HIF-1 sensor in detecting hypoxia tolerance at high altitude
INTRODUCTION: An episode of prolonged exposure to high altitude can cause hypoxia and have significant health consequences. In people with a high-altitude disorder, the body reacts by producing a protein called hypoxia-inducible factor (HIF), which triggers a series of physiological changes and serv...
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| Format: | Article |
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Aerospace Medical Association
2023
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| _version_ | 1825939591018840064 |
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| author | Shaharuddin, Shazreen Nik Abd Rahman, Nik Mohd Afizan Masarudin, Mas Jaffri Alamassi, Mohammedarfat N. Ahmad Saad, Fathinul Fikri |
| author_facet | Shaharuddin, Shazreen Nik Abd Rahman, Nik Mohd Afizan Masarudin, Mas Jaffri Alamassi, Mohammedarfat N. Ahmad Saad, Fathinul Fikri |
| author_sort | Shaharuddin, Shazreen |
| collection | UPM |
| description | INTRODUCTION: An episode of prolonged exposure to high altitude can cause hypoxia and have significant health consequences. In people with a high-altitude disorder, the body reacts by producing a protein called hypoxia-inducible factor (HIF), which triggers a series of physiological changes and serves a central role in the hypoxia response. Its activity is regulated by the oxygen-dependent degradation of the HIF-1α protein (HIF-1A gene). Therefore, the effects of low oxygen tension in high altitude were explored using fluorescent sensors of hypoxia.
METHODS: The development of the sensor provided more sensitivity for detecting hypoxia by generating a calibration of optimized parameters such as reagent concentrations, reagent volumes, and device dimensions.
RESULTS: There is a high sensitivity and specificity in detecting the changes of HIF-1α protein hypoxia using the feasibility hypoxia test. This would enable point-of-care (POC) testing and individual self-administration, resulting in faster and more accurate results that can be used for a robust diagnostic approach and enhanced health surveillance, particularly in high-altitude exposure. |
| first_indexed | 2024-09-25T03:40:37Z |
| format | Article |
| id | upm.eprints-108173 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:40:37Z |
| publishDate | 2023 |
| publisher | Aerospace Medical Association |
| record_format | dspace |
| spelling | upm.eprints-1081732024-09-24T07:18:42Z http://psasir.upm.edu.my/id/eprint/108173/ HIF-1 sensor in detecting hypoxia tolerance at high altitude Shaharuddin, Shazreen Nik Abd Rahman, Nik Mohd Afizan Masarudin, Mas Jaffri Alamassi, Mohammedarfat N. Ahmad Saad, Fathinul Fikri INTRODUCTION: An episode of prolonged exposure to high altitude can cause hypoxia and have significant health consequences. In people with a high-altitude disorder, the body reacts by producing a protein called hypoxia-inducible factor (HIF), which triggers a series of physiological changes and serves a central role in the hypoxia response. Its activity is regulated by the oxygen-dependent degradation of the HIF-1α protein (HIF-1A gene). Therefore, the effects of low oxygen tension in high altitude were explored using fluorescent sensors of hypoxia. METHODS: The development of the sensor provided more sensitivity for detecting hypoxia by generating a calibration of optimized parameters such as reagent concentrations, reagent volumes, and device dimensions. RESULTS: There is a high sensitivity and specificity in detecting the changes of HIF-1α protein hypoxia using the feasibility hypoxia test. This would enable point-of-care (POC) testing and individual self-administration, resulting in faster and more accurate results that can be used for a robust diagnostic approach and enhanced health surveillance, particularly in high-altitude exposure. Aerospace Medical Association 2023 Article PeerReviewed Shaharuddin, Shazreen and Nik Abd Rahman, Nik Mohd Afizan and Masarudin, Mas Jaffri and Alamassi, Mohammedarfat N. and Ahmad Saad, Fathinul Fikri (2023) HIF-1 sensor in detecting hypoxia tolerance at high altitude. Aerospace Medicine and Human Performance, 94 (6). pp. 1-6. ISSN 2375-6314; ESSN: 2375-6322 https://www.ingentaconnect.com/content/asma/amhp/2023/00000094/00000006/art00014;jsessionid=71ydx8oeue8j.x-ic-live-03 10.3357/amhp.6166.2023 |
| spellingShingle | Shaharuddin, Shazreen Nik Abd Rahman, Nik Mohd Afizan Masarudin, Mas Jaffri Alamassi, Mohammedarfat N. Ahmad Saad, Fathinul Fikri HIF-1 sensor in detecting hypoxia tolerance at high altitude |
| title | HIF-1 sensor in detecting hypoxia tolerance at high altitude |
| title_full | HIF-1 sensor in detecting hypoxia tolerance at high altitude |
| title_fullStr | HIF-1 sensor in detecting hypoxia tolerance at high altitude |
| title_full_unstemmed | HIF-1 sensor in detecting hypoxia tolerance at high altitude |
| title_short | HIF-1 sensor in detecting hypoxia tolerance at high altitude |
| title_sort | hif 1 sensor in detecting hypoxia tolerance at high altitude |
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