properties of grey
oyster mushroom. The mushroom spawn grown on wheat with the treatment of
zeolite (0.5%, 1%, 2%) and gypsum (1%, 2%) showed the rapid growth in spawnrunning
time (10 days). The maize substrate coupled with all of the treatments
and the wheat substrate with gypsum (1%, 2%) developed the thickest spawn
mycelia. The antagonistic activity showed the wheat substrate with the treatment
of zeolite (2%), and the maize substrate with zeolite (2%) exhibited a high growth
inhibition rate against Trichoderma koningii with 91.67 ± 5.77 % and 90 ± 8.66
%, respectively. DNA analysis of mushroom pathogenic fungi proved the isolate
of Trichoderma sp. was genetically similar with Trichoderma koningii at a
bootstrap value of 97%. The cultivation of grey oyster mushrooms between
different alkaline treatments was resolved to show the significant effect on
mushroom production. The substrate in the presence of gypsum took minimum
days for mycelial growth (30.7 ± 4.12 days) and the emergence of primordia (7.7
± 4.55 days). The mushroom yield indicated that zeolite treatment showed the
most significant yield (62.36 ± 9.67 g) as collected from the first until the fourth
flush. The biological efficiency resembled by the treatment of zeolite stood out
all the treatments by 26.05 ± 11.18 %. A maximum inhibitory effect against 2,2-
diphenyl-1-picrylhydrazyl (DPPH) free radicals, IC50, was demonstrated by
zeolite treatment at the third flush (63.48 ± 28.804 μg/mL). The fruiting body
produced from the zeolite treatment was highly containing phenolic compound
(98.96 ± 10.07 μg GAE/mg), as noticed in the fourth flush. Therefore,
supplementation of gypsum and zeolite in substrate formulation might be a great
benefit to practice in promoting and increasing the quality of spawn and fruiting
body of mushrooms. |
| first_indexed | 2024-03-06T11:21:21Z |
| format | Thesis |
| id | upm.eprints-104459 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T11:21:21Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | upm.eprints-1044592023-08-17T08:02:57Z http://psasir.upm.edu.my/id/eprint/104459/ Cultivation of grey oyster mushroom [Pleurotus pulmonarius (Fr.) Quél.] on different alkaline materials and its antioxidant properties Muhamad Pikri, Muhamad Fakhrul Radzi The study aims to ascertain the effect of different alkaline materials’ supplementation on mushroom spawn, yield and antioxidant properties of grey oyster mushroom. The mushroom spawn grown on wheat with the treatment of zeolite (0.5%, 1%, 2%) and gypsum (1%, 2%) showed the rapid growth in spawnrunning time (10 days). The maize substrate coupled with all of the treatments and the wheat substrate with gypsum (1%, 2%) developed the thickest spawn mycelia. The antagonistic activity showed the wheat substrate with the treatment of zeolite (2%), and the maize substrate with zeolite (2%) exhibited a high growth inhibition rate against Trichoderma koningii with 91.67 ± 5.77 % and 90 ± 8.66 %, respectively. DNA analysis of mushroom pathogenic fungi proved the isolate of Trichoderma sp. was genetically similar with Trichoderma koningii at a bootstrap value of 97%. The cultivation of grey oyster mushrooms between different alkaline treatments was resolved to show the significant effect on mushroom production. The substrate in the presence of gypsum took minimum days for mycelial growth (30.7 ± 4.12 days) and the emergence of primordia (7.7 ± 4.55 days). The mushroom yield indicated that zeolite treatment showed the most significant yield (62.36 ± 9.67 g) as collected from the first until the fourth flush. The biological efficiency resembled by the treatment of zeolite stood out all the treatments by 26.05 ± 11.18 %. A maximum inhibitory effect against 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radicals, IC50, was demonstrated by zeolite treatment at the third flush (63.48 ± 28.804 μg/mL). The fruiting body produced from the zeolite treatment was highly containing phenolic compound (98.96 ± 10.07 μg GAE/mg), as noticed in the fourth flush. Therefore, supplementation of gypsum and zeolite in substrate formulation might be a great benefit to practice in promoting and increasing the quality of spawn and fruiting body of mushrooms. 2022-07 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/104459/1/MUHAMAD%20FAKHRUL%20RADZI%20-%20IR.pdf Muhamad Pikri, Muhamad Fakhrul Radzi (2022) Cultivation of grey oyster mushroom [Pleurotus pulmonarius (Fr.) Quél.] on different alkaline materials and its antioxidant properties. Masters thesis, Universiti Putra Malaysia. Mushrooms Plant - Effect of chemicals on |
| spellingShingle | Mushrooms Plant - Effect of chemicals on Muhamad Pikri, Muhamad Fakhrul Radzi Cultivation of grey oyster mushroom [Pleurotus pulmonarius (Fr.) Quél.] on different alkaline materials and its antioxidant properties |
| title | Cultivation of grey oyster mushroom [Pleurotus pulmonarius (Fr.) Quél.] on different alkaline materials and its antioxidant properties |
| title_full | Cultivation of grey oyster mushroom [Pleurotus pulmonarius (Fr.) Quél.] on different alkaline materials and its antioxidant properties |
| title_fullStr | Cultivation of grey oyster mushroom [Pleurotus pulmonarius (Fr.) Quél.] on different alkaline materials and its antioxidant properties |
| title_full_unstemmed | Cultivation of grey oyster mushroom [Pleurotus pulmonarius (Fr.) Quél.] on different alkaline materials and its antioxidant properties |
| title_short | Cultivation of grey oyster mushroom [Pleurotus pulmonarius (Fr.) Quél.] on different alkaline materials and its antioxidant properties |
| title_sort | cultivation of grey oyster mushroom pleurotus pulmonarius fr quel on different alkaline materials and its antioxidant properties |
| topic | Mushrooms Plant - Effect of chemicals on |
| url | http://psasir.upm.edu.my/id/eprint/104459/1/MUHAMAD%20FAKHRUL%20RADZI%20-%20IR.pdf |
| work_keys_str_mv | AT muhamadpikrimuhamadfakhrulradzi cultivationofgreyoystermushroompleurotuspulmonariusfrquelondifferentalkalinematerialsanditsantioxidantproperties |