NMR-based metabolomics and UHPLC-ESI-MS/MS profiling of Syzygium jambos in relation to their antioxidant and anti-hyperglycemic activities
Search for natural sources in disease management especially diabetes has surged recently for its potential health benefits. However, several fruit tree species remained unpopular despite their folkloric usage due to insufficient scientific data. Syzygium jambos (L.) Alston (Myrtaceae) is a fruit-bea...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/105661/1/1-s2.0-S1878535223010080-main.pdf |
| _version_ | 1811137674578231296 |
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| author | Wong, Pei Lou Ramli, Nurul Shazini Tan, Chin Ping Azlan, Azrina Abas, Faridah |
| author_facet | Wong, Pei Lou Ramli, Nurul Shazini Tan, Chin Ping Azlan, Azrina Abas, Faridah |
| author_sort | Wong, Pei Lou |
| collection | UPM |
| description | Search for natural sources in disease management especially diabetes has surged recently for its potential health benefits. However, several fruit tree species remained unpopular despite their folkloric usage due to insufficient scientific data. Syzygium jambos (L.) Alston (Myrtaceae) is a fruit-bearing plant that is used as a traditional dietary supplement to treat various diseases including diabetes. This study investigated the correlation between S. jambos leaves’ metabolites and the bioactivities using proton-nuclear magnetic resonance (1H NMR)-based metabolomics approach. In-vitro total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radicals scavenging, nitric oxide (NO•) radicals scavenging, anti-α-amylase and anti-α-glucosidase assays were performed. The findings indicated that 70 ethanolic extract demonstrated the highest potential in overall bioactivities. A total of 59 and 30 metabolites were tentatively identified using ultrahigh-performance liquid chromatography electrospray ionization Quadrupole-Orbitrap tandem mass spectrometry (UHPLC/ESI Q-Orbitrap MS/MS) and NMR, respectively. The partial least square (PLS) model revealed tannins, triterpenoids, and flavonoids significantly contributed to the separation and bioactivities. This study provides comprehensive insights into S. jambos metabolome and reveals the potential as a reliable source of antioxidant and anti-hyperglycemic compounds. © 2023 The Authors |
| first_indexed | 2024-03-06T11:23:18Z |
| format | Article |
| id | upm.eprints-105661 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-09-25T03:38:03Z |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-1056612024-07-10T04:32:14Z http://psasir.upm.edu.my/id/eprint/105661/ NMR-based metabolomics and UHPLC-ESI-MS/MS profiling of Syzygium jambos in relation to their antioxidant and anti-hyperglycemic activities Wong, Pei Lou Ramli, Nurul Shazini Tan, Chin Ping Azlan, Azrina Abas, Faridah Search for natural sources in disease management especially diabetes has surged recently for its potential health benefits. However, several fruit tree species remained unpopular despite their folkloric usage due to insufficient scientific data. Syzygium jambos (L.) Alston (Myrtaceae) is a fruit-bearing plant that is used as a traditional dietary supplement to treat various diseases including diabetes. This study investigated the correlation between S. jambos leaves’ metabolites and the bioactivities using proton-nuclear magnetic resonance (1H NMR)-based metabolomics approach. In-vitro total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radicals scavenging, nitric oxide (NO•) radicals scavenging, anti-α-amylase and anti-α-glucosidase assays were performed. The findings indicated that 70 ethanolic extract demonstrated the highest potential in overall bioactivities. A total of 59 and 30 metabolites were tentatively identified using ultrahigh-performance liquid chromatography electrospray ionization Quadrupole-Orbitrap tandem mass spectrometry (UHPLC/ESI Q-Orbitrap MS/MS) and NMR, respectively. The partial least square (PLS) model revealed tannins, triterpenoids, and flavonoids significantly contributed to the separation and bioactivities. This study provides comprehensive insights into S. jambos metabolome and reveals the potential as a reliable source of antioxidant and anti-hyperglycemic compounds. © 2023 The Authors Elsevier 2024-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/105661/1/1-s2.0-S1878535223010080-main.pdf Wong, Pei Lou and Ramli, Nurul Shazini and Tan, Chin Ping and Azlan, Azrina and Abas, Faridah (2024) NMR-based metabolomics and UHPLC-ESI-MS/MS profiling of Syzygium jambos in relation to their antioxidant and anti-hyperglycemic activities. Arabian Journal of Chemistry, 17 (2). art. no. 105546. pp. 1-13. ISSN 1878-5352 https://www.sciencedirect.com/science/article/pii/S1878535223010080 10.1016/j.arabjc.2023.105546 |
| spellingShingle | Wong, Pei Lou Ramli, Nurul Shazini Tan, Chin Ping Azlan, Azrina Abas, Faridah NMR-based metabolomics and UHPLC-ESI-MS/MS profiling of Syzygium jambos in relation to their antioxidant and anti-hyperglycemic activities |
| title | NMR-based metabolomics and UHPLC-ESI-MS/MS profiling of Syzygium jambos in relation to their antioxidant and anti-hyperglycemic activities |
| title_full | NMR-based metabolomics and UHPLC-ESI-MS/MS profiling of Syzygium jambos in relation to their antioxidant and anti-hyperglycemic activities |
| title_fullStr | NMR-based metabolomics and UHPLC-ESI-MS/MS profiling of Syzygium jambos in relation to their antioxidant and anti-hyperglycemic activities |
| title_full_unstemmed | NMR-based metabolomics and UHPLC-ESI-MS/MS profiling of Syzygium jambos in relation to their antioxidant and anti-hyperglycemic activities |
| title_short | NMR-based metabolomics and UHPLC-ESI-MS/MS profiling of Syzygium jambos in relation to their antioxidant and anti-hyperglycemic activities |
| title_sort | nmr based metabolomics and uhplc esi ms ms profiling of syzygium jambos in relation to their antioxidant and anti hyperglycemic activities |
| url | http://psasir.upm.edu.my/id/eprint/105661/1/1-s2.0-S1878535223010080-main.pdf |
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