Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform
The displacement of an electroactive monitoring agent, i.e., polyamidoamine dendrimers encapsulated gold nanoparticles (PAMAM-Au) upon the presence of a target antibody via acoustic streaming has been studied. Acoustic streaming has been used to improve the mass transfer and reduce the sample incuba...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Institute of Physics
2024
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| _version_ | 1825939259093155840 |
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| author | Syamila, Noor Hamzah, Amir Syahir Amir Laurell, Thomas Sulaiman, Yusran Ikeno, Shinya Siang Tan, Wen Ahmad Tajudin, Asilah |
| author_facet | Syamila, Noor Hamzah, Amir Syahir Amir Laurell, Thomas Sulaiman, Yusran Ikeno, Shinya Siang Tan, Wen Ahmad Tajudin, Asilah |
| author_sort | Syamila, Noor |
| collection | UPM |
| description | The displacement of an electroactive monitoring agent, i.e., polyamidoamine dendrimers encapsulated gold nanoparticles (PAMAM-Au) upon the presence of a target antibody via acoustic streaming has been studied. Acoustic streaming has been used to improve the mass transfer and reduce the sample incubation rate, thus this study investigated its ability in enhancing the PAMAM-Au displacement efficiency of our immunosensor. For this purpose, the bio-nanogate components of maltose-binding protein carrying the antigenic determinant (MBP-aD) of hepatitis B surface antigen (HBsAg) as a bioreceptor was functionalized, followed by the monitoring agent i.e. PAMAM-Au on the electrode prior to the incubation with targeted anti-hepatitis B surface antigen (anti-HBsAg) antibody. The modified electrode was then coupled with a piezotransducer and connected to the signal transducer to induce acoustic streaming upon sample incubation. Under optimal acoustic actuation, the sample incubation time has been reduced from 20 min to 8 min via the enhancement of PAMAM-Au displacement induced by acoustic streaming. The result also demonstrated that the specificity and selectivity of the sensing platform under acoustic actuation are comparable to the static incubation in detecting the targeted antibody. |
| first_indexed | 2024-04-09T03:51:36Z |
| format | Article |
| id | upm.eprints-105796 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-04-09T03:51:36Z |
| publishDate | 2024 |
| publisher | Institute of Physics |
| record_format | dspace |
| spelling | upm.eprints-1057962024-04-02T08:54:34Z http://psasir.upm.edu.my/id/eprint/105796/ Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform Syamila, Noor Hamzah, Amir Syahir Amir Laurell, Thomas Sulaiman, Yusran Ikeno, Shinya Siang Tan, Wen Ahmad Tajudin, Asilah The displacement of an electroactive monitoring agent, i.e., polyamidoamine dendrimers encapsulated gold nanoparticles (PAMAM-Au) upon the presence of a target antibody via acoustic streaming has been studied. Acoustic streaming has been used to improve the mass transfer and reduce the sample incubation rate, thus this study investigated its ability in enhancing the PAMAM-Au displacement efficiency of our immunosensor. For this purpose, the bio-nanogate components of maltose-binding protein carrying the antigenic determinant (MBP-aD) of hepatitis B surface antigen (HBsAg) as a bioreceptor was functionalized, followed by the monitoring agent i.e. PAMAM-Au on the electrode prior to the incubation with targeted anti-hepatitis B surface antigen (anti-HBsAg) antibody. The modified electrode was then coupled with a piezotransducer and connected to the signal transducer to induce acoustic streaming upon sample incubation. Under optimal acoustic actuation, the sample incubation time has been reduced from 20 min to 8 min via the enhancement of PAMAM-Au displacement induced by acoustic streaming. The result also demonstrated that the specificity and selectivity of the sensing platform under acoustic actuation are comparable to the static incubation in detecting the targeted antibody. Institute of Physics 2024 Article PeerReviewed Syamila, Noor and Hamzah, Amir Syahir Amir and Laurell, Thomas and Sulaiman, Yusran and Ikeno, Shinya and Siang Tan, Wen and Ahmad Tajudin, Asilah (2024) Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform. Journal of Micromechanics and Microengineering, 34 (1). ISSN 0960-1317; ESSN: 1361-6439 https://iopscience.iop.org/article/10.1088/1361-6439/ad0e42 10.1088/1361-6439/ad0e42 |
| spellingShingle | Syamila, Noor Hamzah, Amir Syahir Amir Laurell, Thomas Sulaiman, Yusran Ikeno, Shinya Siang Tan, Wen Ahmad Tajudin, Asilah Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform |
| title | Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform |
| title_full | Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform |
| title_fullStr | Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform |
| title_full_unstemmed | Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform |
| title_short | Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform |
| title_sort | displacement of pamam au via acoustic streaming on an electrochemical immunosensing platform |
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