Biosynthesis of phyto-functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties
The green synthesis of nanomaterials is of utmost interest as it offers an eco-friendly approach over chemical synthetic routes. However, the reported biosynthesis methods are often time-consuming and require heating or mechanical stirring. The current study reports a facile one-pot biosynthesis of...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Frontiers Media SA
2023
|
| _version_ | 1825939423623118848 |
|---|---|
| author | Ullah, Sami Khalid, Rimsha Rehman, Muhammad F. Irfan, Muhammad I. Abbas, Azhar Alhoshani, Ali Anwar, Farooq Amin, Hatem M. A. |
| author_facet | Ullah, Sami Khalid, Rimsha Rehman, Muhammad F. Irfan, Muhammad I. Abbas, Azhar Alhoshani, Ali Anwar, Farooq Amin, Hatem M. A. |
| author_sort | Ullah, Sami |
| collection | UPM |
| description | The green synthesis of nanomaterials is of utmost interest as it offers an eco-friendly approach over chemical synthetic routes. However, the reported biosynthesis methods are often time-consuming and require heating or mechanical stirring. The current study reports a facile one-pot biosynthesis of silver nanoparticles (AgNPs) mediated by olive fruit extract (OFE) and sunlight irradiation of only 20 s. OFE acts as both a reducing and a capping agent for the formation of OFE-capped AgNPs (AgNPs@OFE). The as-synthesized NPs were systematically characterized by UV-vis spectrometry, Fourier transform infrared (FTIR) spectroscopy, scanning electrochemical microscopy with energy-dispersive X-ray (SEM-EDX), X-ray diffraction (XRD), dynamic light scattering (DLS), and cyclic voltammetry. SEM images confirmed the successful formation of monodispersed spherical AgNPs@OFE of approximately 77 nm. FTIR spectroscopy suggested the involvement of functional groups of phytochemicals from the OFE in the capping and reduction of Ag+ to Ag. The particles revealed excellent colloidal stability as evidenced from the high zeta potential (ZP) value (ˆ’40 mV). Interestingly, using the disk diffusion method, AgNPs@OFE revealed higher inhibition efficiency against Gram-negative bacteria (Escherichia coli, Klebsiella oxytoca, and extensively drug-resistant (XDR) Salmonella typhi) than Gram-positive bacteria (Staphylococcus aureus), with Escherichia coli showing the highest inhibition zone of 27 mm. In addition, AgNPs@OFE exhibited maximum potent antioxidant scavenging potential against H2O2, followed by DPPH, O2ˆ’, and OHˆ’ free radicals. Overall, OFE can be considered an effective source for the sustainable production of stable AgNPs with potential antioxidant and antibacterial activities for biomedical applications. |
| first_indexed | 2024-09-25T03:39:52Z |
| format | Article |
| id | upm.eprints-106900 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:39:52Z |
| publishDate | 2023 |
| publisher | Frontiers Media SA |
| record_format | dspace |
| spelling | upm.eprints-1069002024-08-05T08:42:04Z http://psasir.upm.edu.my/id/eprint/106900/ Biosynthesis of phyto-functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties Ullah, Sami Khalid, Rimsha Rehman, Muhammad F. Irfan, Muhammad I. Abbas, Azhar Alhoshani, Ali Anwar, Farooq Amin, Hatem M. A. The green synthesis of nanomaterials is of utmost interest as it offers an eco-friendly approach over chemical synthetic routes. However, the reported biosynthesis methods are often time-consuming and require heating or mechanical stirring. The current study reports a facile one-pot biosynthesis of silver nanoparticles (AgNPs) mediated by olive fruit extract (OFE) and sunlight irradiation of only 20 s. OFE acts as both a reducing and a capping agent for the formation of OFE-capped AgNPs (AgNPs@OFE). The as-synthesized NPs were systematically characterized by UV-vis spectrometry, Fourier transform infrared (FTIR) spectroscopy, scanning electrochemical microscopy with energy-dispersive X-ray (SEM-EDX), X-ray diffraction (XRD), dynamic light scattering (DLS), and cyclic voltammetry. SEM images confirmed the successful formation of monodispersed spherical AgNPs@OFE of approximately 77 nm. FTIR spectroscopy suggested the involvement of functional groups of phytochemicals from the OFE in the capping and reduction of Ag+ to Ag. The particles revealed excellent colloidal stability as evidenced from the high zeta potential (ZP) value (ˆ’40 mV). Interestingly, using the disk diffusion method, AgNPs@OFE revealed higher inhibition efficiency against Gram-negative bacteria (Escherichia coli, Klebsiella oxytoca, and extensively drug-resistant (XDR) Salmonella typhi) than Gram-positive bacteria (Staphylococcus aureus), with Escherichia coli showing the highest inhibition zone of 27 mm. In addition, AgNPs@OFE exhibited maximum potent antioxidant scavenging potential against H2O2, followed by DPPH, O2ˆ’, and OHˆ’ free radicals. Overall, OFE can be considered an effective source for the sustainable production of stable AgNPs with potential antioxidant and antibacterial activities for biomedical applications. Frontiers Media SA 2023 Article PeerReviewed Ullah, Sami and Khalid, Rimsha and Rehman, Muhammad F. and Irfan, Muhammad I. and Abbas, Azhar and Alhoshani, Ali and Anwar, Farooq and Amin, Hatem M. A. (2023) Biosynthesis of phyto-functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties. Frontiers in Chemistry, 11. pp. 1-14. ISSN 2296-2646 https://www.frontiersin.org/articles/10.3389/fchem.2023.1202252/full 10.3389/fchem.2023.1202252 |
| spellingShingle | Ullah, Sami Khalid, Rimsha Rehman, Muhammad F. Irfan, Muhammad I. Abbas, Azhar Alhoshani, Ali Anwar, Farooq Amin, Hatem M. A. Biosynthesis of phyto-functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties |
| title | Biosynthesis of phyto-functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties |
| title_full | Biosynthesis of phyto-functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties |
| title_fullStr | Biosynthesis of phyto-functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties |
| title_full_unstemmed | Biosynthesis of phyto-functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties |
| title_short | Biosynthesis of phyto-functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties |
| title_sort | biosynthesis of phyto functionalized silver nanoparticles using olive fruit extract and evaluation of their antibacterial and antioxidant properties |
| work_keys_str_mv | AT ullahsami biosynthesisofphytofunctionalizedsilvernanoparticlesusingolivefruitextractandevaluationoftheirantibacterialandantioxidantproperties AT khalidrimsha biosynthesisofphytofunctionalizedsilvernanoparticlesusingolivefruitextractandevaluationoftheirantibacterialandantioxidantproperties AT rehmanmuhammadf biosynthesisofphytofunctionalizedsilvernanoparticlesusingolivefruitextractandevaluationoftheirantibacterialandantioxidantproperties AT irfanmuhammadi biosynthesisofphytofunctionalizedsilvernanoparticlesusingolivefruitextractandevaluationoftheirantibacterialandantioxidantproperties AT abbasazhar biosynthesisofphytofunctionalizedsilvernanoparticlesusingolivefruitextractandevaluationoftheirantibacterialandantioxidantproperties AT alhoshaniali biosynthesisofphytofunctionalizedsilvernanoparticlesusingolivefruitextractandevaluationoftheirantibacterialandantioxidantproperties AT anwarfarooq biosynthesisofphytofunctionalizedsilvernanoparticlesusingolivefruitextractandevaluationoftheirantibacterialandantioxidantproperties AT aminhatemma biosynthesisofphytofunctionalizedsilvernanoparticlesusingolivefruitextractandevaluationoftheirantibacterialandantioxidantproperties |