Tapered optical fiber DNA biosensor for detecting Leptospira DNA
Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene. Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 1...
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| Format: | Article |
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Medknow Publications
2023
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| _version_ | 1825939708199305216 |
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| author | Lam, Jia-Yong Yaacob, Mohd Hanif Chee, Hui-Yee |
| author_facet | Lam, Jia-Yong Yaacob, Mohd Hanif Chee, Hui-Yee |
| author_sort | Lam, Jia-Yong |
| collection | UPM |
| description | Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene. Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 μm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum. Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/μL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach. Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis. |
| first_indexed | 2024-09-25T03:41:18Z |
| format | Article |
| id | upm.eprints-109080 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:41:18Z |
| publishDate | 2023 |
| publisher | Medknow Publications |
| record_format | dspace |
| spelling | upm.eprints-1090802024-09-02T06:51:25Z http://psasir.upm.edu.my/id/eprint/109080/ Tapered optical fiber DNA biosensor for detecting Leptospira DNA Lam, Jia-Yong Yaacob, Mohd Hanif Chee, Hui-Yee Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene. Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 μm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum. Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/μL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach. Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis. Medknow Publications 2023-03 Article PeerReviewed Lam, Jia-Yong and Yaacob, Mohd Hanif and Chee, Hui-Yee (2023) Tapered optical fiber DNA biosensor for detecting Leptospira DNA. Asian Pacific Journal of Tropical Medicine, 16 (3). pp. 119-128. ISSN 1995-7645; ESSN: 2352-4146 https://journals.lww.com/aptm/fulltext/2023/16030/tapered_optical_fiber_dna_biosensor_for_detecting.5.aspx 10.4103/1995-7645.372293 |
| spellingShingle | Lam, Jia-Yong Yaacob, Mohd Hanif Chee, Hui-Yee Tapered optical fiber DNA biosensor for detecting Leptospira DNA |
| title | Tapered optical fiber DNA biosensor for detecting Leptospira DNA |
| title_full | Tapered optical fiber DNA biosensor for detecting Leptospira DNA |
| title_fullStr | Tapered optical fiber DNA biosensor for detecting Leptospira DNA |
| title_full_unstemmed | Tapered optical fiber DNA biosensor for detecting Leptospira DNA |
| title_short | Tapered optical fiber DNA biosensor for detecting Leptospira DNA |
| title_sort | tapered optical fiber dna biosensor for detecting leptospira dna |
| work_keys_str_mv | AT lamjiayong taperedopticalfiberdnabiosensorfordetectingleptospiradna AT yaacobmohdhanif taperedopticalfiberdnabiosensorfordetectingleptospiradna AT cheehuiyee taperedopticalfiberdnabiosensorfordetectingleptospiradna |