Production of biosurfactant using Bacillus subtilis Natto fermentation
Biosurfactants are microbial amphiphiles produced as primary metabolites by varieties of microorganisms. They are preferred over chemically derived surfactants owing to their intrinsic properties, such as superior environmental compatibility, biodegradability, anti-inflammatory and antimicrobial act...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/109104/1/05%20JST-3655-2022.pdf |
| _version_ | 1811137879400775680 |
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| author | Leow, Yew Seng Abdullah, Norhafizah Awang Biak, Dayang Radiah Jamali, Nur Syakina Rosli, Rozita Teh, Huey Fang |
| author_facet | Leow, Yew Seng Abdullah, Norhafizah Awang Biak, Dayang Radiah Jamali, Nur Syakina Rosli, Rozita Teh, Huey Fang |
| author_sort | Leow, Yew Seng |
| collection | UPM |
| description | Biosurfactants are microbial amphiphiles produced as primary metabolites by varieties of microorganisms. They are preferred over chemically derived surfactants owing to their intrinsic properties, such as superior environmental compatibility, biodegradability, anti-inflammatory and antimicrobial activity, and higher tolerance towards extreme environmental conditions such as temperature, salinity, and pH levels. However, commercial production of biosurfactants is still lacking. The main reason for this is the low yields obtained from fermentation processes, which causes them to be unable to compete compared to chemical surfactants. The present study conducted a one-factor-at-a-time (OFAT) analysis on fermentation conditions to enhance biosurfactant yield from a probiotic strain, Bacillus subtilis Natto. The fermentation was conducted by varying parameters such as nitrogen source, vegetable oils, inoculum size, amino acids, and pH of the fermentation medium. Results showed a significant improvement of 45% in biosurfactant production from B. subtilis Natto when the initial pH of the fermentation medium was adjusted to pH 6.8, urea as the nitrogen source, inoculum size of 6% v/v and the addition of palm olein at a concentration of 2% v/v as a substrate in the fermentation medium. |
| first_indexed | 2024-09-25T03:41:19Z |
| format | Article |
| id | upm.eprints-109104 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-09-25T03:41:19Z |
| publishDate | 2023 |
| publisher | Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-1091042024-09-02T06:33:32Z http://psasir.upm.edu.my/id/eprint/109104/ Production of biosurfactant using Bacillus subtilis Natto fermentation Leow, Yew Seng Abdullah, Norhafizah Awang Biak, Dayang Radiah Jamali, Nur Syakina Rosli, Rozita Teh, Huey Fang Biosurfactants are microbial amphiphiles produced as primary metabolites by varieties of microorganisms. They are preferred over chemically derived surfactants owing to their intrinsic properties, such as superior environmental compatibility, biodegradability, anti-inflammatory and antimicrobial activity, and higher tolerance towards extreme environmental conditions such as temperature, salinity, and pH levels. However, commercial production of biosurfactants is still lacking. The main reason for this is the low yields obtained from fermentation processes, which causes them to be unable to compete compared to chemical surfactants. The present study conducted a one-factor-at-a-time (OFAT) analysis on fermentation conditions to enhance biosurfactant yield from a probiotic strain, Bacillus subtilis Natto. The fermentation was conducted by varying parameters such as nitrogen source, vegetable oils, inoculum size, amino acids, and pH of the fermentation medium. Results showed a significant improvement of 45% in biosurfactant production from B. subtilis Natto when the initial pH of the fermentation medium was adjusted to pH 6.8, urea as the nitrogen source, inoculum size of 6% v/v and the addition of palm olein at a concentration of 2% v/v as a substrate in the fermentation medium. Universiti Putra Malaysia 2023-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/109104/1/05%20JST-3655-2022.pdf Leow, Yew Seng and Abdullah, Norhafizah and Awang Biak, Dayang Radiah and Jamali, Nur Syakina and Rosli, Rozita and Teh, Huey Fang (2023) Production of biosurfactant using Bacillus subtilis Natto fermentation. Pertanika Journal of Science and Technology, 31 (2). pp. 709-728. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-3655-2022 10.47836/pjst.31.2.05 |
| spellingShingle | Leow, Yew Seng Abdullah, Norhafizah Awang Biak, Dayang Radiah Jamali, Nur Syakina Rosli, Rozita Teh, Huey Fang Production of biosurfactant using Bacillus subtilis Natto fermentation |
| title | Production of biosurfactant using Bacillus subtilis Natto fermentation |
| title_full | Production of biosurfactant using Bacillus subtilis Natto fermentation |
| title_fullStr | Production of biosurfactant using Bacillus subtilis Natto fermentation |
| title_full_unstemmed | Production of biosurfactant using Bacillus subtilis Natto fermentation |
| title_short | Production of biosurfactant using Bacillus subtilis Natto fermentation |
| title_sort | production of biosurfactant using bacillus subtilis natto fermentation |
| url | http://psasir.upm.edu.my/id/eprint/109104/1/05%20JST-3655-2022.pdf |
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