Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes)
Phthalate and non-phthalate plasticizers are used in polymer materials, such as plastic and rubber. It has recently been found that diisobutyl adipate (DIBA), which is considered an environmentally safe non-phthalate plasticizer, potentially acts as a thyroid disruptor in fish. Here, we investigated...
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Elsevier
2024
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author | Horie, Yoshifumi Chihaya, Yuto Yap, Chee Kong Ríos, Juan Manuel Ramaswamy, Babu Rajendran Uaciquete, Dorcas |
author_facet | Horie, Yoshifumi Chihaya, Yuto Yap, Chee Kong Ríos, Juan Manuel Ramaswamy, Babu Rajendran Uaciquete, Dorcas |
author_sort | Horie, Yoshifumi |
collection | UPM |
description | Phthalate and non-phthalate plasticizers are used in polymer materials, such as plastic and rubber. It has recently been found that diisobutyl adipate (DIBA), which is considered an environmentally safe non-phthalate plasticizer, potentially acts as a thyroid disruptor in fish. Here, we investigated the sexual hormone effects of DIBA based on the expression levels of genes that respond to endocrine disruption and sexual hormone activity in the livers and gonads, and on gonadal sexual differentiation in Japanese medaka. Compared with the control group, the mRNA expression of chgH, vtg1, vtg2, and esr1 was significantly suppressed in the livers of DIBA exposed XX individuals. Furthermore, the mRNA expression of gsdf was significantly upregulated and downregulated in the gonads of XX and XY individuals, respectively. The mRNA expressions of esr1 and esr2b were significantly suppressed by DIBA exposure in the gonads of both XX and XY individuals. These observations suggest that DIBA has potential androgenic activity in Japanese medaka. However, normal testes and ovaries were observed in respective XY and XX medaka after DIBA exposure; therefore, these results suggest that DIBA may have weak androgenic activity. © 2024 Elsevier Inc. |
first_indexed | 2024-03-06T11:23:19Z |
format | Article |
id | upm.eprints-105668 |
institution | Universiti Putra Malaysia |
last_indexed | 2024-03-06T11:23:19Z |
publishDate | 2024 |
publisher | Elsevier |
record_format | dspace |
spelling | upm.eprints-1056682024-02-08T03:08:00Z http://psasir.upm.edu.my/id/eprint/105668/ Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes) Horie, Yoshifumi Chihaya, Yuto Yap, Chee Kong Ríos, Juan Manuel Ramaswamy, Babu Rajendran Uaciquete, Dorcas Phthalate and non-phthalate plasticizers are used in polymer materials, such as plastic and rubber. It has recently been found that diisobutyl adipate (DIBA), which is considered an environmentally safe non-phthalate plasticizer, potentially acts as a thyroid disruptor in fish. Here, we investigated the sexual hormone effects of DIBA based on the expression levels of genes that respond to endocrine disruption and sexual hormone activity in the livers and gonads, and on gonadal sexual differentiation in Japanese medaka. Compared with the control group, the mRNA expression of chgH, vtg1, vtg2, and esr1 was significantly suppressed in the livers of DIBA exposed XX individuals. Furthermore, the mRNA expression of gsdf was significantly upregulated and downregulated in the gonads of XX and XY individuals, respectively. The mRNA expressions of esr1 and esr2b were significantly suppressed by DIBA exposure in the gonads of both XX and XY individuals. These observations suggest that DIBA has potential androgenic activity in Japanese medaka. However, normal testes and ovaries were observed in respective XY and XX medaka after DIBA exposure; therefore, these results suggest that DIBA may have weak androgenic activity. © 2024 Elsevier Inc. Elsevier 2024 Article PeerReviewed Horie, Yoshifumi and Chihaya, Yuto and Yap, Chee Kong and Ríos, Juan Manuel and Ramaswamy, Babu Rajendran and Uaciquete, Dorcas (2024) Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes). Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 277. art. no. 109836. pp. 1-8. ISSN 1532-0456 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85182690193&doi=10.1016%2fj.cbpc.2024.109836&partnerID=40&md5=16f7c5b599a7251bc5ef6cd0bf526f2b 10.1016/j.cbpc.2024.109836 |
spellingShingle | Horie, Yoshifumi Chihaya, Yuto Yap, Chee Kong Ríos, Juan Manuel Ramaswamy, Babu Rajendran Uaciquete, Dorcas Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes) |
title | Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes) |
title_full | Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes) |
title_fullStr | Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes) |
title_full_unstemmed | Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes) |
title_short | Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes) |
title_sort | effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in japanese medaka oryzias latipes |
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