Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-1H NMR metabolomics study
Tilapia is one of the most common fish species that is intensively produced all over the world. However, significant measures at improving aquaculture health must be taken since disease outbreaks are often encountered in the rapidly developing aquaculture industry. Therefore, the objective of the st...
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| Format: | Article |
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Elsevier
2023
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| _version_ | 1811137853533454336 |
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| author | Ahamad Bustamam, Muhammad Safwan Pantami, Hamza Ahmed Shaari, Khozirah Chong, Chou Min Mediani, Ahmed Ismail, Intan Safinar |
| author_facet | Ahamad Bustamam, Muhammad Safwan Pantami, Hamza Ahmed Shaari, Khozirah Chong, Chou Min Mediani, Ahmed Ismail, Intan Safinar |
| author_sort | Ahamad Bustamam, Muhammad Safwan |
| collection | UPM |
| description | Tilapia is one of the most common fish species that is intensively produced all over the world. However, significant measures at improving aquaculture health must be taken since disease outbreaks are often encountered in the rapidly developing aquaculture industry. Therefore, the objective of the study was designed to evaluate the metabolite changes in tilapia’ sera through 1H NMR metabolomics in identifying the potential biomarkers responsible for immunomodulatory effect by the indigenous species of Malaysian microalgae Isochrysis galbana (IG). The results showed that IG-incorporated diet mainly at 5.0% has improved the immune response of innate immunity as observed in serum bactericidal activity (SBA) and serum lysozyme activity (SLA). The orthogonal partial least squares (OPLS) analysis indicated 5 important metabolites significantly upregulated namely as ethanol, lipoprotein, lipid, α-glucose and unsaturated fatty acid (UFA) in the 5.0% IG-incorporated diet compared to control. In conclusion, this study had successfully determined IG in improving aquaculture health through its potential use as an immune modulator. This work also demonstrated the effective use of metabolomics approach in the development of alternative nutritious diet from microalgae species to boost fish health in fulfilling the aquaculture's long-term goals. |
| first_indexed | 2024-09-25T03:40:54Z |
| format | Article |
| id | upm.eprints-108275 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:40:54Z |
| publishDate | 2023 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-1082752024-09-10T07:53:32Z http://psasir.upm.edu.my/id/eprint/108275/ Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-1H NMR metabolomics study Ahamad Bustamam, Muhammad Safwan Pantami, Hamza Ahmed Shaari, Khozirah Chong, Chou Min Mediani, Ahmed Ismail, Intan Safinar Tilapia is one of the most common fish species that is intensively produced all over the world. However, significant measures at improving aquaculture health must be taken since disease outbreaks are often encountered in the rapidly developing aquaculture industry. Therefore, the objective of the study was designed to evaluate the metabolite changes in tilapia’ sera through 1H NMR metabolomics in identifying the potential biomarkers responsible for immunomodulatory effect by the indigenous species of Malaysian microalgae Isochrysis galbana (IG). The results showed that IG-incorporated diet mainly at 5.0% has improved the immune response of innate immunity as observed in serum bactericidal activity (SBA) and serum lysozyme activity (SLA). The orthogonal partial least squares (OPLS) analysis indicated 5 important metabolites significantly upregulated namely as ethanol, lipoprotein, lipid, α-glucose and unsaturated fatty acid (UFA) in the 5.0% IG-incorporated diet compared to control. In conclusion, this study had successfully determined IG in improving aquaculture health through its potential use as an immune modulator. This work also demonstrated the effective use of metabolomics approach in the development of alternative nutritious diet from microalgae species to boost fish health in fulfilling the aquaculture's long-term goals. Elsevier 2023 Article PeerReviewed Ahamad Bustamam, Muhammad Safwan and Pantami, Hamza Ahmed and Shaari, Khozirah and Chong, Chou Min and Mediani, Ahmed and Ismail, Intan Safinar (2023) Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-1H NMR metabolomics study. Fish and Shellfish Immunology, 132. art. no. 108455. pp. 1-11. ISSN 1050-4648; ESSN: 1095-9947 https://linkinghub.elsevier.com/retrieve/pii/S1050464822007884 10.1016/j.fsi.2022.108455 |
| spellingShingle | Ahamad Bustamam, Muhammad Safwan Pantami, Hamza Ahmed Shaari, Khozirah Chong, Chou Min Mediani, Ahmed Ismail, Intan Safinar Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-1H NMR metabolomics study |
| title | Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-1H NMR metabolomics study |
| title_full | Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-1H NMR metabolomics study |
| title_fullStr | Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-1H NMR metabolomics study |
| title_full_unstemmed | Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-1H NMR metabolomics study |
| title_short | Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-1H NMR metabolomics study |
| title_sort | immunomodulatory effects of isochrysis galbana incorporated diet on oreochromis sp red hybrid tilapia via sera 1h nmr metabolomics study |
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