Mangifera indica L. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells
In this study, we investigated the effects of an ethanolic extract of Mangifera indica L. kernel on the viability and proliferation of human lung cancer cells. We utilized MTT and BrdU cell proliferation assays, morphological assessments, cell cycle analyses, and apoptosis assays to investigate the...
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| Format: | Article |
| Language: | English |
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Taylor and Francis
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/105717/1/Mangifera%20indica%20L.%20kernel%20ethanol%20extract%20inhibits%20cell%20viability%20and%20proliferation%20with%20induction%20of%20cell%20cycle%20arrest%20and%20apoptosis%20in%20lung%20cancer%20.pdf |
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| author | Alshwyeh, Hussah Abdullah Al-Sheikh, Warqaa Muhammed Shariff Rasedee, Abdullah Alnasser, Sulaiman Mohammed Al-Qubaisi, Mothanna Sadiq Ibrahim, Wisam Nabeel |
| author_facet | Alshwyeh, Hussah Abdullah Al-Sheikh, Warqaa Muhammed Shariff Rasedee, Abdullah Alnasser, Sulaiman Mohammed Al-Qubaisi, Mothanna Sadiq Ibrahim, Wisam Nabeel |
| author_sort | Alshwyeh, Hussah Abdullah |
| collection | UPM |
| description | In this study, we investigated the effects of an ethanolic extract of Mangifera indica L. kernel on the viability and proliferation of human lung cancer cells. We utilized MTT and BrdU cell proliferation assays, morphological assessments, cell cycle analyses, and apoptosis assays to investigate the extract’s effects on lung cancer (A549 and NCI-H292) and normal lung (MRC-5) cells. The extract demonstrated a toxicity toward cancer cells compared to normal cells with dose-dependent anti-proliferative effect on lung cancer cells. The extract also caused differential effects on the cell cycle, inducing G0/G1 arrest and increasing the Sub-G1 population in both lung cancer and normal lung cells. Notably, the extract induced loss of membrane integrity, shrinkage, membrane blebbing, and apoptosis in lung cancer cells, while normal cells exhibited only early apoptosis. Furthermore, the extract exhibited higher toxicity towards NCI-H292 cells, followed by A549 and normal MRC-5 cells in decreasing order of potency. Our results suggest that the ethanolic extract of M. indica L. kernel has significant potential as a novel therapeutic agent for treating lung cancer cells, given its ability to induce apoptosis in cancer cell lines while causing minimal harm to normal cells. |
| first_indexed | 2024-03-06T11:23:28Z |
| format | Article |
| id | upm.eprints-105717 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-09-25T03:38:08Z |
| publishDate | 2024 |
| publisher | Taylor and Francis |
| record_format | dspace |
| spelling | upm.eprints-1057172024-07-13T03:58:22Z http://psasir.upm.edu.my/id/eprint/105717/ Mangifera indica L. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells Alshwyeh, Hussah Abdullah Al-Sheikh, Warqaa Muhammed Shariff Rasedee, Abdullah Alnasser, Sulaiman Mohammed Al-Qubaisi, Mothanna Sadiq Ibrahim, Wisam Nabeel In this study, we investigated the effects of an ethanolic extract of Mangifera indica L. kernel on the viability and proliferation of human lung cancer cells. We utilized MTT and BrdU cell proliferation assays, morphological assessments, cell cycle analyses, and apoptosis assays to investigate the extract’s effects on lung cancer (A549 and NCI-H292) and normal lung (MRC-5) cells. The extract demonstrated a toxicity toward cancer cells compared to normal cells with dose-dependent anti-proliferative effect on lung cancer cells. The extract also caused differential effects on the cell cycle, inducing G0/G1 arrest and increasing the Sub-G1 population in both lung cancer and normal lung cells. Notably, the extract induced loss of membrane integrity, shrinkage, membrane blebbing, and apoptosis in lung cancer cells, while normal cells exhibited only early apoptosis. Furthermore, the extract exhibited higher toxicity towards NCI-H292 cells, followed by A549 and normal MRC-5 cells in decreasing order of potency. Our results suggest that the ethanolic extract of M. indica L. kernel has significant potential as a novel therapeutic agent for treating lung cancer cells, given its ability to induce apoptosis in cancer cell lines while causing minimal harm to normal cells. Taylor and Francis 2024-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/105717/1/Mangifera%20indica%20L.%20kernel%20ethanol%20extract%20inhibits%20cell%20viability%20and%20proliferation%20with%20induction%20of%20cell%20cycle%20arrest%20and%20apoptosis%20in%20lung%20cancer%20.pdf Alshwyeh, Hussah Abdullah and Al-Sheikh, Warqaa Muhammed Shariff and Rasedee, Abdullah and Alnasser, Sulaiman Mohammed and Al-Qubaisi, Mothanna Sadiq and Ibrahim, Wisam Nabeel (2024) Mangifera indica L. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells. Molecular and Cellular Oncology, 11 (1). art. no. 2299046. pp. 1-11. ISSN 2372-3556 https://www.tandfonline.com/doi/full/10.1080/23723556.2023.2299046 10.1080/23723556.2023.2299046 |
| spellingShingle | Alshwyeh, Hussah Abdullah Al-Sheikh, Warqaa Muhammed Shariff Rasedee, Abdullah Alnasser, Sulaiman Mohammed Al-Qubaisi, Mothanna Sadiq Ibrahim, Wisam Nabeel Mangifera indica L. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells |
| title | Mangifera indica L. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells |
| title_full | Mangifera indica L. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells |
| title_fullStr | Mangifera indica L. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells |
| title_full_unstemmed | Mangifera indica L. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells |
| title_short | Mangifera indica L. kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells |
| title_sort | mangifera indica l kernel ethanol extract inhibits cell viability and proliferation with induction of cell cycle arrest and apoptosis in lung cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/105717/1/Mangifera%20indica%20L.%20kernel%20ethanol%20extract%20inhibits%20cell%20viability%20and%20proliferation%20with%20induction%20of%20cell%20cycle%20arrest%20and%20apoptosis%20in%20lung%20cancer%20.pdf |
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