Fabrication and characterization of nanodelivery platform based on Chitosan to improve the anticancer outcome of sorafenib in Hepatocellular Carcinoma
Chitosan nanoparticles (CS NPs) showed promising results in drug, vaccine and gene delivery for the treatment of various diseases. The considerable attention towards CS was owning to its outstanding biological properties, however, the main challenge in the application of CS NPs was faced during thei...
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| Format: | Article |
| Language: | English |
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Nature Research
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/107920/1/107920.pdf |
| _version_ | 1825939544404393984 |
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| author | Albalawi, Fahad Hussein, Mohd Zobir Fakurazi, Sharida Masarudin, Mas Jaffri |
| author_facet | Albalawi, Fahad Hussein, Mohd Zobir Fakurazi, Sharida Masarudin, Mas Jaffri |
| author_sort | Albalawi, Fahad |
| collection | UPM |
| description | Chitosan nanoparticles (CS NPs) showed promising results in drug, vaccine and gene delivery for the treatment of various diseases. The considerable attention towards CS was owning to its outstanding biological properties, however, the main challenge in the application of CS NPs was faced during their size-controlled synthesis. Herein, ionic gelation reaction between CS and sodium tripolyphosphate (TPP), a widely used and safe CS cross-linker for biomedical application, was exploited. The development of nanodelivery platform, namely Sorafenib-loaded chitosan nanoparticles (SF–CS NPs), was constructed in order to improve SF drug delivery to human Hepatocellular Carcinoma (HepG2) cell lines. The NPs were artificially fabricated using an ionic gelation technique. A number of CS NPs that had been loaded with an SF were prepared using different concentrations of sodium tripolyphosphate (TPP). These concentrations were 2.5, 5, 10, and 20 mg/mL, and they are abbreviated as SF–CS NPs 2.5, SF–CS NPs 5.0, SF–CS NPs 10, and SF–CS NPs 20 respectively. DLS, FTIR, XRD, HRTEM, TGA, and FESEM with EDX and TEM were used for the physiochemical characterisation of SF–CS NPs. Both DLS and HRTEM techniques demonstrated that smaller particles were produced when the TPP content was raised. In a PBS solution with a pH of 4.5, the SF exhibited efficient release from the nanoparticles, demonstrating that the delivery mechanism is effective for tumour cells. The cytotoxicity investigation showed that their anticancer effect against HepG2 cell lines was significantly superior than that of free SF. In addition, the nanodrug demonstrated an absence of any detectable toxicity to normal adult human dermal fibroblast (HDFa) cell lines. This is a step towards developing a more effective anticancer medication delivery system with sustained-release characteristics, which will ultimately improve the way cancer is managed. |
| first_indexed | 2025-03-07T12:47:26Z |
| format | Article |
| id | upm.eprints-107920 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2025-03-07T12:47:26Z |
| publishDate | 2023 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | upm.eprints-1079202025-03-04T01:17:30Z http://psasir.upm.edu.my/id/eprint/107920/ Fabrication and characterization of nanodelivery platform based on Chitosan to improve the anticancer outcome of sorafenib in Hepatocellular Carcinoma Albalawi, Fahad Hussein, Mohd Zobir Fakurazi, Sharida Masarudin, Mas Jaffri Chitosan nanoparticles (CS NPs) showed promising results in drug, vaccine and gene delivery for the treatment of various diseases. The considerable attention towards CS was owning to its outstanding biological properties, however, the main challenge in the application of CS NPs was faced during their size-controlled synthesis. Herein, ionic gelation reaction between CS and sodium tripolyphosphate (TPP), a widely used and safe CS cross-linker for biomedical application, was exploited. The development of nanodelivery platform, namely Sorafenib-loaded chitosan nanoparticles (SF–CS NPs), was constructed in order to improve SF drug delivery to human Hepatocellular Carcinoma (HepG2) cell lines. The NPs were artificially fabricated using an ionic gelation technique. A number of CS NPs that had been loaded with an SF were prepared using different concentrations of sodium tripolyphosphate (TPP). These concentrations were 2.5, 5, 10, and 20 mg/mL, and they are abbreviated as SF–CS NPs 2.5, SF–CS NPs 5.0, SF–CS NPs 10, and SF–CS NPs 20 respectively. DLS, FTIR, XRD, HRTEM, TGA, and FESEM with EDX and TEM were used for the physiochemical characterisation of SF–CS NPs. Both DLS and HRTEM techniques demonstrated that smaller particles were produced when the TPP content was raised. In a PBS solution with a pH of 4.5, the SF exhibited efficient release from the nanoparticles, demonstrating that the delivery mechanism is effective for tumour cells. The cytotoxicity investigation showed that their anticancer effect against HepG2 cell lines was significantly superior than that of free SF. In addition, the nanodrug demonstrated an absence of any detectable toxicity to normal adult human dermal fibroblast (HDFa) cell lines. This is a step towards developing a more effective anticancer medication delivery system with sustained-release characteristics, which will ultimately improve the way cancer is managed. Nature Research 2023 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/107920/1/107920.pdf Albalawi, Fahad and Hussein, Mohd Zobir and Fakurazi, Sharida and Masarudin, Mas Jaffri (2023) Fabrication and characterization of nanodelivery platform based on Chitosan to improve the anticancer outcome of sorafenib in Hepatocellular Carcinoma. Scientific Reports, 13 (1). art. no. 12180. ISSN 2045-2322; eISSN: 2045-2322 https://www.nature.com/articles/s41598-023-38054-4?error=cookies_not_supported&code=58622012-da25-45ec-a7f8-d466fa1bd36b 10.1038/s41598-023-38054-4 |
| spellingShingle | Albalawi, Fahad Hussein, Mohd Zobir Fakurazi, Sharida Masarudin, Mas Jaffri Fabrication and characterization of nanodelivery platform based on Chitosan to improve the anticancer outcome of sorafenib in Hepatocellular Carcinoma |
| title | Fabrication and characterization of nanodelivery platform based on Chitosan to improve the anticancer outcome of sorafenib in Hepatocellular Carcinoma |
| title_full | Fabrication and characterization of nanodelivery platform based on Chitosan to improve the anticancer outcome of sorafenib in Hepatocellular Carcinoma |
| title_fullStr | Fabrication and characterization of nanodelivery platform based on Chitosan to improve the anticancer outcome of sorafenib in Hepatocellular Carcinoma |
| title_full_unstemmed | Fabrication and characterization of nanodelivery platform based on Chitosan to improve the anticancer outcome of sorafenib in Hepatocellular Carcinoma |
| title_short | Fabrication and characterization of nanodelivery platform based on Chitosan to improve the anticancer outcome of sorafenib in Hepatocellular Carcinoma |
| title_sort | fabrication and characterization of nanodelivery platform based on chitosan to improve the anticancer outcome of sorafenib in hepatocellular carcinoma |
| url | http://psasir.upm.edu.my/id/eprint/107920/1/107920.pdf |
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