Biodegradation of monocrotophos by Brucella intermedia Msd2 isolated from cotton plant
Widespread and inadequate use of Monocrotophos has led to several environmental issues. Biodegradation is an ecofriendly method used for detoxifcation of toxic monocrotophos. In the present study, Msd2 bacterial strain was isolated from the cotton plant growing in contaminated sites of Sahiwal, Paki...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Springer Dordrecht
2023
|
| _version_ | 1817927415775625216 |
|---|---|
| author | Shazmin, undefined Ahmad, Siti Aqlima Naqvi, Tatheer Alam Munis, Muhammad Farooq Hussain Javed, Muhammad Tariq Chaudhary, Hassan Javed |
| author_facet | Shazmin, undefined Ahmad, Siti Aqlima Naqvi, Tatheer Alam Munis, Muhammad Farooq Hussain Javed, Muhammad Tariq Chaudhary, Hassan Javed |
| author_sort | Shazmin, undefined |
| collection | UPM |
| description | Widespread and inadequate use of Monocrotophos has led to several environmental issues. Biodegradation is an ecofriendly method used for detoxifcation of toxic monocrotophos. In the present study, Msd2 bacterial strain was isolated from the cotton plant growing in contaminated sites of Sahiwal, Pakistan. Msd2 is capable of utilizing the monocrotophos (MCP) organophosphate pesticide as its sole carbon source for growth. Msd2 was identifed as Brucella intermedia on the basis of morphology, biochemical characterization and 16S rRNA sequencing. B. intermedia showed tolerance of MCP up to 100 ppm. The presence of opd candidate gene for pesticide degradation, gives credence to B. intermedia as an efective bacterium to degrade MCP. Screening of the B. intermedia strain Msd2 for plant growth promoting activities revealed its ability to produce ammonia, exopolysaccharides, catalase, amylase and ACC-deaminase, and phosphorus, zinc and potassium solubilization. The optimization of the growth parameters (temperatures, shaking rpm, and pH level) of the MCP-degrading isolate was carried out in minimal salt broth supplemented with MCP. The optimal pH, temperature, and rpm for Msd2 growth were observed as pH 6, 35 °C, and 120 rpm, respectively. Based on optimization results, batch degradation experiment was performed. Biodegradation of MCP by B. intermedia was monitored using HPLC and recorded 78 degradation of MCP at 100 ppm concentration within 7 days of incubation. Degradation of MCP by Msd2 followed the frst order reaction kinetics. Plant growth promoting and multi-stress tolerance ability of Msd2 was confrmed by molecular analysis. It is concluded that Brucella intermedia strain Msd2 could be benefcial as potential biological agent for an efective bioremediation for polluted environments. |
| first_indexed | 2024-12-09T02:18:05Z |
| format | Article |
| id | upm.eprints-106882 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-12-09T02:18:05Z |
| publishDate | 2023 |
| publisher | Springer Dordrecht |
| record_format | dspace |
| spelling | upm.eprints-1068822024-10-03T04:27:52Z http://psasir.upm.edu.my/id/eprint/106882/ Biodegradation of monocrotophos by Brucella intermedia Msd2 isolated from cotton plant Shazmin, undefined Ahmad, Siti Aqlima Naqvi, Tatheer Alam Munis, Muhammad Farooq Hussain Javed, Muhammad Tariq Chaudhary, Hassan Javed Widespread and inadequate use of Monocrotophos has led to several environmental issues. Biodegradation is an ecofriendly method used for detoxifcation of toxic monocrotophos. In the present study, Msd2 bacterial strain was isolated from the cotton plant growing in contaminated sites of Sahiwal, Pakistan. Msd2 is capable of utilizing the monocrotophos (MCP) organophosphate pesticide as its sole carbon source for growth. Msd2 was identifed as Brucella intermedia on the basis of morphology, biochemical characterization and 16S rRNA sequencing. B. intermedia showed tolerance of MCP up to 100 ppm. The presence of opd candidate gene for pesticide degradation, gives credence to B. intermedia as an efective bacterium to degrade MCP. Screening of the B. intermedia strain Msd2 for plant growth promoting activities revealed its ability to produce ammonia, exopolysaccharides, catalase, amylase and ACC-deaminase, and phosphorus, zinc and potassium solubilization. The optimization of the growth parameters (temperatures, shaking rpm, and pH level) of the MCP-degrading isolate was carried out in minimal salt broth supplemented with MCP. The optimal pH, temperature, and rpm for Msd2 growth were observed as pH 6, 35 °C, and 120 rpm, respectively. Based on optimization results, batch degradation experiment was performed. Biodegradation of MCP by B. intermedia was monitored using HPLC and recorded 78 degradation of MCP at 100 ppm concentration within 7 days of incubation. Degradation of MCP by Msd2 followed the frst order reaction kinetics. Plant growth promoting and multi-stress tolerance ability of Msd2 was confrmed by molecular analysis. It is concluded that Brucella intermedia strain Msd2 could be benefcial as potential biological agent for an efective bioremediation for polluted environments. Springer Dordrecht 2023-03-31 Article PeerReviewed Shazmin, undefined and Ahmad, Siti Aqlima and Naqvi, Tatheer Alam and Munis, Muhammad Farooq Hussain and Javed, Muhammad Tariq and Chaudhary, Hassan Javed (2023) Biodegradation of monocrotophos by Brucella intermedia Msd2 isolated from cotton plant. World Journal of Microbiology & Biotechnology, 39 (6). art. no. 141. pp. 1-13. ISSN 0959-3993; ESSN: 1573-0972 https://link.springer.com/article/10.1007/s11274-023-03575-7 10.1007/s11274-023-03575-7 |
| spellingShingle | Shazmin, undefined Ahmad, Siti Aqlima Naqvi, Tatheer Alam Munis, Muhammad Farooq Hussain Javed, Muhammad Tariq Chaudhary, Hassan Javed Biodegradation of monocrotophos by Brucella intermedia Msd2 isolated from cotton plant |
| title | Biodegradation of monocrotophos by Brucella intermedia Msd2 isolated from cotton plant |
| title_full | Biodegradation of monocrotophos by Brucella intermedia Msd2 isolated from cotton plant |
| title_fullStr | Biodegradation of monocrotophos by Brucella intermedia Msd2 isolated from cotton plant |
| title_full_unstemmed | Biodegradation of monocrotophos by Brucella intermedia Msd2 isolated from cotton plant |
| title_short | Biodegradation of monocrotophos by Brucella intermedia Msd2 isolated from cotton plant |
| title_sort | biodegradation of monocrotophos by brucella intermedia msd2 isolated from cotton plant |
| work_keys_str_mv | AT shazminundefined biodegradationofmonocrotophosbybrucellaintermediamsd2isolatedfromcottonplant AT ahmadsitiaqlima biodegradationofmonocrotophosbybrucellaintermediamsd2isolatedfromcottonplant AT naqvitatheeralam biodegradationofmonocrotophosbybrucellaintermediamsd2isolatedfromcottonplant AT munismuhammadfarooqhussain biodegradationofmonocrotophosbybrucellaintermediamsd2isolatedfromcottonplant AT javedmuhammadtariq biodegradationofmonocrotophosbybrucellaintermediamsd2isolatedfromcottonplant AT chaudharyhassanjaved biodegradationofmonocrotophosbybrucellaintermediamsd2isolatedfromcottonplant |