Ethanolic extract of Polygonum minus protects differentiated human neuroblastoma Cells (SH-SY5Y) against H2O2-Induced oxidative stress
Neuronal models are an important tool in neuroscientific research. Hydrogen peroxide (H2O2), a major risk factor of neuronal oxidative stress, initiates a cascade of neuronal cell death. Polygonum minus Huds, known as ‘kesum’, is widely used in traditional medicine. P. minus has been reported to exh...
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2023
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| _version_ | 1817927513809092608 |
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| author | Sayuti, Nor Hafiza Zulkefli, Nabilah Tan, Jen Kit Saad, Norazalina Baharum, Syarul Nataqain Hamezah, Hamizah Shahirah Bunawan, Hamidun Ahmed, Qamar Uddin Parveen, Humaira Mukhtar, Sayeed Alsharif, Meshari A. Sarian, Murni Nazira |
| author_facet | Sayuti, Nor Hafiza Zulkefli, Nabilah Tan, Jen Kit Saad, Norazalina Baharum, Syarul Nataqain Hamezah, Hamizah Shahirah Bunawan, Hamidun Ahmed, Qamar Uddin Parveen, Humaira Mukhtar, Sayeed Alsharif, Meshari A. Sarian, Murni Nazira |
| author_sort | Sayuti, Nor Hafiza |
| collection | UPM |
| description | Neuronal models are an important tool in neuroscientific research. Hydrogen peroxide (H2O2), a major risk factor of neuronal oxidative stress, initiates a cascade of neuronal cell death. Polygonum minus Huds, known as ‘kesum’, is widely used in traditional medicine. P. minus has been reported to exhibit a few medicinal and pharmacological properties. The current study aimed to investigate the neuroprotective effects of P. minus ethanolic extract (PMEE) on H2O2-induced neurotoxicity in SH-SY5Y cells. LC–MS/MS revealed the presence of 28 metabolites in PMEE. Our study showed that the PMEE provided neuroprotection against H2O2-induced oxidative stress by activating the Nrf2/ARE, NF-κB/IκB and MAPK signaling pathways in PMEE pre-treated differentiated SH-SY5Y cells. Meanwhile, the acetylcholine (ACH) level was increased in the oxidative stress-induced treatment group after 4 h of exposure with H2O2. Molecular docking results with acetylcholinesterase (AChE) depicted that quercitrin showed the highest docking score at −9.5 kcal/mol followed by aloe-emodin, afzelin, and citreorosein at −9.4, −9.3 and −9.0 kcal/mol, respectively, compared to the other PMEE’s identified compounds, which show lower docking scores. The results indicate that PMEE has neuroprotective effects on SH-SY5Y neuroblastoma cells in vitro. In conclusion, PMEE may aid in reducing oxidative stress as a preventative therapy for neurodegenerative diseases. |
| first_indexed | 2024-12-09T02:19:38Z |
| format | Article |
| id | upm.eprints-107754 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-12-09T02:19:38Z |
| publishDate | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-1077542024-10-28T03:59:34Z http://psasir.upm.edu.my/id/eprint/107754/ Ethanolic extract of Polygonum minus protects differentiated human neuroblastoma Cells (SH-SY5Y) against H2O2-Induced oxidative stress Sayuti, Nor Hafiza Zulkefli, Nabilah Tan, Jen Kit Saad, Norazalina Baharum, Syarul Nataqain Hamezah, Hamizah Shahirah Bunawan, Hamidun Ahmed, Qamar Uddin Parveen, Humaira Mukhtar, Sayeed Alsharif, Meshari A. Sarian, Murni Nazira Neuronal models are an important tool in neuroscientific research. Hydrogen peroxide (H2O2), a major risk factor of neuronal oxidative stress, initiates a cascade of neuronal cell death. Polygonum minus Huds, known as ‘kesum’, is widely used in traditional medicine. P. minus has been reported to exhibit a few medicinal and pharmacological properties. The current study aimed to investigate the neuroprotective effects of P. minus ethanolic extract (PMEE) on H2O2-induced neurotoxicity in SH-SY5Y cells. LC–MS/MS revealed the presence of 28 metabolites in PMEE. Our study showed that the PMEE provided neuroprotection against H2O2-induced oxidative stress by activating the Nrf2/ARE, NF-κB/IκB and MAPK signaling pathways in PMEE pre-treated differentiated SH-SY5Y cells. Meanwhile, the acetylcholine (ACH) level was increased in the oxidative stress-induced treatment group after 4 h of exposure with H2O2. Molecular docking results with acetylcholinesterase (AChE) depicted that quercitrin showed the highest docking score at −9.5 kcal/mol followed by aloe-emodin, afzelin, and citreorosein at −9.4, −9.3 and −9.0 kcal/mol, respectively, compared to the other PMEE’s identified compounds, which show lower docking scores. The results indicate that PMEE has neuroprotective effects on SH-SY5Y neuroblastoma cells in vitro. In conclusion, PMEE may aid in reducing oxidative stress as a preventative therapy for neurodegenerative diseases. Multidisciplinary Digital Publishing Institute 2023-09-20 Article PeerReviewed Sayuti, Nor Hafiza and Zulkefli, Nabilah and Tan, Jen Kit and Saad, Norazalina and Baharum, Syarul Nataqain and Hamezah, Hamizah Shahirah and Bunawan, Hamidun and Ahmed, Qamar Uddin and Parveen, Humaira and Mukhtar, Sayeed and Alsharif, Meshari A. and Sarian, Murni Nazira (2023) Ethanolic extract of Polygonum minus protects differentiated human neuroblastoma Cells (SH-SY5Y) against H2O2-Induced oxidative stress. Molecules, 28 (18). pp. 6726-6750. ISSN 1420-3049 https://www.mdpi.com/1420-3049/28/18/6726 10.3390/molecules28186726 |
| spellingShingle | Sayuti, Nor Hafiza Zulkefli, Nabilah Tan, Jen Kit Saad, Norazalina Baharum, Syarul Nataqain Hamezah, Hamizah Shahirah Bunawan, Hamidun Ahmed, Qamar Uddin Parveen, Humaira Mukhtar, Sayeed Alsharif, Meshari A. Sarian, Murni Nazira Ethanolic extract of Polygonum minus protects differentiated human neuroblastoma Cells (SH-SY5Y) against H2O2-Induced oxidative stress |
| title | Ethanolic extract of Polygonum minus protects differentiated human neuroblastoma Cells (SH-SY5Y) against H2O2-Induced oxidative stress |
| title_full | Ethanolic extract of Polygonum minus protects differentiated human neuroblastoma Cells (SH-SY5Y) against H2O2-Induced oxidative stress |
| title_fullStr | Ethanolic extract of Polygonum minus protects differentiated human neuroblastoma Cells (SH-SY5Y) against H2O2-Induced oxidative stress |
| title_full_unstemmed | Ethanolic extract of Polygonum minus protects differentiated human neuroblastoma Cells (SH-SY5Y) against H2O2-Induced oxidative stress |
| title_short | Ethanolic extract of Polygonum minus protects differentiated human neuroblastoma Cells (SH-SY5Y) against H2O2-Induced oxidative stress |
| title_sort | ethanolic extract of polygonum minus protects differentiated human neuroblastoma cells sh sy5y against h2o2 induced oxidative stress |
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