ENPP4 and HOXA3 as potential leukaemia stem cell markers in acute myeloid leukaemia
Introduction: Acute myeloid leukaemia (AML) is a heterogeneous malignant disease with a high degree of treatment failure using chemotherapy. Leukaemia stem cells (LSCs) are CD34+CD38- early progenitors associated with poor prognosis in AML. A unique LSC phenotype that excludes rare normal haematopoi...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Malaysian Society of Pathologists
2023
|
| _version_ | 1825939507287949312 |
|---|---|
| author | Mohd Amin, A Panneerselvan, N Md Noor, S Mohtaruddin, N Sathar, J Norbaya, WS Osman, R Kee, LH Mohd Yaaku, WH Cheong, SK Abdullah, M |
| author_facet | Mohd Amin, A Panneerselvan, N Md Noor, S Mohtaruddin, N Sathar, J Norbaya, WS Osman, R Kee, LH Mohd Yaaku, WH Cheong, SK Abdullah, M |
| author_sort | Mohd Amin, A |
| collection | UPM |
| description | Introduction: Acute myeloid leukaemia (AML) is a heterogeneous malignant disease with a high degree of treatment failure using chemotherapy. Leukaemia stem cells (LSCs) are CD34+CD38- early progenitors associated with poor prognosis in AML. A unique LSC phenotype that excludes rare normal haematopoietic stem cells (HSC) is still elusive. This study aimed to determine expression of selected potential LSC markers in normal and leukaemic myeloid cells and correlate prognosis in AML patients.
Materials and methods: Flow cytometry and RT-qPCR measured expressions of ALDH, IL3RA/CD123, CLEC12A/CLL-1/CD371, HOXA3 and ENPP4. Normal cord blood (n=3) and blood monocytes (n=5) represented HSC and mature cells, respectively. Myeloid leukaemia cell lines (THP-1, KG-1a, K562 and HL-60) represented progenitor cells at various stages of maturation. AML samples included chemo-resistant (n=8), early relapse (n=2) and late relapse (n=18).
Results: Combining protein/gene expressions, CD34+CD38- was a feature of immature cells seen in cord blood, KG-1a, and K562 but not more mature cells (blood monocytes and HL-60). Normal cells expressed CD371 while mature cells (blood monocytes and HL-60) lacked CD123. ENPP4 was not expressed on normal cells while HOXA3 was expressed only on cord blood and THP-1. In AML, CD123, HOXA3, ENPP4 (but not CD371) were significantly increased in the CD34+CD38- fraction of chemo-resistant patients while ALDH was associated with chemo-resistance.
Conclusion: CD34+CD38- presented an immature phenotype and with ALDH were associated with poor prognosis. CD123, HOXA3 and ENPP4 further enriched the LSC population. ENPP4 has not been reported and has the advantage of not being expressed on HSC and normal monocytes. |
| first_indexed | 2024-12-09T02:19:30Z |
| format | Article |
| id | upm.eprints-107715 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-12-09T02:19:30Z |
| publishDate | 2023 |
| publisher | Malaysian Society of Pathologists |
| record_format | dspace |
| spelling | upm.eprints-1077152024-10-28T02:45:35Z http://psasir.upm.edu.my/id/eprint/107715/ ENPP4 and HOXA3 as potential leukaemia stem cell markers in acute myeloid leukaemia Mohd Amin, A Panneerselvan, N Md Noor, S Mohtaruddin, N Sathar, J Norbaya, WS Osman, R Kee, LH Mohd Yaaku, WH Cheong, SK Abdullah, M Introduction: Acute myeloid leukaemia (AML) is a heterogeneous malignant disease with a high degree of treatment failure using chemotherapy. Leukaemia stem cells (LSCs) are CD34+CD38- early progenitors associated with poor prognosis in AML. A unique LSC phenotype that excludes rare normal haematopoietic stem cells (HSC) is still elusive. This study aimed to determine expression of selected potential LSC markers in normal and leukaemic myeloid cells and correlate prognosis in AML patients. Materials and methods: Flow cytometry and RT-qPCR measured expressions of ALDH, IL3RA/CD123, CLEC12A/CLL-1/CD371, HOXA3 and ENPP4. Normal cord blood (n=3) and blood monocytes (n=5) represented HSC and mature cells, respectively. Myeloid leukaemia cell lines (THP-1, KG-1a, K562 and HL-60) represented progenitor cells at various stages of maturation. AML samples included chemo-resistant (n=8), early relapse (n=2) and late relapse (n=18). Results: Combining protein/gene expressions, CD34+CD38- was a feature of immature cells seen in cord blood, KG-1a, and K562 but not more mature cells (blood monocytes and HL-60). Normal cells expressed CD371 while mature cells (blood monocytes and HL-60) lacked CD123. ENPP4 was not expressed on normal cells while HOXA3 was expressed only on cord blood and THP-1. In AML, CD123, HOXA3, ENPP4 (but not CD371) were significantly increased in the CD34+CD38- fraction of chemo-resistant patients while ALDH was associated with chemo-resistance. Conclusion: CD34+CD38- presented an immature phenotype and with ALDH were associated with poor prognosis. CD123, HOXA3 and ENPP4 further enriched the LSC population. ENPP4 has not been reported and has the advantage of not being expressed on HSC and normal monocytes. Malaysian Society of Pathologists 2023 Article PeerReviewed Mohd Amin, A and Panneerselvan, N and Md Noor, S and Mohtaruddin, N and Sathar, J and Norbaya, WS and Osman, R and Kee, LH and Mohd Yaaku, WH and Cheong, SK and Abdullah, M (2023) ENPP4 and HOXA3 as potential leukaemia stem cell markers in acute myeloid leukaemia. Malaysian Journal of Pathology, 45 (1). pp. 65-76. ISSN 0126-8635 https://openurl.ebsco.com/EPDB%3Agcd%3A15%3A29989151/detailv2?sid=ebsco%3Aocu%3Arecord&id=ebsco%3Agcd%3A163418079&bquery=IS%200126-8635%20AND%20VI%2045%20AND%20IP%201%20AND%20DT%202023&page=1 |
| spellingShingle | Mohd Amin, A Panneerselvan, N Md Noor, S Mohtaruddin, N Sathar, J Norbaya, WS Osman, R Kee, LH Mohd Yaaku, WH Cheong, SK Abdullah, M ENPP4 and HOXA3 as potential leukaemia stem cell markers in acute myeloid leukaemia |
| title | ENPP4 and HOXA3 as potential leukaemia stem cell markers in acute myeloid leukaemia |
| title_full | ENPP4 and HOXA3 as potential leukaemia stem cell markers in acute myeloid leukaemia |
| title_fullStr | ENPP4 and HOXA3 as potential leukaemia stem cell markers in acute myeloid leukaemia |
| title_full_unstemmed | ENPP4 and HOXA3 as potential leukaemia stem cell markers in acute myeloid leukaemia |
| title_short | ENPP4 and HOXA3 as potential leukaemia stem cell markers in acute myeloid leukaemia |
| title_sort | enpp4 and hoxa3 as potential leukaemia stem cell markers in acute myeloid leukaemia |
| work_keys_str_mv | AT mohdamina enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT panneerselvann enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT mdnoors enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT mohtaruddinn enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT satharj enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT norbayaws enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT osmanr enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT keelh enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT mohdyaakuwh enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT cheongsk enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia AT abdullahm enpp4andhoxa3aspotentialleukaemiastemcellmarkersinacutemyeloidleukaemia |