The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era
Streptococcus pneumoniae (pneumococcus) belongs to the Gram-positive coccus category. This bacterium typically colonises the nasopharyngeal region of healthy individuals. It comprises an extremely distinct polysaccharide capsule adding to virulence, allowing the bacteria to elude the immune defence...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Polish Society of Microbiologists
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/108735/1/108735.pdf |
| _version_ | 1817927578563903488 |
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| author | Abdul Rahman, Nurul Asyikin Mohd Desa, Mohd Nasir Masri, Siti Norbaya Taib, Niazlin Mohd Sulaiman, Nurshahira Hazman, Hazmin John, James |
| author_facet | Abdul Rahman, Nurul Asyikin Mohd Desa, Mohd Nasir Masri, Siti Norbaya Taib, Niazlin Mohd Sulaiman, Nurshahira Hazman, Hazmin John, James |
| author_sort | Abdul Rahman, Nurul Asyikin |
| collection | UPM |
| description | Streptococcus pneumoniae (pneumococcus) belongs to the Gram-positive coccus category. This bacterium typically colonises the nasopharyngeal region of healthy individuals. It comprises an extremely distinct polysaccharide capsule adding to virulence, allowing the bacteria to elude the immune defence mechanisms. Consequently, it might trigger aggressive conditions like septicaemia and meningitis in immunocompromised or older individuals; moreover, children below five years of age, are at risk of morbidity and mortality. Studies have found 101 S. pneumoniae capsular serotypes, of which several correlate with clinical and carriage isolates with distinct disease aggressiveness. The introduction of pneumococcal conjugate vaccines (PCV) targets the most common disease-associated serotypes. Nevertheless, vaccine selection pressure leads to replacement of the formerly dominant vaccine serotypes (VTs) by non-vaccine types (NVTs). Therefore, serotyping must be conducted for epidemiological surveillance and vaccine assessment. Serotyping can be performed using numerous techniques either by the conventional antisera-based (Quellung and latex agglutination) or molecular based approaches (sequetyping, multiplex PCR, realtime PCR and PCR-RFLP). A cost-effective and practical approach must be used to enhance serotyping accuracy to monitor the prevalence of VTs and NVTs. Therefore, dependable pneumococcal serotyping techniques are essential to precisely monitor virulent lineages, NVT emergence, and genetic associations of isolates. This review discusses the principles, the associated benefits and drawbacks for the respective available conventional and molecular approaches, and potentially the whole genome sequencing (WGS) to be directed for future exploration. |
| first_indexed | 2024-12-09T02:20:40Z |
| format | Article |
| id | upm.eprints-108735 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-12-09T02:20:40Z |
| publishDate | 2023 |
| publisher | Polish Society of Microbiologists |
| record_format | dspace |
| spelling | upm.eprints-1087352024-10-11T08:57:17Z http://psasir.upm.edu.my/id/eprint/108735/ The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era Abdul Rahman, Nurul Asyikin Mohd Desa, Mohd Nasir Masri, Siti Norbaya Taib, Niazlin Mohd Sulaiman, Nurshahira Hazman, Hazmin John, James Streptococcus pneumoniae (pneumococcus) belongs to the Gram-positive coccus category. This bacterium typically colonises the nasopharyngeal region of healthy individuals. It comprises an extremely distinct polysaccharide capsule adding to virulence, allowing the bacteria to elude the immune defence mechanisms. Consequently, it might trigger aggressive conditions like septicaemia and meningitis in immunocompromised or older individuals; moreover, children below five years of age, are at risk of morbidity and mortality. Studies have found 101 S. pneumoniae capsular serotypes, of which several correlate with clinical and carriage isolates with distinct disease aggressiveness. The introduction of pneumococcal conjugate vaccines (PCV) targets the most common disease-associated serotypes. Nevertheless, vaccine selection pressure leads to replacement of the formerly dominant vaccine serotypes (VTs) by non-vaccine types (NVTs). Therefore, serotyping must be conducted for epidemiological surveillance and vaccine assessment. Serotyping can be performed using numerous techniques either by the conventional antisera-based (Quellung and latex agglutination) or molecular based approaches (sequetyping, multiplex PCR, realtime PCR and PCR-RFLP). A cost-effective and practical approach must be used to enhance serotyping accuracy to monitor the prevalence of VTs and NVTs. Therefore, dependable pneumococcal serotyping techniques are essential to precisely monitor virulent lineages, NVT emergence, and genetic associations of isolates. This review discusses the principles, the associated benefits and drawbacks for the respective available conventional and molecular approaches, and potentially the whole genome sequencing (WGS) to be directed for future exploration. Polish Society of Microbiologists 2023-06-01 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/108735/1/108735.pdf Abdul Rahman, Nurul Asyikin and Mohd Desa, Mohd Nasir and Masri, Siti Norbaya and Taib, Niazlin Mohd and Sulaiman, Nurshahira and Hazman, Hazmin and John, James (2023) The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era. Polish Journal of Microbiology, 72 (2). pp. 103-115. ISSN 1733-1331; eISSN: 2544-4646 https://sciendo.com/article/10.33073/pjm-2023-023 10.33073/pjm-2023-023 |
| spellingShingle | Abdul Rahman, Nurul Asyikin Mohd Desa, Mohd Nasir Masri, Siti Norbaya Taib, Niazlin Mohd Sulaiman, Nurshahira Hazman, Hazmin John, James The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era |
| title | The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era |
| title_full | The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era |
| title_fullStr | The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era |
| title_full_unstemmed | The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era |
| title_short | The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era |
| title_sort | molecular approaches and challenges of streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era |
| url | http://psasir.upm.edu.my/id/eprint/108735/1/108735.pdf |
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