Promoter cis-element analyses reveal the function of αvpe in drought stress response of arabidopsis
<jats:p>Vacuolar processing enzyme (VPE) is a cysteine protease responsible for vacuolar proteins’ maturation and regulation of programmed cell death (PCD). Four isoforms of Arabidopsis thaliana VPEs were identified previously, but only the functions of βVPE, γVPE, and δVPE were determined. Th...
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| Format: | Article |
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MDPI
2023
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| _version_ | 1811137876372488192 |
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| author | Tang, Chu-Nie Wan Abdullah, Wan Muhamad Asrul Nizam Wee, Chien-Yeong Balia Yusof, Zetty Norhana Yap, Wai-Sum Cheng, Wan-Hee Baharum, Nadiya Akmal Ong-Abdullah, Janna Loh, Jiun-Yan Lai, Kok-Song |
| author_facet | Tang, Chu-Nie Wan Abdullah, Wan Muhamad Asrul Nizam Wee, Chien-Yeong Balia Yusof, Zetty Norhana Yap, Wai-Sum Cheng, Wan-Hee Baharum, Nadiya Akmal Ong-Abdullah, Janna Loh, Jiun-Yan Lai, Kok-Song |
| author_sort | Tang, Chu-Nie |
| collection | UPM |
| description | <jats:p>Vacuolar processing enzyme (VPE) is a cysteine protease responsible for vacuolar proteins’ maturation and regulation of programmed cell death (PCD). Four isoforms of Arabidopsis thaliana VPEs were identified previously, but only the functions of βVPE, γVPE, and δVPE were determined. The specific function of a gene is linked to the cis-acting elements in the promoter region. A promoter analysis found repetitive drought-related cis-elements in αVPE, which highlight its potential involvement in drought regulation in A. thaliana. The further co-expression network portraying genes interacting with αVPE substantiated its drought-regulation-related function. Expression of αVPE was upregulated after drought treatment in A. thaliana. To confirm the role of αVPE, a loss of function study revealed that αVPE knockout mutants remained green compared with WT after drought treatment. The mutants had reduced proline activity, decreased sucrose content, and lower MDA content, but increased photosynthetic pigments, indicating that αVPE negatively regulates drought tolerance in A. thaliana. Taken together, our findings serve as important evidence of the involvement of αVPE in modulating drought tolerance in A. thaliana.</jats:p> |
| first_indexed | 2024-09-25T03:41:16Z |
| format | Article |
| id | upm.eprints-108971 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:41:16Z |
| publishDate | 2023 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-1089712024-05-17T02:36:40Z http://psasir.upm.edu.my/id/eprint/108971/ Promoter cis-element analyses reveal the function of αvpe in drought stress response of arabidopsis Tang, Chu-Nie Wan Abdullah, Wan Muhamad Asrul Nizam Wee, Chien-Yeong Balia Yusof, Zetty Norhana Yap, Wai-Sum Cheng, Wan-Hee Baharum, Nadiya Akmal Ong-Abdullah, Janna Loh, Jiun-Yan Lai, Kok-Song <jats:p>Vacuolar processing enzyme (VPE) is a cysteine protease responsible for vacuolar proteins’ maturation and regulation of programmed cell death (PCD). Four isoforms of Arabidopsis thaliana VPEs were identified previously, but only the functions of βVPE, γVPE, and δVPE were determined. The specific function of a gene is linked to the cis-acting elements in the promoter region. A promoter analysis found repetitive drought-related cis-elements in αVPE, which highlight its potential involvement in drought regulation in A. thaliana. The further co-expression network portraying genes interacting with αVPE substantiated its drought-regulation-related function. Expression of αVPE was upregulated after drought treatment in A. thaliana. To confirm the role of αVPE, a loss of function study revealed that αVPE knockout mutants remained green compared with WT after drought treatment. The mutants had reduced proline activity, decreased sucrose content, and lower MDA content, but increased photosynthetic pigments, indicating that αVPE negatively regulates drought tolerance in A. thaliana. Taken together, our findings serve as important evidence of the involvement of αVPE in modulating drought tolerance in A. thaliana.</jats:p> MDPI 2023-03-10 Article PeerReviewed Tang, Chu-Nie and Wan Abdullah, Wan Muhamad Asrul Nizam and Wee, Chien-Yeong and Balia Yusof, Zetty Norhana and Yap, Wai-Sum and Cheng, Wan-Hee and Baharum, Nadiya Akmal and Ong-Abdullah, Janna and Loh, Jiun-Yan and Lai, Kok-Song (2023) Promoter cis-element analyses reveal the function of αvpe in drought stress response of arabidopsis. Biology, 12 (3). art. no. 430. pp. 1-10. ISSN 2079-7737 https://www.mdpi.com/2079-7737/12/3/430 10.3390/biology12030430 |
| spellingShingle | Tang, Chu-Nie Wan Abdullah, Wan Muhamad Asrul Nizam Wee, Chien-Yeong Balia Yusof, Zetty Norhana Yap, Wai-Sum Cheng, Wan-Hee Baharum, Nadiya Akmal Ong-Abdullah, Janna Loh, Jiun-Yan Lai, Kok-Song Promoter cis-element analyses reveal the function of αvpe in drought stress response of arabidopsis |
| title | Promoter cis-element analyses reveal the function of αvpe in drought stress response of arabidopsis |
| title_full | Promoter cis-element analyses reveal the function of αvpe in drought stress response of arabidopsis |
| title_fullStr | Promoter cis-element analyses reveal the function of αvpe in drought stress response of arabidopsis |
| title_full_unstemmed | Promoter cis-element analyses reveal the function of αvpe in drought stress response of arabidopsis |
| title_short | Promoter cis-element analyses reveal the function of αvpe in drought stress response of arabidopsis |
| title_sort | promoter cis element analyses reveal the function of αvpe in drought stress response of arabidopsis |
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