Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation
The present study determined the in-situ antibacterial effect of cationic kenaf seed peptides mixture (KSPM) in whole milk and further comprehensively elucidated the mechanisms underlying the bactericidal action against gram-positive and gram-negative bacteria. The application experiments in artific...
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| Format: | Article |
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Elsevier
2023
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| _version_ | 1811137806951514112 |
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| author | Arulrajah, Brisha Qoms, Mohammed S. Muhialdin, Belal J. Meor Hussin, Anis Shobirin Hasan, Hanan Zarei, Mohammad Chau, De-Ming Ramasamy, Rajesh Saari, Nazamid |
| author_facet | Arulrajah, Brisha Qoms, Mohammed S. Muhialdin, Belal J. Meor Hussin, Anis Shobirin Hasan, Hanan Zarei, Mohammad Chau, De-Ming Ramasamy, Rajesh Saari, Nazamid |
| author_sort | Arulrajah, Brisha |
| collection | UPM |
| description | The present study determined the in-situ antibacterial effect of cationic kenaf seed peptides mixture (KSPM) in whole milk and further comprehensively elucidated the mechanisms underlying the bactericidal action against gram-positive and gram-negative bacteria. The application experiments in artificially contaminated milk showed that KSPM at 0.5 and 1 mg/mL displayed a stable and strong bactericidal activity within 1“6 h of treatment and lasted up to two weeks storage at room and refrigeration conditions. The in-vitro mechanistic study showed that the KSPM underwent conformational changes from random coil to ±-helix and ²-sheet in the membrane-mimetic environment. The KSPM exerted its inhibitory effect in a concentration and time dependent manner through cell membrane permeabilisation associated with membrane-disrupting effects, which is characterised by the cellular material leakage. The in-silico study revealed that the peptides efficiently interacted with bacterial peptidoglycan via hydrogen bonding, electrostatic interactions and hydrophobic forces. The peptide RALPSEHNK interacted with gram-positive and gram-negative bacterial phospholipid phosphate head group mainly through electrostatic interaction. The current work indicated that KSPM exerts its bactericidal effect by membrane-targeted mechanism and it would be a promising natural candidate to control bacterial contamination in milk and dairy-based products. |
| first_indexed | 2024-09-25T03:40:09Z |
| format | Article |
| id | upm.eprints-107651 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:40:09Z |
| publishDate | 2023 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-1076512024-09-12T04:06:21Z http://psasir.upm.edu.my/id/eprint/107651/ Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation Arulrajah, Brisha Qoms, Mohammed S. Muhialdin, Belal J. Meor Hussin, Anis Shobirin Hasan, Hanan Zarei, Mohammad Chau, De-Ming Ramasamy, Rajesh Saari, Nazamid The present study determined the in-situ antibacterial effect of cationic kenaf seed peptides mixture (KSPM) in whole milk and further comprehensively elucidated the mechanisms underlying the bactericidal action against gram-positive and gram-negative bacteria. The application experiments in artificially contaminated milk showed that KSPM at 0.5 and 1 mg/mL displayed a stable and strong bactericidal activity within 1“6 h of treatment and lasted up to two weeks storage at room and refrigeration conditions. The in-vitro mechanistic study showed that the KSPM underwent conformational changes from random coil to ±-helix and ²-sheet in the membrane-mimetic environment. The KSPM exerted its inhibitory effect in a concentration and time dependent manner through cell membrane permeabilisation associated with membrane-disrupting effects, which is characterised by the cellular material leakage. The in-silico study revealed that the peptides efficiently interacted with bacterial peptidoglycan via hydrogen bonding, electrostatic interactions and hydrophobic forces. The peptide RALPSEHNK interacted with gram-positive and gram-negative bacterial phospholipid phosphate head group mainly through electrostatic interaction. The current work indicated that KSPM exerts its bactericidal effect by membrane-targeted mechanism and it would be a promising natural candidate to control bacterial contamination in milk and dairy-based products. Elsevier 2023-05 Article PeerReviewed Arulrajah, Brisha and Qoms, Mohammed S. and Muhialdin, Belal J. and Meor Hussin, Anis Shobirin and Hasan, Hanan and Zarei, Mohammad and Chau, De-Ming and Ramasamy, Rajesh and Saari, Nazamid (2023) Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation. LWT - Food Science and Technology, 181. art. no. 114757. ISSN 0023-6438 https://linkinghub.elsevier.com/retrieve/pii/S0023643823003365 10.1016/j.lwt.2023.114757 |
| spellingShingle | Arulrajah, Brisha Qoms, Mohammed S. Muhialdin, Belal J. Meor Hussin, Anis Shobirin Hasan, Hanan Zarei, Mohammad Chau, De-Ming Ramasamy, Rajesh Saari, Nazamid Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation |
| title | Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation |
| title_full | Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation |
| title_fullStr | Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation |
| title_full_unstemmed | Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation |
| title_short | Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation |
| title_sort | elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram positive and gram negative bacteria and its efficacy in whole milk preservation |
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