Large animal models for cardiac remuscularization studies: a methodological review
Myocardial infarction is the most common cause of heart failure, one of the most fatal non-communicable diseases worldwide. The disease could potentially be treated if the dead, ischemic heart tissues are regenerated and replaced with viable and functional cardiomyocytes. Pluripotent stem cells have...
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| Format: | Article |
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Frontiers Media
2023
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| _version_ | 1811137937552703488 |
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| author | Yu, Yuexin Tham, Seng Kong Roslan, Fatin Fazrina Shaharuddin, Bakiah Yong, Yoke Keong Guo, Zhikun Tan, Jun Jie |
| author_facet | Yu, Yuexin Tham, Seng Kong Roslan, Fatin Fazrina Shaharuddin, Bakiah Yong, Yoke Keong Guo, Zhikun Tan, Jun Jie |
| author_sort | Yu, Yuexin |
| collection | UPM |
| description | Myocardial infarction is the most common cause of heart failure, one of the most fatal non-communicable diseases worldwide. The disease could potentially be treated if the dead, ischemic heart tissues are regenerated and replaced with viable and functional cardiomyocytes. Pluripotent stem cells have proven the ability to derive specific and functional cardiomyocytes in large quantities for therapy. To test the remuscularization hypothesis, the strategy to model the disease in animals must resemble the pathophysiological conditions of myocardial infarction as in humans, to enable thorough testing of the safety and efficacy of the cardiomyocyte therapy before embarking on human trials. Rigorous experiments and <jats:italic>in vivo</jats:italic> findings using large mammals are increasingly important to simulate clinical reality and increase translatability into clinical practice. Hence, this review focus on large animal models which have been used in cardiac remuscularization studies using cardiomyocytes derived from human pluripotent stem cells. The commonly used methodologies in developing the myocardial infarction model, the choice of animal species, the pre-operative antiarrhythmics prophylaxis, the choice of perioperative sedative, anaesthesia and analgesia, the immunosuppressive strategies in allowing xenotransplantation, the source of cells, number and delivery method are discussed. |
| first_indexed | 2024-09-25T03:42:14Z |
| format | Article |
| id | upm.eprints-109431 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:42:14Z |
| publishDate | 2023 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | upm.eprints-1094312024-08-05T02:30:29Z http://psasir.upm.edu.my/id/eprint/109431/ Large animal models for cardiac remuscularization studies: a methodological review Yu, Yuexin Tham, Seng Kong Roslan, Fatin Fazrina Shaharuddin, Bakiah Yong, Yoke Keong Guo, Zhikun Tan, Jun Jie Myocardial infarction is the most common cause of heart failure, one of the most fatal non-communicable diseases worldwide. The disease could potentially be treated if the dead, ischemic heart tissues are regenerated and replaced with viable and functional cardiomyocytes. Pluripotent stem cells have proven the ability to derive specific and functional cardiomyocytes in large quantities for therapy. To test the remuscularization hypothesis, the strategy to model the disease in animals must resemble the pathophysiological conditions of myocardial infarction as in humans, to enable thorough testing of the safety and efficacy of the cardiomyocyte therapy before embarking on human trials. Rigorous experiments and <jats:italic>in vivo</jats:italic> findings using large mammals are increasingly important to simulate clinical reality and increase translatability into clinical practice. Hence, this review focus on large animal models which have been used in cardiac remuscularization studies using cardiomyocytes derived from human pluripotent stem cells. The commonly used methodologies in developing the myocardial infarction model, the choice of animal species, the pre-operative antiarrhythmics prophylaxis, the choice of perioperative sedative, anaesthesia and analgesia, the immunosuppressive strategies in allowing xenotransplantation, the source of cells, number and delivery method are discussed. Frontiers Media 2023-03-15 Article PeerReviewed Yu, Yuexin and Tham, Seng Kong and Roslan, Fatin Fazrina and Shaharuddin, Bakiah and Yong, Yoke Keong and Guo, Zhikun and Tan, Jun Jie (2023) Large animal models for cardiac remuscularization studies: a methodological review. Frontiers in Cardiovascular Medicine, 10. ISSN 2297-055X https://www.frontiersin.org/articles/10.3389/fcvm.2023.1011880/full 10.3389/fcvm.2023.1011880 |
| spellingShingle | Yu, Yuexin Tham, Seng Kong Roslan, Fatin Fazrina Shaharuddin, Bakiah Yong, Yoke Keong Guo, Zhikun Tan, Jun Jie Large animal models for cardiac remuscularization studies: a methodological review |
| title | Large animal models for cardiac remuscularization studies: a methodological review |
| title_full | Large animal models for cardiac remuscularization studies: a methodological review |
| title_fullStr | Large animal models for cardiac remuscularization studies: a methodological review |
| title_full_unstemmed | Large animal models for cardiac remuscularization studies: a methodological review |
| title_short | Large animal models for cardiac remuscularization studies: a methodological review |
| title_sort | large animal models for cardiac remuscularization studies a methodological review |
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