Manganese and copper-coated nickel oxide nanoparticles synthesized from Carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53
In the present work, manganese–copper co-infused nickel oxide nanoparticles (MnCu co-doped NiO NPs) were formulated via a green process using Carica papaya extract. The MnCu co-doped NiO NPs were characterized by X-ray diffraction (XRD), UV–Vis, Fourier transform infrared, field emission scanning el...
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| Format: | Article |
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Walter de Gruyter GmbH
2024
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| _version_ | 1811137741446971392 |
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| author | Alzahrani, Badr Elderdery, Abozer Y. Alsrhani, Abdullah Alzerwi, Nasser A.N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M.E. Alabdulsalam, Abdulrahim A. Alsultan, Afnan Bakhsh, Ebtisam ALSuhaymi, Naif Kumar, Suresh S. Mok, Pooi Ling |
| author_facet | Alzahrani, Badr Elderdery, Abozer Y. Alsrhani, Abdullah Alzerwi, Nasser A.N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M.E. Alabdulsalam, Abdulrahim A. Alsultan, Afnan Bakhsh, Ebtisam ALSuhaymi, Naif Kumar, Suresh S. Mok, Pooi Ling |
| author_sort | Alzahrani, Badr |
| collection | UPM |
| description | In the present work, manganese–copper co-infused nickel oxide nanoparticles (MnCu co-doped NiO NPs) were formulated via a green process using Carica papaya extract. The MnCu co-doped NiO NPs were characterized by X-ray diffraction (XRD), UV–Vis, Fourier transform infrared, field emission scanning electron microscope, energy dispersive X-ray analysis, and photoluminescence (PL) spectrum. The XRD pattern demonstrated that synthesized MnCu codoped NiO NPs exhibit cubic structure. On the PL spectrum, various surface defects were identified. MnCu co-doped NiO NPs exhibited ferromagnetic properties at 37°C. The antimicrobial activity of green synthesis MnCu co-doped NiO NPs against human pathogens (Escherichia coli, Streptococcus pneumoniae, Bacillus megaterium, Bacillus subtilis, Shigella dysenteriae, Pseudomonas aeruginosa) and Candida albicans as fungal strains were demonstrated. The MnCu co-doped NiO NPs treatment considerably reduced MDA-MB-231 cell viability while not disturbing HBL-100 cell viability. Different fluorescent staining analyses revealed that MnCu co-doped NiO NPs induced nuclear and mitochondrial damage to improve free radical production, altering mitochondrial membrane protein potential, which led to apoptotic cell death in MDA-MB-231 cells. The MnCu co-doped NiO NP treatment enhanced pro-apoptotic protein expression and inhibited the cell cycle at the S phase in MDA-MB-231 cells. This makes it easy, cheap, and environmentally friendly to make MnCu co-doped NiO NPs using C. papaya extract, which has excellent antimicrobial properties. |
| first_indexed | 2024-09-25T03:39:07Z |
| format | Article |
| id | upm.eprints-106233 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:39:07Z |
| publishDate | 2024 |
| publisher | Walter de Gruyter GmbH |
| record_format | dspace |
| spelling | upm.eprints-1062332024-05-08T13:48:40Z http://psasir.upm.edu.my/id/eprint/106233/ Manganese and copper-coated nickel oxide nanoparticles synthesized from Carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53 Alzahrani, Badr Elderdery, Abozer Y. Alsrhani, Abdullah Alzerwi, Nasser A.N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M.E. Alabdulsalam, Abdulrahim A. Alsultan, Afnan Bakhsh, Ebtisam ALSuhaymi, Naif Kumar, Suresh S. Mok, Pooi Ling In the present work, manganese–copper co-infused nickel oxide nanoparticles (MnCu co-doped NiO NPs) were formulated via a green process using Carica papaya extract. The MnCu co-doped NiO NPs were characterized by X-ray diffraction (XRD), UV–Vis, Fourier transform infrared, field emission scanning electron microscope, energy dispersive X-ray analysis, and photoluminescence (PL) spectrum. The XRD pattern demonstrated that synthesized MnCu codoped NiO NPs exhibit cubic structure. On the PL spectrum, various surface defects were identified. MnCu co-doped NiO NPs exhibited ferromagnetic properties at 37°C. The antimicrobial activity of green synthesis MnCu co-doped NiO NPs against human pathogens (Escherichia coli, Streptococcus pneumoniae, Bacillus megaterium, Bacillus subtilis, Shigella dysenteriae, Pseudomonas aeruginosa) and Candida albicans as fungal strains were demonstrated. The MnCu co-doped NiO NPs treatment considerably reduced MDA-MB-231 cell viability while not disturbing HBL-100 cell viability. Different fluorescent staining analyses revealed that MnCu co-doped NiO NPs induced nuclear and mitochondrial damage to improve free radical production, altering mitochondrial membrane protein potential, which led to apoptotic cell death in MDA-MB-231 cells. The MnCu co-doped NiO NP treatment enhanced pro-apoptotic protein expression and inhibited the cell cycle at the S phase in MDA-MB-231 cells. This makes it easy, cheap, and environmentally friendly to make MnCu co-doped NiO NPs using C. papaya extract, which has excellent antimicrobial properties. Walter de Gruyter GmbH 2024 Article PeerReviewed Alzahrani, Badr and Elderdery, Abozer Y. and Alsrhani, Abdullah and Alzerwi, Nasser A.N. and Althobiti, Maryam Musleh and Rayzah, Musaed and Idrees, Bandar and Elkhalifa, Ahmed M.E. and Alabdulsalam, Abdulrahim A. and Alsultan, Afnan and Bakhsh, Ebtisam and ALSuhaymi, Naif and Kumar, Suresh S. and Mok, Pooi Ling (2024) Manganese and copper-coated nickel oxide nanoparticles synthesized from Carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53. Green Processing and Synthesis, 13 (1). pp. 1-17. ISSN 2191-9542; ESSN: 2191-9550 https://www.degruyter.com/document/doi/10.1515/gps-2023-0087/html 10.1515/gps-2023-0087 |
| spellingShingle | Alzahrani, Badr Elderdery, Abozer Y. Alsrhani, Abdullah Alzerwi, Nasser A.N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M.E. Alabdulsalam, Abdulrahim A. Alsultan, Afnan Bakhsh, Ebtisam ALSuhaymi, Naif Kumar, Suresh S. Mok, Pooi Ling Manganese and copper-coated nickel oxide nanoparticles synthesized from Carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53 |
| title | Manganese and copper-coated nickel oxide nanoparticles synthesized from Carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53 |
| title_full | Manganese and copper-coated nickel oxide nanoparticles synthesized from Carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53 |
| title_fullStr | Manganese and copper-coated nickel oxide nanoparticles synthesized from Carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53 |
| title_full_unstemmed | Manganese and copper-coated nickel oxide nanoparticles synthesized from Carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53 |
| title_short | Manganese and copper-coated nickel oxide nanoparticles synthesized from Carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53 |
| title_sort | manganese and copper coated nickel oxide nanoparticles synthesized from carica papaya leaf extract induce antimicrobial activity and breast cancer cell death by triggering mitochondrial caspases and p53 |
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