LC-MS/MS and GC-MS analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of Lygodium microphyllum (Cav.) R. Br
Natural products serve as a valuable source of antioxidants with potential health benefits for various conditions. Lygodium microphyllum (Cav.) R. Br., also known as Old World climbing fern, is an invasive climbing fern native to Southeast Asia, Africa, South America, Australia, and Melanesia. It ha...
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| Format: | Article |
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MDPI
2023
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| _version_ | 1825939696698523648 |
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| author | Teoh, Wuen Yew Yong, Yoong Soon Razali, Faizan Naeem Stephenie, Sarah Shah, Muhammad Dawood Tan, Jen Kit Gnanaraj, Charles Mohd Esa, Norhaizan |
| author_facet | Teoh, Wuen Yew Yong, Yoong Soon Razali, Faizan Naeem Stephenie, Sarah Shah, Muhammad Dawood Tan, Jen Kit Gnanaraj, Charles Mohd Esa, Norhaizan |
| author_sort | Teoh, Wuen Yew |
| collection | UPM |
| description | Natural products serve as a valuable source of antioxidants with potential health benefits for various conditions. Lygodium microphyllum (Cav.) R. Br., also known as Old World climbing fern, is an invasive climbing fern native to Southeast Asia, Africa, South America, Australia, and Melanesia. It has been reported to possess interesting pharmacological properties including hepatoprotective and anti-inflammatory mechanisms. This study analyzed the potential bioactive metabolites that contribute to the antioxidant and antimicrobial effects of L. microphyllum (LM) by profiling the crude extract using high-resolution LC-MS/MS and GC-MS systems. Several classes of compounds such as phenolics, flavonoids, terpenoids, steroids, macrolides, vitamins, lipids, and other hydrocarbons were found in the crude extract of LM through non-targeted analysis. A total of 74 compounds were detected in LC-MS/MS, whereas a total of nine compounds were identified in GC-MS. Out of the 74 compounds detected in LC-MS/MS, 34 compounds, primarily quercetin, kaempferol, trifolin, pyroglutamic acid, arachidonic acid, and rutin were reported with antioxidant, antimicrobial, anti-inflammatory, and hepatoprotective activities. The presence of phenolic and flavonoid compounds with reported bioactivities in the crude extract of LM evidence its pharmacological properties. |
| first_indexed | 2024-12-09T02:21:09Z |
| format | Article |
| id | upm.eprints-109015 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-12-09T02:21:09Z |
| publishDate | 2023 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-1090152024-10-15T05:42:49Z http://psasir.upm.edu.my/id/eprint/109015/ LC-MS/MS and GC-MS analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of Lygodium microphyllum (Cav.) R. Br Teoh, Wuen Yew Yong, Yoong Soon Razali, Faizan Naeem Stephenie, Sarah Shah, Muhammad Dawood Tan, Jen Kit Gnanaraj, Charles Mohd Esa, Norhaizan Natural products serve as a valuable source of antioxidants with potential health benefits for various conditions. Lygodium microphyllum (Cav.) R. Br., also known as Old World climbing fern, is an invasive climbing fern native to Southeast Asia, Africa, South America, Australia, and Melanesia. It has been reported to possess interesting pharmacological properties including hepatoprotective and anti-inflammatory mechanisms. This study analyzed the potential bioactive metabolites that contribute to the antioxidant and antimicrobial effects of L. microphyllum (LM) by profiling the crude extract using high-resolution LC-MS/MS and GC-MS systems. Several classes of compounds such as phenolics, flavonoids, terpenoids, steroids, macrolides, vitamins, lipids, and other hydrocarbons were found in the crude extract of LM through non-targeted analysis. A total of 74 compounds were detected in LC-MS/MS, whereas a total of nine compounds were identified in GC-MS. Out of the 74 compounds detected in LC-MS/MS, 34 compounds, primarily quercetin, kaempferol, trifolin, pyroglutamic acid, arachidonic acid, and rutin were reported with antioxidant, antimicrobial, anti-inflammatory, and hepatoprotective activities. The presence of phenolic and flavonoid compounds with reported bioactivities in the crude extract of LM evidence its pharmacological properties. MDPI 2023-03-20 Article PeerReviewed Teoh, Wuen Yew and Yong, Yoong Soon and Razali, Faizan Naeem and Stephenie, Sarah and Shah, Muhammad Dawood and Tan, Jen Kit and Gnanaraj, Charles and Mohd Esa, Norhaizan (2023) LC-MS/MS and GC-MS analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of Lygodium microphyllum (Cav.) R. Br. Separations, 10 (3). art. no. 215. pp. 1-12. ISSN 2297-8739 https://www.mdpi.com/2297-8739/10/3/215 10.3390/separations10030215 |
| spellingShingle | Teoh, Wuen Yew Yong, Yoong Soon Razali, Faizan Naeem Stephenie, Sarah Shah, Muhammad Dawood Tan, Jen Kit Gnanaraj, Charles Mohd Esa, Norhaizan LC-MS/MS and GC-MS analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of Lygodium microphyllum (Cav.) R. Br |
| title | LC-MS/MS and GC-MS analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of Lygodium microphyllum (Cav.) R. Br |
| title_full | LC-MS/MS and GC-MS analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of Lygodium microphyllum (Cav.) R. Br |
| title_fullStr | LC-MS/MS and GC-MS analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of Lygodium microphyllum (Cav.) R. Br |
| title_full_unstemmed | LC-MS/MS and GC-MS analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of Lygodium microphyllum (Cav.) R. Br |
| title_short | LC-MS/MS and GC-MS analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of Lygodium microphyllum (Cav.) R. Br |
| title_sort | lc ms ms and gc ms analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of lygodium microphyllum cav r br |
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