Diesel−biodegradation and biosurfactant−production by Janthinobacterium lividum AQ5-29 and Pseudomonas fildesensis AQ5-41 isolated from Antarctic soil
Given the substantial diesel demand in Antarctic operations, the means of addressing ecological restoration following its inappropriate release are attracting attention from researchers. The Madrid Protocol mandates the use of indigenous microbes in bioremediation. Recent studies have proposed many...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Elsevier
2024
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| _version_ | 1811137665584594944 |
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| author | Yap, How Swen Khalid, Farah Eryssa Wong, Rasidnie Razin Convey, Peter Sabri, Suriana Khalil, Khalilah Abdul Zulkharnain, Azham Merican, Faradina Shaari, Hasrizal Ahmad, Siti Aqlima |
| author_facet | Yap, How Swen Khalid, Farah Eryssa Wong, Rasidnie Razin Convey, Peter Sabri, Suriana Khalil, Khalilah Abdul Zulkharnain, Azham Merican, Faradina Shaari, Hasrizal Ahmad, Siti Aqlima |
| author_sort | Yap, How Swen |
| collection | UPM |
| description | Given the substantial diesel demand in Antarctic operations, the means of addressing ecological restoration following its inappropriate release are attracting attention from researchers. The Madrid Protocol mandates the use of indigenous microbes in bioremediation. Recent studies have proposed many native isolates with biodegradation temperatures exceeding 20 °C, which are impractical for Antarctic contexts. Therefore, harnessing psychrophilic, native degraders with biosurfactant−producing traits presents an advantage for implementation in the harsh Antarctic environment. In this study, effective consortia/isolates demonstrated robust growth and biodegradation rates at 10 °C with diesel as the sole carbon source. Two primary bacterial members, Janthinobacterium lividum and Pseudomonas fildesensis, were identified from the most effective consortium SI 20 using 16S rRNA and multilocus−sequence−analysis (MLSA) clustering. The degraders were characterised as being psychrophilic, Gram-negative, rod−shaped, and catalase− and oxidase−positive. Despite the observed antagonistic effects during co-cultivation, strains J. lividum AQ5-29 and P. fildesensis AQ5-41 demonstrated effective diesel removal (2.91 & 4.20 mg mL−1) with biodegradation of C10 to C30 hydrocarbons (40−100) at 10 °C in less than 8 days. Both strains also were identified as biosurfactant producers with varying emulsification activities (32−92) and cell surface hydrophobicity (52−58). These findings highlight the potential of both strains for restoring diesel-related substrates, particularly in Antarctica. |
| first_indexed | 2024-09-25T03:37:55Z |
| format | Article |
| id | upm.eprints-105610 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:37:55Z |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-1056102024-05-06T07:34:14Z http://psasir.upm.edu.my/id/eprint/105610/ Diesel−biodegradation and biosurfactant−production by Janthinobacterium lividum AQ5-29 and Pseudomonas fildesensis AQ5-41 isolated from Antarctic soil Yap, How Swen Khalid, Farah Eryssa Wong, Rasidnie Razin Convey, Peter Sabri, Suriana Khalil, Khalilah Abdul Zulkharnain, Azham Merican, Faradina Shaari, Hasrizal Ahmad, Siti Aqlima Given the substantial diesel demand in Antarctic operations, the means of addressing ecological restoration following its inappropriate release are attracting attention from researchers. The Madrid Protocol mandates the use of indigenous microbes in bioremediation. Recent studies have proposed many native isolates with biodegradation temperatures exceeding 20 °C, which are impractical for Antarctic contexts. Therefore, harnessing psychrophilic, native degraders with biosurfactant−producing traits presents an advantage for implementation in the harsh Antarctic environment. In this study, effective consortia/isolates demonstrated robust growth and biodegradation rates at 10 °C with diesel as the sole carbon source. Two primary bacterial members, Janthinobacterium lividum and Pseudomonas fildesensis, were identified from the most effective consortium SI 20 using 16S rRNA and multilocus−sequence−analysis (MLSA) clustering. The degraders were characterised as being psychrophilic, Gram-negative, rod−shaped, and catalase− and oxidase−positive. Despite the observed antagonistic effects during co-cultivation, strains J. lividum AQ5-29 and P. fildesensis AQ5-41 demonstrated effective diesel removal (2.91 & 4.20 mg mL−1) with biodegradation of C10 to C30 hydrocarbons (40−100) at 10 °C in less than 8 days. Both strains also were identified as biosurfactant producers with varying emulsification activities (32−92) and cell surface hydrophobicity (52−58). These findings highlight the potential of both strains for restoring diesel-related substrates, particularly in Antarctica. Elsevier 2024-03 Article PeerReviewed Yap, How Swen and Khalid, Farah Eryssa and Wong, Rasidnie Razin and Convey, Peter and Sabri, Suriana and Khalil, Khalilah Abdul and Zulkharnain, Azham and Merican, Faradina and Shaari, Hasrizal and Ahmad, Siti Aqlima (2024) Diesel−biodegradation and biosurfactant−production by Janthinobacterium lividum AQ5-29 and Pseudomonas fildesensis AQ5-41 isolated from Antarctic soil. International Biodeterioration and Biodegradation, 188. art. no. 105731. pp. 1-12. ISSN 0964-8305 https://linkinghub.elsevier.com/retrieve/pii/S0964830524000027 10.1016/j.ibiod.2024.105731 |
| spellingShingle | Yap, How Swen Khalid, Farah Eryssa Wong, Rasidnie Razin Convey, Peter Sabri, Suriana Khalil, Khalilah Abdul Zulkharnain, Azham Merican, Faradina Shaari, Hasrizal Ahmad, Siti Aqlima Diesel−biodegradation and biosurfactant−production by Janthinobacterium lividum AQ5-29 and Pseudomonas fildesensis AQ5-41 isolated from Antarctic soil |
| title | Diesel−biodegradation and biosurfactant−production by Janthinobacterium lividum AQ5-29 and Pseudomonas fildesensis AQ5-41 isolated from Antarctic soil |
| title_full | Diesel−biodegradation and biosurfactant−production by Janthinobacterium lividum AQ5-29 and Pseudomonas fildesensis AQ5-41 isolated from Antarctic soil |
| title_fullStr | Diesel−biodegradation and biosurfactant−production by Janthinobacterium lividum AQ5-29 and Pseudomonas fildesensis AQ5-41 isolated from Antarctic soil |
| title_full_unstemmed | Diesel−biodegradation and biosurfactant−production by Janthinobacterium lividum AQ5-29 and Pseudomonas fildesensis AQ5-41 isolated from Antarctic soil |
| title_short | Diesel−biodegradation and biosurfactant−production by Janthinobacterium lividum AQ5-29 and Pseudomonas fildesensis AQ5-41 isolated from Antarctic soil |
| title_sort | diesel biodegradation and biosurfactant production by janthinobacterium lividum aq5 29 and pseudomonas fildesensis aq5 41 isolated from antarctic soil |
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