Step-by-step approach: stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system
In vivo extracellular field potential recording is a commonly used technique in modern neuro- science research. The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery. However, there is limited use of visually guided stereotaxic neurosurgery...
| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado: |
Elsevier
2024
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| Acceso en liña: | http://psasir.upm.edu.my/id/eprint/105647/1/1-s2.0-S221501612300540X-main.pdf |
| _version_ | 1825939231003901952 |
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| author | Azimzadeh, Mansour Mohd Azmi, Mohd Amirul Najwa Reisi, Parham Cheah, Pike-See Ling, King-Hwa |
| author_facet | Azimzadeh, Mansour Mohd Azmi, Mohd Amirul Najwa Reisi, Parham Cheah, Pike-See Ling, King-Hwa |
| author_sort | Azimzadeh, Mansour |
| collection | UPM |
| description | In vivo extracellular field potential recording is a commonly used technique in modern neuro- science research. The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery. However, there is limited use of visually guided stereotaxic neurosurgery and the application of the eLab/ePulse electrophysiology system in rodent models. This study presents a practical and functional manual guide for surgical electrode implantation in rodent models using the eLab/ePulse electrophysiology system for recording and stimulation pur- poses to assess neuronal functionality and synaptic plasticity. The evaluation parameters included the input/output function (IO), paired-pulse facilitation or depression (PPF/PPD), long-term po- tentiation (LTP), and long-term depression (LTD). |
| first_indexed | 2024-09-25T03:38:01Z |
| format | Article |
| id | upm.eprints-105647 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-09-25T03:38:01Z |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-1056472024-06-21T02:34:29Z http://psasir.upm.edu.my/id/eprint/105647/ Step-by-step approach: stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system Azimzadeh, Mansour Mohd Azmi, Mohd Amirul Najwa Reisi, Parham Cheah, Pike-See Ling, King-Hwa In vivo extracellular field potential recording is a commonly used technique in modern neuro- science research. The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery. However, there is limited use of visually guided stereotaxic neurosurgery and the application of the eLab/ePulse electrophysiology system in rodent models. This study presents a practical and functional manual guide for surgical electrode implantation in rodent models using the eLab/ePulse electrophysiology system for recording and stimulation pur- poses to assess neuronal functionality and synaptic plasticity. The evaluation parameters included the input/output function (IO), paired-pulse facilitation or depression (PPF/PPD), long-term po- tentiation (LTP), and long-term depression (LTD). Elsevier 2024-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/105647/1/1-s2.0-S221501612300540X-main.pdf Azimzadeh, Mansour and Mohd Azmi, Mohd Amirul Najwa and Reisi, Parham and Cheah, Pike-See and Ling, King-Hwa (2024) Step-by-step approach: stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system. MethodsX, 12. art. no. 102544. pp. 1-13. ISSN 2215-0161 https://linkinghub.elsevier.com/retrieve/pii/S221501612300540X 10.1016/j.mex.2023.102544 |
| spellingShingle | Azimzadeh, Mansour Mohd Azmi, Mohd Amirul Najwa Reisi, Parham Cheah, Pike-See Ling, King-Hwa Step-by-step approach: stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system |
| title | Step-by-step approach: stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system |
| title_full | Step-by-step approach: stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system |
| title_fullStr | Step-by-step approach: stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system |
| title_full_unstemmed | Step-by-step approach: stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system |
| title_short | Step-by-step approach: stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system |
| title_sort | step by step approach stereotaxic surgery for in vivo extracellular field potential recording at the rat schaffer collateral ca1 synapse using the elab system |
| url | http://psasir.upm.edu.my/id/eprint/105647/1/1-s2.0-S221501612300540X-main.pdf |
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